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Psychophysiology, 1980, Vol 17, No. 5, pp. 482-493

Carotid_dP.doc


Carotid dP/dt as a Psychophysiological Index of Sympathetic Myocardial Effects: Some

Considerations

Ronald J. HESLGRAVE: and John J. FUrEdY

Department of Psychology, University of Toronto

ABSTRACT
Heart rate (HR) is modulated by both branches of the autonomic nervous system. Therefore, the neural regulation of a specific change in HR cannot be deduced from HR changes per se. For example, HR deceleration cannot be interpreted as being due to sympathetic nervous system withdrawal and/or parasympathetic nervous system activation. It is quite possible that sympathetic activation may be dominated by parasympathetic antagonism. To determine neurogenic influences on the heart, one group of researchers have focused on measuring contractility aspects of ventricular function since it has been demonstrated that the ventricles are sympathetically dominated. This paper assesses the validity of contractility-based dP/dt measures as indices of ventricular function, and thus of sympathetic activity, being especially concerned with the noninvasive carotid dP/dt measure which is of particular significance to psychophysiologists. The validation examination consists of an exploration into the underlying physiology of dP/dt measures as well as a critical appraisal of empirical psychophysiological findings related to dP/dt. Other important parameters related to psychophysiological measures, such as obtrusiveness and quan­tification, are also discussed. The conclusion is that carotid dP/dt has not been adequately validated for use by psychophysiologists and until such basic research is carried out, this psychophysiological index of sympathetic activity cannot seriously be considered a measure of sympathetic, beta-adrenergic, or even ventricular function.

DESCRIPTORS: Heart rate, dP/dt, Carotid dP/dt, Sympathetic autonomic nervous system, Parasympathetic autonomic nervous system, Contractility, Ventricular functions, Muscle mechanics, Vmax, Hemodynamics, Sympathetic myocardial measurement, Validity, Sensitivity, Obtrusiveness, Quantification, Pharmacological blockade.



Measuring myocardial function by heart rate (HR) has been common psychophysiological prac­tice if only because the HR measure is easily quan­tifiable as well as being completely unobtrusive. Physiologists and cardiologists, however, fre­quently point out that HR constitutes a "mixed" index since it is impossible to determine the relative influences of the two branches of the autonomic
The authors wish to express their appreciation to J. M. Arabian. F. Klajner, T. A. Matyas, P. A. Obrist. C. X. Poulos, D. M. Riley, and R. B. Williams and an anonymous reviewer for their valuable comments on earlier drafts of this manuscript.

Address requests for reprints to. John J. Furedy, Department of Psychology. University of Toronto, Toronto, Ontario, Canada MXS 1AI.

nervous system (ANS). The "mixed" aspect of HR becomes important whenever, as frequently hap­pens, there is an attempt to draw inferences concern­ing the respective roles of the two ANS branches based on HR alterations. For example, it may seem safe (though relatively uninformative) to interpret HR deceleration as representing parasympathetic activation and/or sympathetic withdrawal, but even such an apparently conservative interpretation may be wrong. For example, there is some evidence (Hurwitz & Furedy, 1979; Morrison & Furedy, 1980) suggesting that the initial HR deceleration that occurs reflexively during a dive preparation is produced by the combination of (dominant) para­sympathetic activation working in opposition to sympathetic activation. This instance illustrates the

point that the ability to separate the influences of the two ANS branches is critical not only for a physiological understanding of the phenomenon, but also for therapeutic applications with behavior-modification techniques such as biofeedback.

For any such separation, it is clear that any measure indexing only one neurogenic influence on the heart would be of great utility and if such a measure could be recorded noninvasively, it would be of special significance to psychophysiologists. In three recent papers in this journal (Obrist, Howard. Lawler, Sutterer, Smithson, & Martin, 1972; Ob­rist, Lawler. Howard, Smithson, Martin, & Man­ning, 1974; Obrist, Gaebelein, Teller. Langer, Grignolo, Light, & McCubbin, 1978), Obrist and his associates have offered an index of sympathetic myocardial activity in the form of the ventricular contractile force-velocity relationship of the left ventricle as measured noninvasively by the maxi­mum rate of change in increasing pressure in the carotid artery, i.e., peak dP/dt. Others, notably David Randall and his associates (Randall, Brady, & Martin, 1975), have expressed concern over the use of this index to infer neurogenic changes. They state that

theoretical accounts of the alterations in sympathetic neural in­put to the heart during classical conditioning have been provided by Obrist et al. (1972. 1974), based upon measured increases in the rate of change of arterial blood pressure or aortic blood flow. Since such measures are generally recognized to be in­fluenced as well by factors other than cardiac nerve activity (e.g., the status of the arterial vascular tree), caution must be exercised in the acceptance of such an interpretative data base, (p. 74)

Our purpose here is to assess the utility of this index by considering: a) the physiological assump­tions that underlie its use, b) the extent to which the psychophysiological evidence reported in these papers can validly be said to support the use of the index, and c) the extent to which the index satisfies the psychophysiological measurement re­quirements of unobtrusiveness and quantification.

