Annual Report Form Technology Dissemination of Virus-free Seed Potato Production using Hydroponic Production systems in Thailand Country: Thailand Principal Investigator
Remark: Henfling, J.W. 1987. Late Blight of Potato (Phytophthora infestans)
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- (Positive reaction of potato)
- Bacteria; procedure to detect Ralstonia solanacearum by using GLIFT Kit
- 3. Results (optional) Discussion
- 5. Project Impacts Recommendations
- 6. Future Plan The Objectives
- Expected accomplishments
- Activities 201 7 201
Remark: Henfling, J.W. 1987. Late Blight of Potato (Phytophthora infestans). Technical Information Bulletin 4. International potato center (CIP). La Molina-Lima, Peru. 25 p. Virus; procedure to detect virus by using GLIFT Kit Complete grinding the small piece of tissue of potato (≈2x2 cm) in plastic bag with 1 ml of extraction buffer (Use 0.5-1 more ml of extraction buffer to dilute their sticky crude extraction) Put 3 drops of crude extraction of sample from No.1 in to well of GLIFT Kit check the reaction in 3-5 min. (Positive reaction of potato) 
Bacteria; procedure to detect Ralstonia solanacearum by using GLIFT Kit To confirm that seed potatoes are R. solanacearum-free, an efficient detection method is necessary. Several methods can be used for plant pathogenic bacteria diagnosis. The diagnosis from infested tissue by culturing on the media requires 5-7 days. Faster diagnosis methods such as Enzyme-linked Immunosorbent Assay (ELISA) or Dot Immunobinding Assay (DIBA) can be used to identify plant pathogenic bacteria because of their accuracy, quick and large number of samples can be tested at the same time, but these methods require approximately 5-6 hours. The ELISA test kit for R. solanacearum detection has been developed; however, this kit requires user’s skill and experience which limits the utilization in the fields. A more user friendly, the Gold labeled IgG flow test (GLIFT), kit was developed using serology technique in combination with Lateral flow test technique. It has high accuracy, precision and fast. The result could be obtained within 5-10 minutes regardless of user’s experience and skill. The GLIFT kit can be used for R. solanacearum testing in bacterial wilt-free certification program. The results obtained from the GLIFT kit were proved to be consistent with the laboratory results. Farmers can use the kit to test for seed potatoes prior to planting to reduce the disease in the field. The GLIFT kit can detect as low as 104 cfu/ml of R. solanacearum in seed potatoes sap, the violet color appears in the test line and the control line within 5 minutes. The bacteria detection Status of quality guarantee in the market The official inspectors are checked for pests, diseased, damage and defects before certified seed potato before distributed to famer/entrepreneur. 3. Results (optional) & Discussion - Discussion and Analysis of Findings - 4. Suggestions for project - Special conditions of each country 1) General suggestions - 2) (optional) Localization (modification) of hydroponics system, disease diagnosis and management, etc. - 5. Project Impacts & Recommendations The high temperature and unsuitable fertilizer ratio are affected to impact crops. Moreover, potato virus are seed-borne that it is reduce mass production of seed potato. Then, the technique of in vitro micropropagation in potato cv. Atlantic will be done. The technique of induce microtuber in solid and liquid media should be develop. The minituber production under aeroponic system should be improves technique, lower costs and increase the farmers’ income. Aeroponics is a soilless method for producing pre basic potato seed. The method can produce higher yields, easier, rapid, flexible and more security for the phytosanitary quality. 6. Future Plan The Objectives: To develop technologies for increase In vitro microtubers production of potato which disappear pathogens. To develop technologies for increase virus-free minituber potato production under aeroponic system. Expected accomplishments: Increased microtuber production in solid or liquid media. Increased yield production of virus-free G0 in aeroponic system.
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