Appendix 2-5: Rejected ecotox bibliography Excluded



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Journal of Environmental Science and Health Part B, Pesticides, Food Contaminants, and Agricultural Wastes Effects of combinations of malathion and cypermethrin on survivability and time of metamorphosis of tadpoles of Indian cricket frog (Fejervarya limnocharis).
Keywords: Frogs
Keywords: Pesticides
Keywords: Environmental Studies English. Copyright - Copyright 2012, Ecology, Environment & Conservation via NewsRx.com. Last updated - 2012-03-21. DOI - 2614358871; 68094362; 85211; EEVC; NWRX2012032117BF94D2

11. ++abar, Romina; Dolenc, Darko; Jerman, Tina; Franko, Mladen, and Treb+íe, Polonca. Photolytic and photocatalytic degradation of 6-chloronicotinic acid. 2011 Oct; 85, (5): 861-868.


Rec #: 990
Keywords: FATE
Notes: Chemical of Concern: MLN
Abstract: This work describes for the first time the photolytic and photocatalytic degradation of 6-chloronicotinic acid (6CNA) in double deionised water, which is a degradation product of neonicotinoid insecticides imidacloprid and acetamiprid, and it is known to appear in different environmental matrices. Photolytic experiments were performed with three UVA (ultraviolet A) polychromatic fluorescent lamps with broad maximum at 355 nm, while photocatalytic experiments were performed using immobilised titanium dioxide (TiO2) on six glass slides in the spinning basket inside a photocatalytic quartz cell under similar irradiation conditions. Photolytic degradation revealed no change in concentration of 6CNA within 120 min of irradiation, while the photocatalytic degradation within 120 min, obeyed first-order kinetics. The observed disappearance rate constant was k = 0.011 -_ 0.001 minęĆ1 and t1/2 was 63.1 -_ 5.5 min. Mineralisation rate was estimated through total organic carbon (TOC) and measurements revealed no carbon removal in case of photolysis after 120 min of exposure. However in photocatalytic experiments 46 -_ 7% mineralisation was achieved within 120 min of irradiation. Nevertheless, the removal of total nitrogen (TN) was not observed across all experiments. Ion chromatographic analyses indicated transformation of chlorine atoms to chloride and increase of nitrate(V) ions only via photocatalytic experiments. Efficiency of selected advanced oxidation process (AOP) was investigated through toxicity assessment with Vibrio fischeri luminescent bacteria and revealed higher adverse effects of treated samples on bacteria following photocatalytic degradation in spite of the fact that higher mineralisation was achieved. New hydroxylated product generated in photocatalytic experiments with TiO2, was confirmed with liquid chromatographyÇôelectro spray ionisation mass spectrometry (LCÇôESIÇôMS/MS) analyses, gas chromatographyÇômass spectrometry (GCÇôMS) and nuclear magnetic resonance spectroscopy (1H NMR). 6-Chloronicotinic acid/ Photolysis/ Photocatalysis/ TiO2/ Toxicity/ Product study http://www.sciencedirect.com/science/article/pii/S004565351100765X

12. ++abar, Romina; Komel, Tilen; Fabjan, Jure; Kralj, Mojca Bavcon, and Treb+íe, Polonca. Photocatalytic degradation with immobilised TiO2 of three selected neonicotinoid insecticides: Imidacloprid, thiamethoxam and clothianidin. 2012 Sep; 89, (3): 293-301.


Rec #: 1000
Keywords: FATE
Notes: Chemical of Concern: MLN
Abstract: This research focused on photocatalytic degradation of imidacloprid, thiamethoxam and clothianidin employing a tailor-made photoreactor with six polychromatic fluorescent UVA (broad maximum at 355 nm) lamps and immobilised titanium dioxide (TiO2) on glass slides. The disappearance was followed by high pressure liquid chromatography (HPLCÇôDAD) analyses, wherein the efficiency of mineralization was monitored by measurements of total organic carbon (TOC). Within 2 h of photocatalysis, all three neonicotinoids were degraded following first order kinetics with rate constants k = 0.035 -_ 0.001 minęĆ1 for imidacloprid, k = 0.019 -_ 0.001 minęĆ1 for thiamethoxam and k = 0.021 -_ 0.000 minęĆ1 for clothianidin. However, the rate of mineralization was low, i.e. 19.1 -_ 0.2% for imidacloprid, 14.4 -_ 2.9% for thiamethoxam and 14.1 -_ 0.4% for clothianidin. This indicates that several transformation products were formed instead. Some of them were observed within HPLCÇôDAD analyses and structures were proposed according to the liquid chromatographyÇôelectro spray ionization tandem mass spectrometry analyses (LCÇôESIÇôMS/MS). The formation of clothianidin, as thiamethoxam transformation product, was reported for the first time. Imidacloprid/ Thiamethoxam/ Clothianidin/ Photocatalysis with immobilised TiO2/ LCÇôMS/MS http://www.sciencedirect.com/science/article/pii/S0045653512005498

13. Abass, K.; Turpeinen, M., and Pelkonen, O. An evaluation of the cytochrome P450 inhibition potential of selected pesticides in human hepatic microsomes. 2009; 44, 553-563.