It is important to stress that our purpose, there­fore, is highly limited. For example, Obrist and his associates have recently begun to employ pulse transit time (PTT) as another measure of myocardial contractility and hence of beta-adrenergic influ­ences on the myocardium (Obrist, Light, McCub­bin, Hutcheson, & Hoffer, 1979). We shall not directly examine PTT here, although many of the physiological problems to be raised in connection with the use of contractility indices as measures of sympathetic influences would apply to PTT as well (see also Newlin & Levenson (1979) for some discussion of problems associated with PTT).

The assessment of the physiological and psycho-

physiological-evidential considerations relevant to the carotid dP/dt measure will be in the next section labelled "validity." Then, in a briefer sec­tion we shall assess the obtrusiveness and quantifi­cation aspects of carotid dP/dt, topics of special interest to psychophysiologists. Our overall conclu­sion will be that carotid dP/dt, at least as it has been employed in the cited papers, is of such limited utility that it should be abandoned in future research as an index of ventricular contractility and beta-adrenergic influences on the myocardium.

Validity

Evidence from anatomical (Carlsten, Folkow, & Hamberger. 1957; Davies, Francis, & King, 1951), histochemical (Cooper, 1965: Hirsch, Kaiser, & Cooper, 1964, 1965; Jacobowitz, Cooper, & Barner, 1967), electrophysiological (Daggett. Nu­gent, Carr. Powers, & Harada. 1967; Hoffman & Suckling, 1953), and contractile (DeGeest. Levy, & Zieske, 1964; DeGeest, Levy. Zieske, & Lipman, 1965; Harman & Reeves, 1968: Kaye, Geesbreght, & Randall, 1970; Pace, Randall, Wechsler, & Priola, 1968; Randall & Armour, 1974a, 1974b; Randall, Pace, Wechsler, & Kim, 1969; Randall, Wechsler, Pace, & Szentivanyi. 1968; Sarnoff & Mitchell, 1962) studies indicate that parasympathet-ic influences on ventricular myocardial function are slight and sympathetic influences predominate. Al­though it is a reasonable and pragmatic approach to focus on ventricular function in the development of a sympathetic myocardial index rather than sup-raventricular (atrial) function (e.g., HR index), as one moves further and further from the effects of sympathetic activity on the ventricular myocar­dium, additional assumptions are necessary to sup­port each step distally. Fig. 1 illustrates the major inferential steps that must be taken to arrive at carotid dP/dt as an index of sympathetic activity. The validity of the connections between these steps will be considered in this section and major sources of error in the estimation of each step by its im­mediately lower step will be considered. In addi­tion, problems associated with such validation ap­proaches as pharmacological blockade will also be considered.



Parasympathetic Influences on Ventricular Contractility

As can be seen in Fig. 1, a major source of confounding in the estimation of sympathetic ner­vous system activity by ventricular myocardial con­tractility is the effects of parasympathetic nervous system stimulation on ventricular myocardial con­tractility (for an excellent review see Higgins, Vatner, & Braunwald, 1973). The early anatomical and physiological literature indicated that this source of






Fig. 1. Block diagram of physiological considerations rele­vant to the two psychophysiological indices of myocardial sym­pathetic influences. Confounding (relative to sympathetic mea­surement) sources are drawn in rectangles, while measures are depicted in triangles. See text for further details.

confounding could be ignored (and hence be deleted from Fig. 1) since this literature uniformly reported an absence of vagal innervation to the ventricular myocardium (e.g., Carlsten et al., 1957; Davies et al., 1951; Hoffman & Suckling, 1953; Sarnoff & Mitchell, 1962). However, more recent anatomical, contractile and histochemical evidence does indi­cate a parasympathetic-contractility link, although that link is less important than the sympathetic-contractility link. More specifically, while Davies et al. (1951) could not demonstrate any parasym­pathetic communication to the ventricle, electron microscopic studies by Napolitano, Willman, Hanlon, and Cooper (1965) have shown that there are parasympathetic nerve fibers to the ventricle. In addition, other indications of the presence of parasympathetic fibers in the ventricle have also been discovered. Cooper (1965) demonstrated the presence of acetylcholinesterase in ventricular tis­sue, a finding also supported by Hirsch and his associates (Hirsch et al., 1964,1965). Jacobowitz et al. (1967) offered further support for this conclusion utilizing the thiocholine method to identify acetyl­cholinesterase.

Even more relevant to the concerns of the present paper is the fact that, contrary to the claims of the earlier reports (e.g., Carlsten et al., 1957; Hoffman

& Suckling, 1953; Sarnoff & Mitchell, 1962), there are later numerous examples of parasympathetic innervation to the ventricle having functional sig­nificance when indexed by contractility. Thus, al­though Hoffman and Suckling (1953) could not demonstrate significant effects of acetylcholine on the transmembrane resting or action potentials, Eliakim, Bellet, Tawil, and Muller (1961) did dem­onstrate a negative inotropic effect with surgically produced complete heart block. Moreover, in a later study DeGeest et al. (1965) produced an even more impressive demonstration when they kept HR and other sources of confounding constant (HR being one major source of confounding for the assessment of contractility, and a source that was not controlled in the earlier studies). DeGeest et al. found that supramaximal vagal stimulation produced a 23% reduction in contractility as measured by the peak pressure of the left ventricle. Again, Daggett et al. (1967) showed the presence of vagal cholinergic innervation to the ventricular myocardium by dem­onstrating that direct vagal stimulation (which was a different stimulation technique from that of Sarnoff & Mitchell, 1962) would produce a significant re­duction in ventricular contractile strength (measured in the left ventricle by peak dP/dt). More recently, Kissling, Reutter, Sieber, Nguyen-Duong, and Jacob (cited in Levy, 1977) demonstrated a 34% reduction in contractile force by electrically induc­ing the release of endogenous acetylcholine follow­ing the administration of guanethidine.

