Datum vypracování: 2004-10-21


Databáze universitních knihoven universit Oxford, Cambridge a Institutu neurologie university Londýn



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Databáze universitních knihoven universit Oxford, Cambridge a Institutu neurologie university Londýn.
1)


Personal name

Bergeron, Bryan P.

Title

Bioinformatics computing, Bryan Bergeron

Subject

Bioinformatics

Class number

570.285, BER

Publication

Upper Saddle River, N.J., London, Prentice Hall PTR, 2003

Format:

Books

2)


Title

Bioinformatics, sequence, structure and databanks - a practical approach, Ed. Des Higgins and W.Taylor

Subject

Bioinformatics

Class number

570.285, BIO

Publication

Oxford, Oxf.U.P., 2000

Series

Practical Approach

Format:

Books

3)


Personal name

Baldi, Pierre

Title

Bioinformatics, the machine learning approach, Pierre Baldi, Sřren Brunak

Subject

Bioinformatics

Class number

570.285, BAL

Publication

Cambridge, Mass., London, MIT Press, c1998

Series

Adaptive computation and machine learning

General note

"A Bradford book."

Format:

Books

4)


Personal name

Baldi, Pierre

Title

Bioinformatics, the machine learning approach, Pierre Baldi, Sřren Brunak

Subject

Bioinformatics

Class number

570.285, BAL

Edition

2nd ed

5)


Personal name

Attwood, T. K.

Title

Introduction to bioinformatics

Subject

Bioinformatics

Class number

570.285, ATT

Publication

Harlow, Longman, 1999

Format:

Books

6)


Publication

Cambridge, Mass., MIT Press, 2001

Series

Adaptive computation and machine learning

Edition history note

Previous ed.: 1998

General note

"A Bradford book"

Format:

Books

7)


Personal name

Krane, Dan E.

Title

Fundamental concepts of bioinformatics, Dan E. Krane and Michael L. Raymer

Subject

Bioinformatics

Class number

570.285, KRA

Publication

San Francisco, London, Benjamin Cummings, 2003

Format:

Books

8)


Title

Bioinformatics, managing scientific data, edited by Zoe Lacroix, Terence Critchlow

Subject

Bioinformatics

Class number

570.285, BIO

Publication

San Francisco, Calif., Morgan Kaufmann, Oxford, Elsevier Science, 2003

Format:

Books

9)


Title

Essentials of genomics and bioinformatics, edited by C.W. Sensen

Subject

Bioinformatics

Class number

570.285, ESS

Publication

Weinheim, Wiley-VCH, c2002

Edition history note

Concise ed. of Biotechnology, vol 5b. 2001

Added title

Biotechnology

Format:

Books

10)



Title

Bioinformatics, databases and systems, edited by Stanley Letovsky

Subject

Bioinformatics

Class number

570.285, BIO

Publication

Boston, London, Kluwer Academic, c1999

Format:

Books

11)



Main Entry  

Lesk, A,M.

Title  

Introduction to bioinformatics.

Imprint  

Oxford University Press. 2005.

12)



Dewey class mark  

574.1925

Title  

Chromatin and Chromatin remodelling enzymes. Part A / edited by C. David Allis, Carl Wu.

Imprint  

San Diego, CA ; London : Elsevier Academic Press, 2004.

Descr.  

xxxviii, 540p. :  ill. ;  24cm.

Series  

Methods in enzymology ; 375

Contents  

Contents: Histone bioinformatics ; Biochemistry of histones, nucleosomes, and chromatin ; Molecular cytology of chromatin functions.

Subject - Lib.Cong.  

Enzymes

 

Chromatin

 

Enzymology

Add.Entry  

Allis, C. David

 

Wu, Carl

 




holdings (1)  

All items

ISBN  

0121827798 (cased) : 99.95

13)


Dewey class mark  

574.0285

Title  

Bioinformatics and genomes : current perspectives / edited by Miguel A. Andrade

Imprint  

Wymondham : Horizon Scientific, 2003

Descr.  

xii, 227p ;  24cm

Subject - Lib.Cong.  

Genomes -- Data processing

 

Bioinformatics

Add.Entry  

Andrade, Miguel A.