Rec #: 11640
Keywords: HUMAN HEALTH
Notes: Chemical of Concern: MLN
Abstract: Abstract: The goal of this work was to study the ability of 18 pesticides to inhibit selective model activities for all major xenobiotic-metabolizing enzymes, namely CYP1A1/2, 2A6, 2B6, 2C8, 2C9, 2C19, 2D6, 2E1 and 3A4. Generally organophosphorus insecticides were the most potent and extensive inhibitors, especially towards CYP1A1/2 (IC(50) values of chlorpyrifos, fenitrothion and profenofos similar to 3 mu M), CYP2B6 (IC(50) values of chlorpyrifos and fenitrothion 2.5 mu M), CYP2C8 (fenitrothion 4.3 mu M), CYP2C9 (fenitrothion and malathion 4.8 and 2.5 mu M, respectively), CYP2D6 (chlorpyrifos and phenthoate similar to 3 mu M) and CYP3A4 (chlorpyrifos, fenitrothion and phenthoate 3-4 mu M). Otherwise there were quite considerable differences in potency and extent of inhibition between different organophosphates. Pyrethroids were in general very weak or inactive. Deltamethrin and fenvalerate were potent inhibitors of CYP2D6 (IC(50) values of similar to 3 mu M) while lambda-cyhalothrin potently inhibited both CYP2D6 and CYP3A4-mediated activities (IC(50)'s about 3-4 mu M). Some pesticides caused relatively potent inhibitions sporadically (carbendazim, CYP2D6, IC(50) = 12 mu M; atrazine, CYP3A4, IC(50) = 2.8 mu M; glyphosate, CYP2C9, IC(50) = 3.7 mu M; hexaflumuron, IC(50) = 6.0 mu M). With the exceptions of alpha-cypermethrin, cypermethrin, isoproturon, carbaryl and abamectin, most pesticides inhibited relatively potently at least one CYP-selective activity, which may have relevance for potential interactions in occupational exposures and for further studies on the CYP-associated metabolism of respective pesticides.
Number of Volumes: 6
ISI Document Delivery No.: 535VX ://CCC:000273001100007

14. Abdel-Nasser, G; Al-Turki, a M; Al-Wabel, M I, and El-Saeid, M H. Behavior of Atrazine and Malathion Pesticides in Soil: Simulation of Transport Process Using Numerical and Analytical Models\. 2011 Mar; 5, (3): 221-235.


Rec #: 3610
Keywords: FATE
Notes: Chemical of Concern: MLN
Abstract: Abstract: The objectives of present study were to investigate the transport and distribution of Atrazine and Malathion in soil columns and to determine the transport parameters by using numerical and analytical models. The soil sample used in the study was collected from the surface layer of Kharj Province, Riyadh, Saudi Arabia. The present results showed that Atrazine was reached the lower boundary at only the high rate of water flux, but the low water flux did not drained from lower boundary. Both low and high water flux are able to move Malathion to the lower boundary with different magnitude. High and low water flux led to leach Atrazine downward and the maximum concentration was at 17.5 cm and 12.5 cm depth. In case of Malathion, the water flux leached Malathion out of soil column with high rate, while low rate led to concentrate the Malathion at lower depth of soil column. Numerical and analytical analysis did not differ from experimental data.
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15. Abdul Majeed, S; Nambi, Ksn; Taju, G; Sundar Raj, N; Madan, N; Sahul Hameed, as, and Abdul Majeed, S. Establishment and Characterization of Permanent Cell Line From Gill Tissue of Labeo Rohita (Hamilton) and Its Application in Gene Expression and Toxicology. 2013 Feb; 29, (1): 59-73.