Left and right ventricular contractility, along with peak dP/dt, have also been shown to be de­pressed by vagal stimulation in reports by Harman and Reeves (1968) and Stanton and Vick (1968). Further, a report by Kaye et al. (1970) provides a more detailed elucidation of this effect. In that study Kaye et al. (1970) found that right vagal stimulation depresses contractile force in both ventricles, while left vagal stimulation depresses only left ventricular contractile force. Priola and Fulton (1969) also demonstrated differential effects on the left and right ventricles in their finding that vagal stimulation depresses contractility from 4-12% in the right ventricle but only from 1-10% in the left ventricle. In a further extensive series of studies (Pace et al., 1968; Randall et al., 1968; Randall et al., 1969; Randall & Armour, 1974a, 1974b), W. C. Randall and his associates have demonstrated an uneven distribution of vagal effects on various regions of the ventricles; they found contractility to be more depressed at the basal portions of the ventricles than at the apex. D. C. Randall and his associates (Ran­dall, Armour, & Randall, 1971, 1972) have also shown regional differences with a mean reduction in contractile force of 25% in epicardial regions and 38% in endocardial structures.

Finally, although the above studies indicate that vagal stimulation can produce negative inotropic effects, it should be briefly noted that the effect of parasympathetic activity on ventricular contractility is more complicated and thus modifies the picture painted above. One complication is that the effect exerted by the parasympathetic system is modified by its interaction with the sympathetic system. For instance, under increased adrenergic tone, it has been found that negative inotropic antagonism is enhanced (e.g., Hollenberg, Carrierre, & Barger, 1965; Levy & Zieske, 1969a; Stanton & Vick, 1968). Another complication is that positive ino­tropic effects can occur upon cessation of vagal stimulation or during vagal stimulation in the pres­ence of atropine (e.g., Harman & Reeves, 1968; Levy & Zieske, 1969b, 1969c; Randall et al., 1968). Therefore, it can be seen that evidence is strong in support of the ability of the parasympathetic system to exert inotropic effects on the ventricle.

In terms, then, of Fig, 1, current research has shown the effect of parasympathetic (cholinergic) activity on ventricular function to be sufficiently significant to justify the inclusion of a PNS-contractility link in that figure. This is not to deny that, in initial, pragmatic terms, it is preferable to focus on ventricular rather than supraventricular functions in the development of any valid index of sympathetic activity. It is only to state that, contrary to earlier indications, parasympathetic influences on contractility cannot be ruled out. To that extent, the first 'Inferential step" from sympathetic activ­ity to carotid dP/dt has to be viewed as a potentially halting one. On the other hand, there may be a specifiable set of conditions under which parasym­pathetic influences on ventricular contractile func­tion are negligible. In that case, the validity of carotid dP/dt would require the examination of the soundness of the later inferential steps depicted in Fig. 1. Of these steps the second, dealing with the problem of directly measured contractility (i.e., the link in Fig. 1 between contractility and ventricular dP/dt), is the subject of examination in our next subsection.

Direct Ventricular Measures and the Problem of Preload and Afterload Factors

The term ' 'direct'' is used in the anatomical sense to mean that the measure is taken from ventricular loci. Ventricular contractility can be viewed either from a muscle-mechanics or a hemodynamic point of view (see Fig. 1). We will first consider the muscle mechanics point of view both because the correction for loading factors has consensus and, as will be seen later, Obrist et al. (1972) incorporate muscle-mechanical definitions of contractility in their basic validation study. From this point of view

the ventricle is considered not as a pump but as a muscle, and the logic of basic muscle mechanics is used to assess contractility. The simplest muscle-mechanical index is the velocity of muscle contrac­tion (Vmc) where the muscle in question is the set of fibers in the ventricular myocardium. Unfortu­nately, as indicated in Fig. 1, Vmc is affected not only by contractility but also by preload and after-load factors.

The effects of loading factors may be seen by applying Hill's (1938) three component conceptual analysis of the mechanical properties of skeletal muscle. In this model muscle contraction behaves as if there were: a contractile element (CE) which at rest is freely extensible but activation causes it to develop force and shorten; a series elastic compo­nent (SE) which is passively stretched by the short­ening of the CE; and a parallel elastic component (PE) which is arranged in parallel with CE and supports resting tension.