 




holdings (1)  

All items

ISBN  

1898486476 (cased) : 80.00

14)



Dewey class mark  

574.0285

Title  

Bioinformatics and genomes : current perspectives / edited by Miguel A. Andrade

Imprint  

Wymondham : Horizon Scientific, 2003

Descr.  

xii, 227p ;  24cm

Subject - Lib.Cong.  

Genomes -- Data processing

 

Bioinformatics

Add.Entry  

Andrade, Miguel A.

 




holdings (1)  

All items

ISBN  

1898486476 (cased) : 80.00

15)



Main Entry  

Institute of Electrical and Electronics Engineers .

Title  

Transactions on nanobioscience

Imprint  

Institute of Electrical and Electronics Engineers

Subject - Lib.Cong.  

Ultrastructure (Biology)

 

Nanoscience

 

Bioinformatics

Add.Title  

IEEE transactions on nanobioscience

 




holdings (1)  

All items

holdings (2)  

Year 2004

holdings (2)  

Year 2003

ISSN  

1536-1241

16)



Dewey class mark  

574.870285

Title  

Bioinformatics : genes, proteins and computers / edited by Christine Orengo, David Jones, Janet Thornton

Imprint  

Oxford : BIOS, 2003

Descr.  

xiv, 298p ;  25cm

Subject - Lib.Cong.  

Molecular biology -- Computer simulation

 

Proteins -- Analysis -- Data processing

 

Genetics

Add.Entry  

Jones, David

 

Orengo, Christine

 

Thornton, Janet

 




holdings (1)  

All items

ISBN  

1859960545 (pbk) : 29.99

17)



Dewey class mark  

574.870285

Title  

Bioinformatics : genes, proteins and computers / edited by Christine Orengo, David Jones, Janet Thornton

Imprint  

Oxford : BIOS, 2003

Descr.  

xiv, 298p ;  25cm

Subject - Lib.Cong.  

Molecular biology -- Computer simulation

 

Proteins -- Analysis -- Data processing

 

Genetics

Add.Entry  

Jones, David

 

Orengo, Christine

 

Thornton, Janet

 




holdings (1)  

All items

ISBN  

1859960545 (pbk) : 29.99

18)



Dewey class mark  

574.0285

Main Entry  

Lesk, Arthur M.

Title  

Introduction to bioinformatics

Imprint  

Oxford : Oxford University Press, 2002

Descr.  

xvi, 283p :  ill ;  25cm

Subject - Lib.Cong.  

Bioinformatics

 




holdings (1)  

All items

ISBN  

0199251967 (pbk) : 19.99

19)



Dewey class mark  

574.0285

Main Entry  

Westhead, David R.

Title  

Bioinformatics / David R. Westhead, J. Howard Parish and Richard M. Twyman

Imprint  

Oxford : BIOS, 2002

Descr.  

viii, 257p :  ill ;  25cm

Series  

Instant notes

Bibliogr.  

Includes bibliographical references and index

Subject - Lib.Cong.  

Bioinformatics -- Examinations -- Study guides

Add.Entry  

Parish, J. H. (John Howard)

 

Twyman, Richard M.

Add.Title  

Instant notes in bioinformatics

 




holdings (1)  

All items

ISBN  

1859962726 (pbk) : 16.99

20)



Dewey class mark  

574.870285

Main Entry  

Baxevanis, Andreas D.

Title  

Bioinformatics : a practical guide to the analysis of genes and proteins / Andreas D. Baxevanis [and] B.F. Francis Ouellette

Edition  

2nd ed.

Imprint  

New York ; Chichester : Wiley, 2001

Descr.  

xviii, 470p

Series  

Methods of biochemical analysis ; 43

Gen. note  

Previous ed.: 1998

Subject - Lib.Cong.  

Molecular biology -- Computer simulation

 

Molecular biology -- Mathematical models

 

Proteins -- Analysis -- Data processing

 

Genetics

Add.Entry  

Ouellette, B. F. Francis

 




holdings (1)  

All items

ISBN  

0471383902 (cased)

 

0471383910 (pbk.) : 51.95

21)



Dewey class mark  

574.8702854

Title  

Bioinformatics : sequence, structure, and databanks : a practical approach / edited by D. Higgins and W. Taylor

Imprint  

Oxford : Oxford University Press, 2000

Descr.  

xx, 249 p. 1 v. (pbk) ;  24cm

Series  

The practical approach series

Subject - Lib.Cong.  