Rec #: 2390
Keywords: IN VITRO
Notes: Chemical of Concern: MLN
Abstract: Abstract: Rohu gill cell line (LRG) was established from gill tissue of Indian major carp (Labeo rohita), a freshwater fish cultivated in India. The cell line was maintained in Leibovitz's L-15 supplemented with 10 % foetal bovine serum (FBS). This cell line has been sub-cultured more than 85 passages over a period of 2 years. The LRG cell line consists of both epithelial and fibroblastic-like cells. The cells were able to grow at a wide range of temperatures from 22 to 32 degree C, the optimum temperature being 28 degree C. The growth rate of gill cells increased as the FBS proportion increased from 2 to 20 % at 28 degree C. The plating efficiency was also high (34.37 %). The viability of the LRG cell line was 70-80 % after 6 months of storage in liquid nitrogen. The karyotype analysis revealed a diploid count of 50 chromosomes. The gill cells of rohu were successfully transfected with pEGFP-N1. Amplification of mitochondrial Cox1 gene using primers specific to L. rohita confirmed the origin of this cell line from L. rohita. The cytotoxicity of malathion was assessed in LRG cell line using multiple endpoints such as 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, Neutral Red assay, Alamar Blue assay and Coomassie Blue protein assay. Acute toxicity assay on fish was conducted by exposing L. rohita for 96 h to malathion under static conditions. Statistical analysis revealed good correlation with r super(2)=0.946-0.990 for all combinations between endpoints employed. Linear correlations between each in vitro effective concentration 50 and the in vivo lethal concentration 50 data were highly significant.
Keywords: Diploids
Keywords: Statistical analysis
Keywords: Mitochondria
Keywords: G 07730:Development & Cell Cycle
Keywords: Acute toxicity
Keywords: Freshwater fish
Keywords: bromides
Keywords: Freshwater
Keywords: Toxicity tests
Keywords: Karyotypes
Keywords: Malathion
Keywords: India
Keywords: Genetics Abstracts; ASFA 1: Biological Sciences & Living Resources; Toxicology Abstracts
Keywords: Gene expression
Keywords: Chromosomes
Keywords: X 24330:Agrochemicals
Keywords: Gills
Keywords: Toxicology
Keywords: Growth rate
Keywords: Temperature effects
Keywords: Data processing
Keywords: Freshwater environments
Keywords: Q1 01582:Fish culture
Keywords: Cyclooxygenase-1
Keywords: Cytotoxicity
Keywords: Labeo rohita
Keywords: Bioaccumulation
Keywords: Primers
Keywords: Nitrogen English. Date revised - 2013-02-01. Number of references - 62. Last updated - 2013-05-17. DOI - 16b636ef-a8ba-4b5d-bec8mfgefd107; 17668465; CS1308894; 0742-2091; 1573-6822. SubjectsTermNotLitGenreText - Temperature effects; Gene expression; Bioaccumulation; Diploids; Freshwater fish; Toxicity tests; Karyotypes; Toxicology; Gills; Growth rate; Data processing; Freshwater environments; Statistical analysis; Mitochondria; Acute toxicity; bromides; Malathion; Cyclooxygenase-1; Chromosomes; Cytotoxicity; Primers; Nitrogen; Labeo rohita; India; Freshwater. Ahmed, V. P. Ishaq; Chandra, V.; Sudhakaran, R.; Kumar, S. Rajesh; et al. Development and characterization of cell lines derived from rohu, Labeo rohita (Hamilton), and catla, Catla catla (Hamilton) JOURNAL OF FISH DISEASES, 32. 3 (2009): 211-218. WILEY-BLACKWELL PUBLISHING, INC. Anonymous. United States Environmental Protection Agency technical report. 2005. AVELLA, M.; BERHAUT, J.; PAYAN, P. Primary culture of gill epithelial cells from the sea bass Dicentrarchus labrax. In vitro cellular & developmental biology, 30A. 1 (1994): 41-49. Society for In Vitro Biology. Babich, H; BORENFREUND, E. CYTOTOXICITY AND GENOTOXICITY ASSAYS WITH CULTURED FISH CELLS - A REVIEW. TOXICOLOGY IN VITRO, 5. 1 (1991): 91-100. PERGAMON-ELSEVIER SCIENCE LTD. Babich, H; Rosenburg, D W; Borenfreund, E; Babich, H. In vitro cytotoxicity studies with the fish hepatoma cell line, PLHC-1 (Poeciliopsis lucida). Ecotoxicology and Environmental Safety, 21. 3 (1991): 327-336. Babu, V. Sarath; Chandra, V.; Nambi, K. S. N.; Majeed, S. A.; et al. Development and characterization of novel cell lines from Etroplus suratensis and their applications in virology, toxicology and gene expression. JOURNAL OF FISH BIOLOGY, 80. 2 (2012): 312-334. WILEY-BLACKWELL. Bejar, J; Borrego, J J; Alvarez, M C; Bejar, J. A continuous cell line from the cultured marine fish gilt-head seabream (Sparus aurata L.) 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16. Abou Zeid, A. A.; Hussein, H. A., and Shahin, A. A. Aspects of High Concentrations of Certain Organophosphorus Insecticides on the Activity and Structure of Some Biodegrading Soil Fungi and Their Response to gamma Radiation. 2002; 1, 40-60.


Rec #: 420
Keywords: NO SOURCE
Call Number: NO SOURCE (MLN), OK (PFF)
Notes: Chemical of Concern: MLN,PFF

17. Adamczyk, Sabine; Lazaro, Regina; Perez-Arquillue, Consuelo; Bayarri, Susana; Herrera, Antonio, and Lazaro, Regina. Impact of the Use of Fluvalinate on Different Types of Beeswax From Spanish Hives. 2010 Apr; 58, (3): 733-739.


Rec #: 7050
Keywords: SURVEY
Notes: Chemical of Concern: MLN
Abstract: Abstract: Acaricides are applied in agriculture as phytosanitary products against pests and in apiculture to control the bee parasite Varroa destructor. Poor apicultural practices could result in an accumulation of residues in honeybees, in the environment, and in beeswax and other bee products by migration from the wax comb into stored honey through a process of diffusion and consequently constitute a potential risk for humans. In this study, six different types of


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