The Haemophilus somnus
129Pt genome encoded genes involved in the utilization of glucose, ribose, xylose, fucose, galactitol, glucitol/sorbitol, mannose and mannitol (Table S5), while H. ducreyi
35000HP had genes involved in glucose and mannose utilization. The H. influenzae
Rd genome contained genes involved in the utilization of glucose, ribose, xylose, fucose, glycerol, fructose and galactose (15, 27).
The most interesting aspect of carbohydrate utilization involved the genes for glucose and fructose transport. H. influenzae Rd has the enzyme I (ptsI, HI1712), Hpr (ptsH, HI1713) and glucose specific IIA (crr, HI1711) genes for the glucose phosphotransferase system (PTS), but not the gene encoding membrane bound glucose-specific enzyme II (ptsG) (Fleischmann et al, 1995). Both H. somnus 129Pt and H. ducreyi 350HP had the ptsI (HS_1097, HD0228), ptsH (HS_1098, HD0229) and crr (IIAGlc, HS_1096, HD0227) genes and, like H. influenzae, lacked ptsG.
We found that H. somnus
129Pt and H. ducreyi
35000HP, like H. influenzae
Rd (15), lacked genes encoding the membrane-bound glucose specific enzyme IIBC (ptsG)
of the glucose PTS, indicating that they did not have a functional glucose PTS for glucose transport. Therefore, instead of being involved in glucose utilization, the product of the crr
gene may be involved in regulation of carbon metabolism and stress response in these Haemophilus
strains, as has been proposed for S. coelicolor
(22) and P. multocida
(4). If this is true, how do these organisms take up glucose? A previous study of H. influenzae
suggests that it takes up glucose via a non-PTS permease (26). We did find one gene in H. somnus
129Pt that encoded a sodium/glucose symporter (HS_0338). However, this gene was not present in H. influenzae
Rd or H. ducreyi
35000HP. Glucose may enter these organisms via an unidentified permease, an ABC transport system or may be transported by a permease that is specific to another sugar (26). Another option for H. ducreyi
35000HP and H. somnus
is that they may be able to transport glucose via a mannose PTS, as has been described for P. multocida
(4), since they both had genes encoding the mannose PTS. Further evidence in support of this option was our finding that H. ducreyi
35000HP had no genes with sequence similarity to known glucokinase genes. However, it is possible that H. ducreyi
35000HP contains a functional homolog with no sequence similarity to other glucokinase/hexokinase genes. As an alternative to glycolysis, P. multocida
may use the pentose phosphate pathway to process glucose (4). This may also be the case for H. somnus
129Pt, H. ducreyi
35000HP and H. influenzae
Rd, which all have the complete set of pentose phosphate pathway genes.
had the fruBKA
operon (HI0446 – HI0448) encoding components of the fructose PTS
, consisting of the fruB
gene encoding the protein FPr, fruK
(1-phosphofructokinase), and fruA
, encoding the fructose-specific IIBC component. Neither H. somnus
129Pt nor H. ducreyi
35000HP had these genes. The closest relative to the fructose PTS is the mannitol PTS (35), which only H somnus
129Pt had. This is interesting in light of evidence that H. somnus
can ferment fructose in culture (16). It is possible H. somnus
takes up fructose via either a mannose or mannitol PTS
, which it encodes, or by an unidentified permease. Both H somnus
129Pt and H. influenzae
Rd had genes that were similar to the E. coli mak
gene (HS_1253; HI1082), which encodes cryptic manno(fructo)kinase that converts fructose to fructose-6-phosphate, as well as pfkA
(6-phosphofructokinase 1; HS_0485; HI0982) and fbaA
(fructose bisphosphate aldolase class II; HS_0206; HI0524), all of which are part of the fructose degradation pathway. H. ducreyi
35000HP had homologs of pfkA
(HD0465) and fbaA
(HD0864) but not mak
. H. somnus
129Pt also had a gene that encodes a possible fructose bisphosphate aldolase class I (HS_0055).