Biology -- Data processing

 

Biomolecules -- Data processing

Add.Entry  

Higgins, Des

 

Taylor, Willie

 




holdings (1)  

All items

ISBN  

0199637903 (pbk.) : 29.95

 

0199637911 (cased)

22)



Dewey class mark  

574.0285

Main Entry  

Attwood, Teresa K.

Title  

Introduction to bioinformatics / Teresa K. Attwood and David J. Parry-Smith

Imprint  

Harlow : Pearson Education, 1999

Descr.  

xxv,218p :  ill (pbk) ;  24cm

Series  

Cell and molecular biology in action series

Bibliogr.  

Includes index

Subject - Lib.Cong.  

Molecular biology -- Data processing

Add.Entry  

Parry-Smith, David J.

 




holdings (1)  

All items

ISBN  

0582327881 (pbk) : 23.99

23)



Bioinformatics : the analysis of protein sequence homology and protein struc






Mistry, T    Other titles by Author(s)

Publication Date: 2002

Control Number: M0018105KP

Copies:   1 copy - Show Copy

24)



Bioinformatics






Lacey, N    Other titles by Author(s)

Publication Date: 2003

Control Number: M0022613KP

Copies:   1 copy - Show Copy

25)



Bioinformatics : a practical guide to the analysis of genes and proteins / [. - 2nd ed






Publication Date: 2001

Control Number: 0471383902

26)



Title

Briefings in bioinformatics




Publisher

London : Henry Stewart




Control Number

1467-5463




Shelved at

journals



27)



Bioinformatics : sequence, structure, and databanks : a practical approach /






Publication Date: 2000

Control Number: 0199637911

28)



Bioinformatics : the machine learning approach / Pierre Baldi, Søren Brunak. - 2nd ed






Baldi, Pierre    Other titles by Author(s)

Publication Date: 2001

Control Number: 026202506x

29)


Developing bioinformatics computer skills / Cynthia Gibas, James Fenton and






Gibas, Cynthia    Other titles by Author(s)

Publication Date: 2000

Control Number: 1565926641

30)



Genetically yours : bioinforming, biopharming, biofarming / Hwa A. Lim






Lim, Hwa A.    Other titles by Author(s)

Publication Date: 2002

Control Number: 9810249381

31)



Molecular biology of the gene / James Watson ... [et al.]. - 5th ed






Publication Date: 2004

Control Number: 0321223683

32)



Molecular biology of the gene / James Watson ... [et al.]. - 5th ed






CD-ROM

Publication Date: 2004

Control Number: M0025595KP

33)


1. Proteomics Techniques •••
Royal Free Campus Departments Home About us Facilities Microscopy Techniques Administration Costs...
  ...Bioinformatics Sample Preparation Optimal sample preparation is essential for good 2D results. The ideal process will cause complete solubilization,...
  ...Bioinformatics Data from the mass spectrometer consists of a series of molecular ion m/z (mass/charge) values that are translated into to a...
60% Tue, 07 Sep 2004 10:08:56 GMT http://www.rfc.ucl.ac.uk/departments/Biomedical-...
Introduction
This facility has been set up to help researchers establish and run proteomic projects and identify biological molecules of interest – particularly proteins.

The facility is currently equipped with Amersham Biosciences 2D gel electrophoresis equipment, a Waters/Micromass capillary HPLC system coupled to a qToF-µ electrospray mass spectrometer.

2D gel electrophoresis is a suitable technique for asking, “Where do differences arise amongst the proteins in two similar samples?” For example, closely matched samples from diseased and healthy cells can be compared. Differences in protein abundance or covalent modification (e.g. phosphorylation, glycosylation and acylation) can provide important clues to the pathogenesis, progress and treatment of a disease.