Recent work has indicated that Hill's three com­ponent model accounts for a substantial body of experimental evidence (Parmley & Sonnenblick, 1967; Parmley, Spann, Taylor, & Sonnenblick, 1968). In terms of the model, the aim is to estimate contractility (viewed as the shortening of the CE) independently of other factors such as preload, corresponding to the diastolic stretch of the myofilaments or end-diastolic pressure, and afterload, corresponding to aortic pressure. To understand why Vmax (see Fig. 1) is claimed to be an unconfounded measure of contractility, consider first the inverse relationship between force and velocity which is fundamental to muscle mechanics: as after­load (force) increases, the initial velocity of shorten­ing of CE, as well as the extent of shortening, decreases. The force-velocity curve that results from the relationship between afterload and velocity takes into account the afterload factor, but this curve still reflects two fundamental ways in which the myocardium can be altered: a) by changing contrac­tile state, and b) by changing the initial muscle fiber length (preload). Given that one wishes to estimate only the former factor (changes in the contractile state), and that the force-velocity curve shifts with variations in the latter preload factor (Sonnenblick, Parmley, & Urschel, 1969), the velocity of shorten­ing of the ventricular myocardium with a known afterload is still insufficient to estimate CE. How­ever, it has been demonstrated that although a family of force-velocity curves result from variation in preload (at a given afterload), these curves all asymptote on the axis of velocity at the same point. Therefore, this point of maximal velocity of short­ening of CE (Vmax), which is determined by extrapolating the force-velocity curves to zero load, is independent of preload (Sonnenblick, 1962a,

1962b; Sonnenblick et al., 1969; Mason, Zelis, Amsterdam, & Massumi, 1974). Thus, the value of Vmax (mm/sec) varies directly and uniquely with the contractile state of the myocardium.

It bears emphasis that Vmax is not without some problems (Noble, 1972); principally it has been questioned whether the model can be applied to an intact heart since little is known about some of the factors necessary to calculate Vmax, such as the force-stretch relationship of SE. Still others (Hux­ley & Simmons, 1971) question the applicability of this model to the myocardium and even to skeletal muscle. Nevertheless, because of its independence from preload and afterload factors, this measure is generally considered to be an unconfounded and sensitive indication of change in the contractile state of the myocardium. On the other hand, because Vmax is a direct measure of ventricular contractil­ity, it has not been employed by psychophysiologists as a sympathetic index simply because of its invasive characteristic. However, Vmax may still be very useful as a method for validating other less direct and more psychophysiologically appropriate measures of contractility and hence of sympathetic influences. The simpler Vmc index, on the other hand, does not enjoy even such potential usefulness because there has been no correction for preload and afterload factors (see Fig. 1) in that estimate of contractility.

The other approach to ventricular contractility is to focus on its hemodynamic aspects (see Fig. 1), wherein the ventricle is considered as a pump and contractility is directly assessed as the maximum rate of increase in intraventricular pressure ex­pressed as peak dP/dt which usually corresponds to the opening of the semilunar valves. Like the muscle-mechanical Vmc measure, and as suggested in Fig. 1, this hemodynamic intraventricular dP/dt index is affected and confounded by preload and afterload factors. In the hemodynamically oriented literature there have, in fact, been several corrective recommendations that have been put forward and examined. One set of recommended transforma­tions is designed to correct for preload alone and comprises ratio formulae with peak dP/dt as one term and integrated systolic isometric tension (Siegel & Sonnenblick, 1963), integrated isovolumetric pressure (Veragut & Krayenbuhl, 1965), maximal isovolumetric tension (Frank & Levin-son, 1964), or end-diastolic pressure (Reeves, Hefner, Jones, Coghlan, Prieto, & Carroll, 1960) as the other term. There appears to be more consensus concerning the problem of correcting for afterload alone, it being widely agreed that dividing peak dP/dt by developed isovolumetric pressure—a commonly used measure—satisfactorily corrects for afterload variations (Mason, 1969; Mason,

Braunwald, Covell, Sonnenblick, & Ross, 1971; Mason et al., 1974). Finally, there has also been a strategy followed that is designed to simultaneously correct for both load factors, and which is analogous to that used for the same purpose in the muscle mechanics area. Specifically, ventricular pressure-velocity curves are initially determined and then these curves are extrapolated to zero pressure to yield maximum ventricular pressure, Vmax, which reflects contractile state independently of preload and afterload factors (see Mason et al., 1974).

Accordingly, regardless of whether contractility is viewed from a muscle mechanics or hemo­dynamic point of view, the major confounding factors of preload and afterload must be taken into account when any dependent measure is proposed as an index of contractility, and corrective steps must be taken. Since carotid dP/dt is a hemodynamically-based measure, the various hemo­dynamic corrections suggested above could be employed to obtain a more true indication of con­tractile changes in the myocardium. Since Obrist and his colleagues (Obrist et al., 1972, 1974, 1978) have not used such corrective measures the possibil­ity of confounding by loading factors is a real one. It is because of these loading factors that it has been asserted that k 4dP/dt per se has been found to be of limited value as an independent measure of myocardial contractility" (Mason et al., 1971, p. 48). It bears emphasis that such confounding from loading factors is not an empirically negligible source of difficulty. For example, it is known that raising the leg while lying down results in HR acceleration and elevates peak dP/dt. The latter effect, however, is a result of an augmentation of ventricular preload without any change in ven­tricular contractility (Mason, Sonnenblick, Ross, Covell, & Braunwald, 1965; Mason, Sonnenblick, Covell, Ross, & Braunwald, 1967).

