5 – 26. Department of Transportation categories of drug and alcohol testing a. Civilian employees to whom DOT regulations apply are subject to testing under circumstances described in 49 CFR Part 382, Subpart C. These include the following six bases for drug testing pre-employment drug testing only post-accident testing reasonable suspicion testing random testing followup testing and return-to-duty testing. While similar to the DFW drug testing categories listed in paragraph 5 – 6, DOT categories have different requirements see DA Pam 600 – 85 for more information. b. The applicability of drug testing requirements under the DOT regulations does not exempt an employee from the requirements for testing under the auspices of the DHHS DFW regulations when applicable.