A. Phosphorous (Kα) X-ray map. B. Calcium (Kα) X-ray map. Note the gap between the canal wall and the Cal socket. C. rows top to bottom – raw:selected areas of Ca, P. PC 1: Calcite PC used to choose calcite pixels. PC 2: Wall PC used to choose wall pixels. D. Calcite and Wall pixels are plotted showing their Ca/P ratios relative to clam calcite (green), HAP (red) and mono-calcium phosphate (blue). The brightness of images A,B and C-raw scales linearly with X-ray intensity, and therefore approximately with concentration which are plotted to produce ratios in D. A 50 um calibration bar is seen in panel A.
Figure 6. Primary, secondary and tertiary organule cuticle structures on the dorsal carapace of an intermolt lobster. Three size and complexity levels for organules are presented pointed to by increasing sized arrows. The first level is a simple gland tube opening, the second combines a bristle and a gland opening and the third level has more organule components.