In the figure, however, we have not included these various corrected intraventricular indices be­cause the indirect psychophysiological ventricular index of interest—carotid dP/dt—is based on the simple form of ventricular pressure which is uncorrected for, and therefore confounded by, the factors of preload and afterload. The indirect carotid dP/dt index, which will be the focus of the next two sub­sections, is therefore seen to have at least two problems to surmount before it can be considered to be a valid measure of myocardial sympathetic influ­ences. Those two problems are, as shown in Fig. 1 and as detailed above, the confounding influences due to the parasympathetic influences on contractil­ity and the influences of loading factors. However, there may be conditions where not only the effects of parasympathetic, but also those of loading factors are empirically negligible. This possibility leads, in

the next subsection, to an examination of the third inferential step in Fig. 1, a step involving the estima­tion of intraventricular dP/dt from aortic, and finally carotid, dP/dt.



Attempts to Validate Carotid dP/dt: Correlational Approach

Assuming (contrary to the above) that intraven­tricular dP/dt is a valid measure of contractility (and of sympathetic influences), it is reasonable to use the potentially noninvasive and unobtrusively mea­sured carotid dP/dt as a psychophysiological index of sympathetic influences. Still there is need for caution if only because, as indicated in Fig. 1, there are two structural links between the two dP/dt measures, i.e., the link between the ventricles and the aorta, and that between the aorta and the carotid artery. More importantly, the validation procedure needs to include an assessment of both the "candi­date" index (here, carotid dP/dt) and the criterion measure (here, intraventricular dP/dt). As Obrist et al. (1972) have indicated, the proposal to use carotid dP/dt as an index of intraventricular dP/dt had already been put forward by Rusher (1964). How­ever, Rushmer's proposal was put forward very cautiously and also included a validation procedure for checking on the proposal, a procedure that Obrist and his associates appear not to have fol­lowed. Specifically, the suggestion was that:

if the wave form of the arterial pulse wave recorded is not too greatlv deformed in its passage to the carotid arteries, a pulse wave recorded from within the carotid artery, or even by an external capsule, may have an initial slope that could be cor­related with simultaneously recorded direct measures of ven­tricular impulse. If the initial arterial pressure upslope can be established as a valid indicator of the rate of pressure rise and the rate of ejection into the aorta, a simple recording capsule with a differentiating circuit may have value as a tool ancillary to electrocardiograms in cardiology laboratories. (Rushmer, 1964. p. 279)

Rushmer's clear formulation appears to state a standard validational procedure: examining the correlation between the direct (here, the invasive intraventricular dP/dt) measure with the indirect (here, either invasive or noninvasive carotid pres­sure pulse wave dP/dt) measure.

Obrist and his associates have not been in a position to provide validating evidence of the form outlined by Rushmer because they have never re­ported the critical component of the validational correlation, i.e., the component of intraventricular dP/dt. It is only with that critical component that it is possible to produce validating evidence in Rush­mer's terms, evidence that shows high positive correlations between the carotid pulse-pressure

wave dP/dt and intraventricular dP/dt.1 Indeed, in their two most recent studies (Obrist et al.. 1974. 1978). no correlational data between their pulse-pressure dP/dt and any other measure (direct or indirect) are offered, perhaps because they intended their initial experiment (Obrist et al., 1972) to serve as validation of their carotid dP/dt measure. How­ever, even in that initial study the hemodynamically-based carotid dP/dt was not correlated di­rectly with intraventricular dP/dt as the criterion measure but with the rate of shortening of the ventricular muscles (Vmc).2 Correlating a hemodynamically-based candidate measure (i.e.. caro­tid dP/dt) with a criterion measure based on muscle mechanics (Vmc) is questionable because, even though both are aspects of ventricular func­tion, there is no quantitative equivalence between Vmc and intraventricular dP/dt (Falsetto. Mates. Greene. & Funnel. 1971). In any case, if a switch to the muscle-mechanics aspect were to be made in choosing the criterion measure against which to validate carotid dP/dt. the preferred measure would be Vmax. which controls for preload and afterload problems. However, even if the Vmc version of this muscular contraction rate measure is accepted as the criterion against which the indirect carotid dP/dt can be validated, the data which allow comparison of these two measures presented by these investigators (Obrist et al., 1972, Figs. 2 and 3) indicate that under critical conditions (i.e., the presentation of the US) the two measures behave in a markedly different manner. Therefore, there is no evidence to demonstrate the necessary condition of a set of high positive correlations between the carotid dP/dt mea­sure and either intraventricular dP/dt or Vmc.

The possibility of obtaining such a set of interpretable correlations has been further diminished by Obrist et al. 's (1978) most recent redefinition of the carotid dP/dt measure. This redefinition involves a ratio transformation which we have elsewhere indi­cated (Furedy & Heslegrave. 1979) to be contrary to both sound biological and measurement principles. The ratio transformation (for details see Obrist et al., 1978, p. 104. footnote) involves using the pre­viously used maximum slope of the ascending limb of the pulse wave as the numerator, but adding, as the denominator term, the maximum slope of the ascending component of the descending limb fol-

1 Indeed, even such validating evidence as high positive correlations may be difficult to obtain since as the pulse wave moves toward the periphery, the influence of reflected waves becomes greater (see McDonald, 1974).