Once a protein has been isolated and digested, the mass spectrometer is a suitable tool for asking, “What is this protein?”, “Which residues are modified?”, and “What is the modification?”



As there are many practical considerations in setting up these types of experiments it is strongly advised that you contact the facility staff about the design of the study prior to the preparation of samples. The following guidelines provide a brief introduction for those interested in proteomics:

Sample Preparation                                          
Optimal sample preparation is essential for good 2D results. The ideal process will cause complete solubilization, disaggregation and denaturation of the proteins in the sample. However, as samples vary in their constituent properties, optimal procedures must be determined empirically for each type of sample.
The development of the sample preparation must take into account the object of the investigation: increasing the range and number of detectable proteins can sometimes only be obtained at the expense of clarity and reproducibility. Close collaboration between investigators and facility staff should produce a sample preparation protocol tailored to the needs of the investigation.

Protecting Samples against Proteolysis
Disrupting tissue or cultured cells can liberate or activate endogenous proteases. As proteolytic degradation of proteins greatly complicates 2D electrophoresis, measures should be included to avoid this problem. Proteases can be inactivated by immediate snap-freezing or denaturing samples for example with 10% TCA, 8M urea or 2% SDS. However, the addition of a cocktail of protease inhibitors has often proved adequate. Facility staff will be pleased to advise collaborators on suitable strategies.

Gel Electrophoresis                                          
2D gel electrophoresis is the main technique used to separate proteins in the facility. It is suitable for analyzing the proteins present in complicated biological samples, but can be supplemented with a variety of biochemical techniques in order to concentrate proteins of interest. 2D electrophoresis is often suitable for separating highly complex protein mixtures, however some proteins will be masked by other more abundant species and many proteins will remain beyond the limit of detection. The pH range for the 1st dimension needs to be optimized, and this may consume considerable amounts of time and sample.

Image Analysis
Computer-aided comparison of 2D gels facilitates the identification of changes in protein mobility and abundance. The facility has two workstations equipped with PDQuest, a standard software package in proteomics. Gels can be stained with silver or Deep Purple using protocols that allow subsequent analysis by mass spectrometry. It is hoped to develop comparative fluorescence-based techniques at a later stage. Note that there is inherently some gel to gel variation in staining and mobililty; consequently changes are significant if they are reproducible and generally greater than 2 to 3-fold in intensity.

Excision and Digestion                                       
Spots from 2D gels that reproducibly differ between experimental and control samples are manually removed for digestion, usually with trypsin. As such it is not practical to excise large numbers of proteins from multiple gels. It is vital up to this stage that samples are not contaminated with environmental proteins (typically keratins). Proteolytic fragments can then be desalted and analysed directly on the mass spectrometer or separated using the CapLC HPLC system and automatically loaded onto the mass spectrometer.

Mass Spectrometry
The Micromass qToF-µ electrospray mass spectrometer is configured for high-sensitivity analysis of small numbers of samples. It is not a high-throughput instrumen and requires expertise to operate. It is not suitable for screening vast numbers of samples. Sensitivity is highly compromised by salts and detergents in the sample. In addition to peptide analysis, the qToF-µ is suitable for studying many intact proteins (contact the facility staff for details). Facility staff will advise you on the best buffer in which to prepare samples.

Bioinformatics                                                    
Data from the mass spectrometer consists of a series of molecular ion m/z (mass/charge) values that are translated into to a corresponding series of molecular weights or ”peptide fingerprint". Each fingerprint is then compared with the predicted fingerprints of all proteins in a comprehensive sequence database to identify the parent protein. In additionmolecular ions can be fragmented by collision in order to derive sest of daughter ions, from which information on amino acid sequence and sites of covalent modification can be obtained. Note that in general covalent modifications need to be stable and of high stoichiometry to be identified. The facility is equipped with a workstation running MassLynx 4.0 software. Facility staff will assist researchers with the identification of target proteins.

Useful references and web pages
British Mass Spectrometry Society
A basic mass spectrometry tutorial
Bio-Rad Proteomics Guide
International Mass Spectrometry News
American Society for Mass Spectrometry

Informace o projektu „Blue gene“ společnosti IBM-výzkum:

http://www.research.ibm.com/bluegene/


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