Serology: Enzyme-linked immunosorbent assay (elisa) Authors
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Figure 1: Diagrammatic representation of a blocking ELISA. Y = antibody in test serum. e-Y = detector antibody conjugated to a marker enzyme. A competitive ELISA works on the same principle but in this case the test serum and the competing antibody (MAb or polyclonal serum specific for the antigen) are added as a mixture to the wells on the plate. They compete for binding sites on the antigen. In the simplest versions of the test the competing antibody is conjugated to a marker enzyme ( ). The plate is then washed and substrate added in the normal way. The percentage of inhibition of colour formation is calculated. 
Figure 2: Diagrammatic representation of a competitive ELISA Directory: sites -> default -> files files -> Answer True False 2 points Question 2 files -> Northern England’s set-jetting locations files -> Nstructions for Acquiring Excess Equipment online, through the 1033 Program files -> Occupational health and safety files -> The Black Panther Party’s Ten Point Program files -> International programs roel profile files -> Fermi Questions a guide for Teachers, Students, and Event Supervisors Lloyd Abrams, Ph. D. DuPont Company, cr&D/ccas experimental Station Wilmington, de 19880 files -> Personal Information Name: Maha Al-Ammari Nationality: Saudi Relationship Status Download 322.66 Kb. Share with your friends:
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