2 Vmc, in the text above and in Fig. I, has been used to denote the rate of muscle shortening. Obrist et al. (N72) use the hemodynamic term, dP/dt, to denote this muscle shortening measure, but we suggest that a muscle-mechanics term such as Vmc is more appropriate

lowing the dicrotic notch. The biological difficulty with this transformation is that this denominator is affected by many noncontractile factors such as total peripheral resistance, the site of recording, reflected waves, and aortic resistance; the measurement diffi­culty is that it is not clear how the validational difficulties inherent in the numerator term (detailed above) are overcome rather than merely obscured by the introduction of a denominator term which itself is confounded by noncontractile factors.

The question may be raised as to why, in view of these difficulties, the ascending/descending (A/D) components ratio transformation was adopted in the first place. The answer seems to lie in the fact that the recording of the A component (i.e., the slope of the pulse wave's ascending limb) is subject to a great deal of artifactual influences, and it is the magnitude of these artifacts that the ratio transfor­mation was meant to reduce. Consistent with this interpretation of the rationale behind the ratio trans­formation is the claim that the w "ratio was used because it has been observed that it remains rea­sonably constant when the absolute amplitude of the pulse wave changes artifactually" (Obrist et al.. 1978, p. 104). The trouble with this rationale, how­ever, is that it is not clear why constancy of the ratio measure should be accepted as evidence that it has "solved" the problem of artifacts. On the contrary, if it is assumed (as is the case) that the A component contains artifacts, then the fact that the ratio trans­formation remains constant while the A component varies would seem to indicate that this constancy is achieved through opposing artifactual influences on the D component. It is questionable to claim that two artifactual changes can produce a true estimate when combined in a ratio formula. Another possi­bility is that constancy is achieved through an in­crease in error associated with the D component, an increase which would probably attenuate the sen­sitivity of the overall ratio index. In either case the very least that would need to be done would be to provide some systematic data relevant to these con­cerns. As it stands, then, this recent redefinition of carotid dP/dt by Obrist et al. (1978) would seem to increase rather than decrease the validational prob­lems inherent in the measure.

To summarize our arguments up to this point, Fig. 1 shows the direct validational technique neces­sary to infer sympathetic myocardial influences from changes in carotid dP/dt. With respect to the evidence above on the validity of the major assump­tions or links necessary to make this inference (shown in the figure), the conclusion that must be drawn is that carotid dP/dt has not been shown to validly index sympathetic myocardial activity. The basis for this conclusion is: a) parasympathetic activity has been shown to influence contractility, b)

preload and afterload factors contribute signifi­cantly to changes in intraventricular dP/dt, c) Obrist et al. have never monitored intraventricular dP/dt as a criterion measure, and d) the only comparison of validity has been between carotid dP/dt and Vmc (which is not equivalent to intraventricular dP/dt), and this comparison revealed markedly different patterns during critical (US presentations) events.

Attempts to Validate Carotid dP/dt: Pharmacological Blockade Approach

In principle there are ways of validating an index other than that of correlating it with a criterion and understanding the causal factors involved in that correlation. In the case of this particular index, the correlational approach is not particularly attractive not only because of the problems of having to link carotid dP/dt through aortic dP/dt to intraventricular dP/dt, but also because the connection between intraventricular dP/dt and sympathetic influences may be confounded by such factors as preload and afterload, as well as parasympathetic influences. Accordingly, it makes sense to attempt a more "empirical" validational procedure where inde­pendent experimental manipulations show that carotid dP/dt does, in fact, uniquely reflect sympa­thetic activity.

In line with this rationale, Obrist and his as­sociates have focused most of their efforts not on correlational methods (except for those used in their 1972 study reviewed above) but on the method of pharmacological blockade. In their use of the blockade methodology to validate their form of the carotid dP/dt measure, the procedure has been to first show that an experimental manipulation pro­duces an increase in carotid dP/dt in subjects with an intact ANS, and then observe whether a beta-adrenergic blocker attenuates that increase. Such attenuation results are then interpreted as evidence for validating their carotid dP/dt measure as an index of myocardial sympathetic activity. One rea­son for the attractiveness of this scheme is that, if it works, it virtually eliminates having to consider the carotid dP/dt measure as an "indirect" measure of intraventricular dP/dt. If a sound blockade methodology could yield consistent and favorable results, then it would be possible to treat the (unob­trusively measured) carotid dP/dt as a direct and valid index of myocardial sympathetic activity.

