An assessment of nucleic acid amplification testing for active mycobacterial infection



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Non-sputum

Sensitivity [95%CI]

Non-sputum

Specificity [95%CI]

Extrapulmonary

Sensitivity [95%CI]

Extrapulmonary

Specificity [95%CI]

AFB

All NAAT


In-house NAAT

Commercial NAAT

AFB (ZN)

AFB (FL)


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46% [37, 55] k=35

46% [34, 59] k=25

46% [35, 57] k=10

46% [34, 59] k=26

46% [35, 57] k=10



-

98% [97, 99] k=35

98% [96, 99] k=25

99% [98, 99] k=10

98% [96, 99] k=26

96% [90, 99] k=10



-

44% [33, 55] k=29

43% [29, 58] k=21

48% [34, 63] k=8

46% [31, 61] k=21

42% [32, 53] k=7



-

98% [96, 99] k=29

98% [96, 99] k=21

91% [81, 96] k=8

98% [95, 99] k=21

99% [97, 99] k=7



NAAT

All NAAT


In-house NAAT

Commercial NAAT



-

91% [83, 94] k=35

91% [79, 96] k=25

90% [83, 94] k=10



-

92% [84, 96] k=35

88% [75, 95] k=25

96% [90, 99] k=10



-

91% [83, 96] k=29

91% [78, 97] k=21

90% [83, 94] k=8



-

89% [79, 95] k=29

86% [70, 95] k=21

92% [85, 96] k=8



AFB + NAAT

All NAAT


In-house NAAT

Commercial NAAT



-

94% [91, 96] k=18

100% [87, 100] k=11

94% [90, 96] k=7



-

83% [70, 91] k=18

56% [11, 93] k=11

92% [82, 97] k=7



-

94% [91, 96] k=17

93% [87, 96] k=10

94% [91, 97] k=7



-

81% [68, 90] k=17

70% [49, 85] k=10

91% [81, 96] k=7



AFB = acid-fast bacilli; CI = confidence interval; FL = fluorescent staining; NAAT = nucleic acid amplification test; TB = tuberculosis; ZN = Ziehl-Neelsen staining

Forest plot showing the pooled sensitivity <a href=and specificity values for AFB, NAAT and AFB plus NAAT compared with culture, according to specimen type" align="bottom" width="937" height="424" border="0">

Figure 40 Forest plot showing the pooled sensitivity and specificity values for AFB, NAAT and AFB plus NAAT compared with culture, according to specimen type



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