In H. influenzae, the galactose utilization genes galMKTR (HI0818 – HI0821) are located together, while galU (HI0812) and galE (HI0351) are not (28). Like H. influenzae Rd, H. somnus 129Pt had the genes galK, galM, galU and galE, but only galK (HS_0235) and galM (HS_0236) were located next to each other (Table S5). It did not have galT, which encodes galactose-1-phosphate uridylyltransferase or galR, the galactose operon repressor. H. ducreyi had only galU (HD1431) and galE (HD0829). GalE is required for the biosynthesis of extracellular polysaccharide materials such as lipopolysaccharide (LPS) and capsule (36). galU is an essential virulence gene that is critical in generating sugar precursors needed for polysaccharide formation and LOS outer core synthesis (49). The unlinked location of galE relative to the other gal operon genes in H. somnus 129Pt and H. influenzae Rd is also seen in Pasteurella (Mannheimia) haemolytica A1; the separation of galE from the rest of the gal operon may have resulted from a transposition event (36). P. haemolytica A1 is normally found inside its bovine host, and therefore may not need a catabolic gal operon, since it probably does not have to grow on galactose as its sole source of carbon (36). Since they are missing some or many of the gal operon genes, and can use other sugars, this may also be the case for H. somnus 129Pt.
Both H. somnus 129Pt and H. influenzae Rd had the ribose operon genes. The H. influenzae rbs genes were organized in an operon, in the same order as in E. coli (3). In contrast, the H. somnus 129Pt rbs operon genes were present in 4 locations around the genome: 1) rbsD, rbsK and rbsR (HS_0223 – HS_0227), HS_0224 and HS_0224a were possible transposases; 2) rbsACB (HS_0763-HS_0765); 3) and 4) H. somnus 129Pt had 1 extra copy of rbsA (HS_0768) and 2 extra copies of rbsC (HS_0769 and HS_1580).
H. somnus 129Pt and H. influenzae Rd both had genes involved in D-xylose and xylitol degradation (Table S5). The genes for D-xylose degradation to D-xylulose 5-phosphate, xylA (HS_0587, HI1112) and xylB (HS_0588, HI1113), were not present in H. ducreyi 35000HP. H. somnus 129Pt and H. influenzae Rd also had most of the components of the xylitol degradation pathway, which converts xylitol to xylulose 5-phosphate. The first step in this pathway, the conversion of xylitol to L-xylulose, is catalyzed by a xylitol 4-dehydrogenase/L-xylulose reductase, which exists in Erwinia uredovora (10) and many eukaryotes. However, there were no gene or protein sequences available from E. uredovora to use in blast searches. Blastx of the L-xylulose reductase gene sequence from Trichoderma reesei (Hypocrea jecorina) hit reductase/dehydrogenase proteins in H. somnus 129Pt (HS_0167), H. ducreyi 35000HP (HD0708) and H. influenzae Rd (HI0155) at 29% – 30% amino acid identity. So, the xylitol 4-dehydrogenase activity may be present in these organisms. The next reaction, conversion of L-xylulose to L-xylulose 5-phosphate is catalyzed by L-xylulokinase (9), which was present in H. somnus 129Pt (HS_0770) and H. influenzae Rd (HI1027), but not in H. ducreyi 35000HP. However, H. somnus 129Pt, H. ducreyi 35000HP and H. influenzae Rd all had rpe (HS_0175, HS_0057, HD1929, HI0566), encoding ribulose phosphate 3-epimerase, which converts D- and L-xylulose 5-phosphate to D- or L-ribulose 5-phosphate (42). There was a cluster of genes in H. somnus 129Pt (HS_0763 to HS_0773) involved in ribose and xylitol metabolism. Although H. influenzae Rd had all of these genes, they were not organized on the chromosome in the same way. As mentioned above, H. influenzae Rd had only 1 copy of the rbs operon (HI0501 – HI0506), which was not adjacent to the sgbK/lyx, sgbH, sgbU and araD genes (HI1024 – HI1027), as one set of rbs genes was in H. somnus 129Pt.
