2008 Annual Meeting fhs-afs abstract Submission and Registration Announcement
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- Courier New font ; 3. Capitalize every word in the title and copy put this in the “ Title box
- NB: Abstract should be limited to 300 words or less ! Full Presentations and Posters ( 1 m 2 ) will also be submitted on-line between May 16 st
- Title box “ IMPROVED DIAGNOSTIC TECHNOLOGIES FOR EARLY TISSUE CULTURE DETECTION OF BETATNODVIRUS IN ATLANTIC COD Gadus morhua “ Abstract box
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Abstract Instructions: 1. Abstracts for Posters and Oral Presentations will be submitted on-line through the official conference website at www.upei.ca/FHS-AFS2008; 2. The primary author must fill in “ Author ” details, but secondary authors do not have to be added to this section of the on-line submission; 2. Titles, authors, affiliations and abstracts can be cut and pasted into the website directly from word processing packages or typed into the site manually. Use Courier New font; 3. Capitalize every word in the title and copy put this in the “ Title box “ of the on-line submission; 4. Denote genus and species with italic script; 5. Place authors, affiliations and abstract all into the on-line submission “ Abstract box “; 5. For authors, use first and last name of each author. Underline the name of the author who will give the presentation, even if that individual is the sole author. Follow each author with a numbered affiliation; eg (1), (2) etc; 6. Provide a single-blank space between the authors and each affiliation. Affiliations should be a complete mailing address including zip code. The primary author’s affiliation should contain their email address 7. Provide a double blank space between the affiliations and the body of the abstract; 8. The abstract should be a single paragraph. There should not be any tables, figures, or citations within the abstract. NB: Abstract should be limited to 300 words or less ! Full Presentations and Posters ( 1 m2 ) will also be submitted on-line between May 16st and June 20th and should be prepared in POWERPOINT. Presentations will be limited to 15 minutes, including Questions and Answers! EXAMPLE ABSTRACT: ***************************************************************************** “ Title box “ IMPROVED DIAGNOSTIC TECHNOLOGIES FOR EARLY TISSUE CULTURE DETECTION OF BETATNODVIRUS IN ATLANTIC COD Gadus morhua “ Abstract box “ Tokinori Iwamoto (1), Carmencita Yason (2), David Groman (3) and Dorota Wydowska (4) 1 Department of Pathology & Microbiology, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, Prince Edward Island. C1A4P3. Canada tiwamoto@upei.ca 2 Regional Diagnostic Virology Unit, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, Prince Edward Island. C1A4P3. Canada 3 Aquatic Diagnostic Services, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, Prince Edward Island. C1A4P3. Canada 4 Electron Microscopy Unit, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, Prince Edward Island. C1A4P3. Canada Betanodavirus infections cause significant mortality in over 30 marine fin-fish species, with clinical disease generally occurring during early fry and fingerling stages. The ensuing disease has been termed VNN (viral nervous necrosis) or VER (viral encephalopathy and retinopathy). Currently, primary virus isolation for the ACNNV routinely occurs according to OIE protocols in the SSN-1 cell line, a mixed cell culture line containing a C-type retrovirus derived from Striped Snakehead. In low titre infections virus isolation and confirmation can take over 10 days. Work completed by Iwamoto and colleague in 2000 resulted in the development of a clone of the SSN-1 which they termed E-11. Subsequent work by these researchers in 2001 confirmed that detection of Betanodavirus could be improved using a combination of cultivation in the E-11 cell line followed by a RT-PCR assay after only 48 hours incubation. These protocols were applied as a means to more rapidly isolate and confirm ACNNV infection during routine diagnostic testing. We report here a description and characterization of C-type retrovirus harbored by the E-11 cell line, comparison of the sensitivity of SSN-1 and E-11 cell line for isolation of the ACNNV, provide an improved diagnostic protocol for isolation and confirmation of ACNNV and potentially all Betanodaviruses. **************************************************************************** FHS / AFS 2008 Annual Meeting Charlottetown, Prince Edward Island, Canada Phone: 902-566-0864 Fax: 902-566-0723 email: FHS2008@upei.ca 
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