Unfortunately the results, when critically exam­ined, are neither internally consistent nor favorable to this view of carotid dP/dt. Before turning to these psychophysiological data, it is necessary to recog­nize that in addition to the loading and parasym­pathetic nervous system confounding possibilities that are potentially present in any use of carotid dP/dt as a sympathetic index (see Fig. 1 and discus-

sion above), the blockade methodology also intro­duces another sort of parasympathetic confounding influence that can be physiologically significant and which may be termed "compensatory parasym­pathetic confounding.'' This source of confounding arises from the fact that the ANS is an interactive, nonadditive system organized in such a way that parasympathetic influences are moderated by sym­pathetic tone (Levy, 1971, 1977). The blockade methodology, then, does not allow the investigator to observe the "true" interactive effects of the two ANS branches in their control over myocardial performance. This interactive, nonadditive organi­zation of the two ANS branches is evident even in ventricular contractility. As stated in the section labelled "Parasympathetic influences on ventricu­lar contractility," there is evidence showing that as sympathetic tone increases, antagonistic parasym­pathetic (usually negative inotropic) effects are en­hanced. If this argument were taken to the extreme case involving pharmacological blockade of the sympathetic system, one might expect negligible parasympathetic effects. However, pharmacologi­cal blockade, by preventing such sympathetic-parasympathetic interactions, actually obscures the state of affairs present in the normal, unblockaded heart because blocking one system can lead to compensatory adjustments which augment the other system (Katcher, Solomon, Turner, Lo Lordo, Overmeir, & Rescorla, 1969; Schneiderman. Van-derCar, Yehle, Manning, Golden, & Schneider­man, 1969). Contrary to normal conditions where parasympathetic influences are known to alter with sympathetic tone and usually in an antagonistic manner, blocking the sympathetic system seems to augment the effects of the parasympathetic system. This extreme compensatory adjustment is one which is outside the realm of normal in vivo ad­justments and hence labelled ' 'compensatory parasympathetic confounding." The compensatory adjustment argument also suggests the conclusion that even apparently clear blockade-based dP/dt results may be equivocal in terms of the presence of parasympathetic influences. Thus in cases where dP/dt responses to experimental (stress) manipula­tions were grossly attenuated by a beta-adrenergic blockade, one could still not conclude that under these conditions the dP/dt was a valid sympathetic index because of the possible (masked) presence of compensatory parasympathetic influences.

Turning now to the actual psychophysiological data obtained by Obrist and his associates, it would appear that these results do not support carotid dP/dt as a sympathetic index. Rather, these data suggest that compensatory sources of parasympathetic con­founding or other confounding influences, such as loading factors and HR changes, were in operation

to affect the purportedly sympathetic dP/dt index. One set of data that support this suggestion is summarized in Table 1 of Obrist et al. (1972), which shows the mean anticipatory and unconditional dP/dt responses in an aversive delayed-conditioning paradigm.3 The critical comparison of these mea­sures is between the normal and blockaded condi­tions, where the latter manipulation was designed to block the sympathetic nervous system. To the ex­tent that the dP/dt index is a unique and valid sympathetic indicator, it would be expected that the experimental conditions produce an increase in dP/dt during anticipatory and unconditional re­sponding in the intact condition, but not under beta-adrenergic blockade where any dP/dt respond­ing should be virtually eliminated. However, an inspection of the 18 mean changes from baseline during blockade (2 for each of the 9 dogs) in Table 1 (Obrist et al., 1972, p. 253) indicates that this index does not solely reflect sympathetic influences dur­ing the study; if this index were uniquely sympa­thetic, then the experimental conditions should have no significant effect on dP/dt when the sympathetic system was blockaded. In fact, examining the 18 blockaded dP/dt scores, one observes that contrac­tility significantly increased from baseline in 9 instances and significantly decreased from baseline in 2 instances. These significant changes contradict the expected effect of the beta-adrenergic blockade. From these significant results it seems clear that dP/dt reflects more than beta-adrenergic influences and probably involves both parasympathetic and loading influences. Since HR increased signifi­cantly in all dogs under both intact and blockaded conditions, the dominance of parasympathetic in­fluences on HR seems established. The form of this parasympathetic activity, however, is of the ex­treme compensatory sort occurring under blockade. Therefore, as noted above, the inotropic action of such parasympathetic activity cannot easily be es­tablished. Nevertheless, it would seem that a de­crease in compensatory parasympathetic tone, rela­tive to baseline, is responsible for the increase in HR. Since contractility changes have been shown to be inversely related to parasympathetic activity in most instances, it is also possible that this same decrease in parasympathetic tone from baseline could account for the relative increase in contractil­ity from baseline.

The possibility of inotropic changes being related to parasympathetic changes does not preclude the



3Note that all dogs are considered here since contractility estimates are provided for all dogs. It should be remembered, however, that there are two sorts of contractility estimates, i.e., Vmc (G dogs) and dP/dt (F and P dogs), which are not strictly comparable as noted above.