Like H. influenzae Rd, H. somnus 129Pt had the fuc operon (HS_1446 – HS_1451; HI0610 – HI0615), so it can probably metabolize fucose. H. ducreyi 35000HP did not have these genes. Both H. influenzae Rd and H. somnus 129Pt had an extra copy of the fucA gene (HS_0014, HI1012), which encodes fucose-1-P aldolase. Both H. somnus 129Pt and H. ducreyi 35000HP had the mannose utilization genes manAZYX (HS_0605 – HS_0609; HD0765 – HD0768), while H. influenzae Rd did not. However, the genes that flanked the mannose utilization genes in H. somnus 129Pt and H. ducreyi 35000HP were different. H. ducreyi 35000HP had a complete set of genes encoding the mannose PTS and mannose 6-phosphate isomerase (HD0765-HD0768).
129Pt had the galactitol utilization operon (HS_1140 – HS_1146), but H. influenzae
Rd and H. ducreyi
35000HP did not. H. influenzae
Rd and H. ducreyi
35000HP did have the pflA
genes that flanked the galactitol operon in H. somnus
129Pt, although they were on the opposite strand in reverse order. H. somnus
129Pt had the glucitol/sorbitol utilization operon (HS_0675 – HS_0679) flanked by genes purE
(opposite strand HS_0672) and pepP
(HS_0682), H. ducreyi
35000HP had the flanking genes on opposite strands with other genes in-between (HD1419 – HD1423; aspC
, hypothetical, purK
). H. influenzae
Rd had purE
in a row (HI1615 – HI1617), but pepP
(HI0816) was located approximately 800 genes away.
129Pt had a mannitol utilization operon consisting of mtlADR
(HS_1250 – HS_1252). This was like the mtl
operon in E. coli,
consists of the mtlA
, and mtlD
genes that encode the mannitol transporter (enzyme IICBAmtl), a transcriptional regulator, and mannitol-1-phosphate dehydrogenase (46). H. ducreyi
35000HP and H.influenzae
Rd did not have these genes. HD1859 may be a mannitol/fructose specific IIA component of a PTS, but H. somnus
129Pt did not have it.
129Pt had a gene that was similar to E. coli celB
(HS_0437), which encodes a cellobiose-specific IIC component
, but did not have genes encoding the complete cellobiose PTS. H. ducreyi
35000HP and H. influenzae
Rd did not have any cellobiose PTS genes.
Because H. somnus in culture has been reported to use trehalose and maltose (16), we looked for genes involved in trehalose and maltose uptake and degradation. The enzyme II of the trehalose PTS (encoded by treB) can function with the rest of the glucose PTS (crr, HPr, EI) (1, 34). However, H. somnus 129Pt, H. ducreyi 35000HP and H. influenzae Rd did not have treB. None of the organisms had treC, enoding the trehalose 6-P hydrolase or treA, encoding the periplasmic trehalase. So trehalose probably does not enter the cell via the PTS or follow the trehalose I degradation pathway. The genomes of H. somnus 129Pt, H. ducreyi 35000HP and H. influenzae Rd also didn’t have a gene encoding trehalose-6-phosphate phosphorylase (trePP) or treF, encoding cytoplasmic trehalase, which is part of the trehalose degradation II pathway. Trehalose can also enter cells via a permease (34), followed by trehalose phosphorylase conversion of trehalose to glucose-1-P. However, none of these organisms had genes encoding trehalose phosphorylase. With regard to maltose, H. influenzae Rd, H. somnus 129Pt and H. ducreyi 35000HP were missing all of the key E. coli genes involved in maltose uptake and degradation (malT, malS, malE, malF, malG, malK, malP, malZ) (5). H. somnus 129Pt and H. influenzae Rd did have malQ, which is necessary for maltose metabolism in E. coli (5).