Figure 2. A lobster with a Tygon observation chamber enclosing carapace cuticle and a Teflon-nut glued to adjacent carapace. (A) The observation chamber encloses a region of cuticle with a lesion and holds a measured amount of minimal-ASW. This lobster is studied in (B) with dual SIET probes for proton and calcium flux. The large chamber in B holding the lobster is temperature regulated with Peltier cooled ASW that allows the lobster to be probed for hours and afterwards reserved to be probed on succeeding days. This way the progression of shell lesions can be followed over days or months. The objective is to identify candidate early lesions and extrapolate back to the origin of a lesion.
Figure 3. Birefringent Calcite of healthy lobster cuticle follows surface sculpturing. Top and bottom image are same view with analyzer rotated 90. Arrows show complementary birefringence of calcite layer on 90 rotation of analyzer.
Figure 4. Lobster calcite and carbonate-apatite structures interpreted as false color from three EMP of Ca (green), P (blue) and Cl (red) content.