Rosehip Extracts Prevent Glioblastoma Cell Proliferation by Regulating Retinoblastoma Phosphorylation
Glioblastoma multiforme (GBM) are malignant tumors that arise in the brain. The current system of clinical management for GBMs consists of surgical resection followed by radiotherapy and chemotherapy. However, this treatment regimen has not improved the lifespan of GBM patients in the past twenty years and new therapeutic options are being aggressively investigated. Rosehip (Rosa canina) extracts have been used for centuries as alternative therapies. Recent studies demonstrate that rosehip extracts possess anti-tumor properties. Therefore, we tested the antiproliferative capacity of rosehip extracts against GBM cell lines. We hypothesize that human GBM cell lines exposed to rosehip extract will demonstrate lower rates of cell proliferation as a result of cell cycle regulation. The human GBM cell lines, U-251 MG and U-1242 MG were treated with rosehip extracts (1 mg/mL - 25ng/mL) and demonstrated a decrease in cell proliferation. Utilizing a fluorescent-based labeling strategy (Live-Dead Assay), we examined whether rosehip extracts prevented cell proliferation by initiating apoptosis. Pretreatment with rosehip extracts (1 mg/mL - 25ng/mL) induced the inhibition of cell proliferation without promoting apoptosis. Furthermore, we assayed the phosphorylation level of the retinoblastoma (Rb) protein to determine the mechanisms by which rosehip extracts were preventing cell proliferation. Rb phosphorylation was decreased following exposure to the rosehip extracts, suggesting the extracts prevent cell cycle progression beyond the G1 phase. Taken together, these data suggest that rosehip extracts inhibit cell proliferation via a cytostatic mechanism that prevents cell cycle progression
1 Biology, North Carolina Agricultural and Technical State University, Greensboro, NC; 2 Animal Sciences, North Carolina Agricultural and Technical State University, Greensboro, NC; 3 Family & Consumer Sciences, North Carolina Agricultural and Technical State University, Greensboro, NC
P63 • Jamal Shuja1, Shirley A. Vas1, Vanessa Yanes1, Michael Erwin
Haplotype Diversity Analysis of Black Crappie, Pomoxis nigromaculatus (Family Centrarchidae), Inhabiting Twofloridian Lakes
Molecular markers have been used to analyze the genetic structure of many pelagic fish populations. In this study haplotype diversity will be examined for the nuclear rhodopsin gene (RHO) and the mitochondrial cytochrome c oxidase I gene (COI) of Pomoxis nigromaculatus, Black Crappie, in Lake Sydney Lanier, Georgia. Data released by the U.S. Dept of the Interior indicates that P. nigromaculatus is the highly targeted freshwater fish in United States. This fishery has been sustainable due to the species’ fecundity, monitoring by wildlife management, and enforced bag limits by the state. Data on the genetic structure of this population may provide a useful tool for wildlife managers to more effectively manage this species. Mitochondrial DNA (mtDNA) and nuclear DNA (nuDNA) sequence data were collected for the purpose of developing an initial genetic baseline for black crappie inhabiting Lake Marion and East Lake Tahoe, Florida.
1 School of Science and Technology Georgia Gwinnett College
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P64 • Bryan T. Zorn1, Cara M. Santelli2, Sarah K. Carmichael3, Chuck P. Pepe-Ranney4, Leah-Anne Roble5, Mary-Jane Carmichael6, Suzanna L. Bräuer1
Illumina Sequencing of Fungi Associated With Manganese Oxide Deposits in Karst Systems
Fungi have been observed to produce external Mn(III/IV) oxides resembling buserite, birnessite, and todorokite. Although the exact evolutionary function and genetic trigger producing these oxides is unclear, they can influence the environment by aiding in the breakdown of recalcitrant carbon as well as offering a versatile protective barrier immobilizing other metals. In this study, native and manipulated microbial communities associated with manganese oxide deposits within two karst systems are compared and contrasted by using molecular techniques. While qPCR data of sampled sites indicate that fungi are present in relatively low abundance, diversity remains high. Using ultra-deep sequencing in conjunction with the QIIME pipeline, millions of paired-end fungal ITS1 amplicons have yielded a plethora of OTUs providing unprecedented insight into phylogenetic assemblages of this unique biome, as well as identifying many genera of known manganese oxidizers that may be contributing to said deposits such as Acremonium, Cladosporum, Mortierella, Pleospora, and Pyrenchaeta.
1 Biology Dept, Appalachian State University, Boone, NC; 2 Mineral Sciences, Smithsonian Institution, Washington, DC; 3 Geology Dept, Appalachian State University, Boone, NC; 4 Crop and Soil Sciences, Cornell University, Ithaca, NY; 5 Geology Dept, University of Maryland, College Park, MD; 6 Biology Dept, Wake Forest University, Winston-Salem, NC
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P65 • C. Ronnie Funk1, Melanie May2, Anna Blenda1, Charles E. Schwartz2
Investigation of the Molecular Basis of Split Hand-Foot Malformation in a Family by Whole Exome Sequencing (WES)
Split hand foot malformation (SHFM), ectrodactyly, is a limb malformation disorder that involves the central rays of the hands and feet. People with SHFM can exhibit syndactyly, median clefts of the hands and feet, and aplasia or hypoplasia of the phalanges, metacarpals, and metatarsals. While several chromosomal loci have already been associated with SHFM, the molecular basis of SHFM remains unknown in some families. In order to determine the etiology of SHFM in a family without mutations in known SHFM genes, genomic DNA of some family members was subjected to whole exome sequencing (WES), The data was filtered to identify gene alterations that were novel, not present in normal databases. Based on this analysis, missense mutations in three genes, MAP1B, NEK1, and CHD6 were selected for further analysis. Segregation analysis of the mutations in the family initially indicated that only the change in MAP1B might be relevant to the phenotype. Unfortunately, the follow up analysis of twins, one of which was clearly affected, born to an affected male, failed to provide segregation of the MAP1B mutation. As a result, none of the three gene alterations detected by WES were found to be associated with the SHFM phenotype in the family. The data is being re-analyzed to determine if other potential mutations exist which can be pursued in the family.
1 Dept of Biology, Erskine College, Due West, SC; 2 Center for Molecular Studies, Greenwood Genetic Center, Greenwood, SC
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P66 • Sarah Brown, Marlee B. Marsh
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