possibility that loading factors could also be in­volved. Data are only provided for afterload as­sessment through diastolic pressure changes. Here Obrist et al. use these somewhat inconsistent data to try to show that contractility is not affected by changes in afterload. It is true that the changes in afterload do not explain the dP/dt changes, but dP/dt could be modified by preload changes. Some (though not all) of their afterload effects provide support for the involvement of loading factors. Since a lack of change in afterload, as measured in the aorta, does not have explanatory power, we will only consider significant changes in afterload. In 4 dogs there were significant changes from baseline in afterload but these changes were not always re­flected in dP/dt. Specifically, a significant increase in afterload could be expected to yield a significant decrease in slope, which could explain the anticipa­tion effects in dog P-5. Conversely, a significant decrease in afterload could be expected to yield a significant increase in slope. In dogs G-l and P-7, under blockade, no change in slope occurred despite a significant decrease in afterload. More impor­tantly, for both P-6 and P-7 dogs, similar decreases in afterload during the UCR occurred for intact and blockaded conditions. In terms of dP/dt, however, each dog showed a significantly greater increase in dP/dt under intact conditions. Also, the P-6 dog showed a significantly greater increase in dP/dt than dog P-7 under blockade. Finally, a significantly greater reduction in afterload from intact to block­ade conditions for dog G-l resulted in a signifi­cantly greater reduction in dP/dt for that dog. A plausible interpretation of these significant loading differences and the corresponding dP/dt differences, as compared to baseline, is that the usual and known effects of afterload changes are not in operation and other compensations, such as alterations in preload, are occurring. This interpretation is at odds with that of Obrist et al. (1972) which is that loading factors are not influential. Until such interpretations as ours are clearly ruled out, the results of Obrist and his associates lend no more than equivocal support to the contention that carotid dP/dt is a valid measure of beta-adrenergic influences on the myocardium. Similarly, the second study of Obrist et al. (1974) reveals dP/dt effects that are not clearly interpretable. These relevant data are those presented in their Figs. 2 and 3 which show the dP/dt (their Fig. 2) and HR (their Fig. 3) in a stressful reaction-time exper­iment under intact and pharmacologically block­aded conditions. Following the "Ready "and "Re­spond" signals, changes in dP/dt in the intact sub­jects are indistinguishable from those in the (beta-adrenergically) blockaded subjects. These data not only suggest that dP/dt is influenced by other (load­ing) factors but also serve to illustrate a case where

dP/dt seems to be primarily under the control of non-sympathetic influences, since the sympathetic blockade manipulation had no effect.

Another aspect of these results seems, on closer examination, to be inconsistent with a solely sympa­thetic interpretation of the dP/dt index. In the words of the investigators themselves, "carotid slope and HR were more or less 180 degrees out of phase with each other" (Obrist et al., 1974, p. 413). These data raise two issues. The first, more general issue is that Obrist et al. imply that if carotid dP/dt and HR were not out of phase with each other, the results would favor dP/dt as a valid measure. In other words, parallel changes in dP/dt and HR during sympa­thetic blockade would support dP/dt as a sympa­thetic index. Such an interpretation is contrary to the work of other investigators (e.g.. Mason. 1969). Mason (1969) noted that without control over hemodynamic variables, it is only possible to inter­pret directional changes in contractility when peak dP/dt and HR responses are in opposite directions. Therefore, even if dP/dt and HR had not been out of phase with each other, the confounding of dP/dt by alterations in HR would have prevented an interpre­tation positively supporting dP/dt as an index of sympathetic activity.

The second, more specific issue concerns the actual interpretation of these data. Obrist et ah interpret the differences between intact and block­aded subjects on both HR and peak dP/dt as repre­senting sympathetic influences. Let us consider first the HR data. Prior to shock occurrence the lack of difference between intact and blockaded subjects seems readily attributable to parasympathetic influ­ences. Following shock occurrence, the difference between the intact and blockaded subjects seems to reveal a faster recovery profile for the parasym­pathetic system which thereby unmasks the sympa­thetic effects following shock occurrence. This in­terpretation seems reasonable since even moderate parasympathetic activity is capable of masking strong sympathetic effects (Levy & Zieske, 1969b). Now let us consider whether a similar argument can be applied to the peak dP/dt data in view of the various hemodynamic confounding influences noted earlier. Obrist et al. suggest that diastolic pressure changes affect the peak dP/dt in the initial part of the trial. Such a suggestion is, in part, probably correct given that the reported increase in diastolic pressure, and thus afterload, is probably mediated through alpha-adrenergic vasoconstriction and thus would be unaffected by beta-adrenergic blocking agents. This effect would ex­plain the decrease in dP/dt early in the trial. The subsequent increase in dP/dt, however, does not seem to be readily explainable by afterload changes and is probably more explicable by homeometric

autoregulation (Sarnoff & Mitchell, 1961). How­ever, the question arises as to whether these intrinsic influences provide as plausible an explanation for the eventual separation between intact and block­aded subjects as the interpretation of sympathetic influences. During the differentiation of the groups. Obrist et al. report a paradoxical pattern of results comprising a pronounced sympathetic effect in HR (and peak dP/dt) accompanied by no change in peripheral diastolic blood pressure. They attributed this pattern to a pronounced vasodilator effect in the striate musculature of the intact subjects. Given this interpretation, an alternative explanation for the changes in peak dP/dt is provided. This vasodilatation may have resulted in a dramatic decrease in aortic pressure and hence an increase in dP/dt. Since the vasodilation cannot occur in the blockaded subjects, it may account for the differences in the data of the two groups. Although it is possible in this case that dP/dt reflects sympathetic effects, it would seem as likely that these effects do not reflect contractility or myocardial sympathetic effects at all but rather reflect beta-adrenergic vasodilatory effects.




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