CHAPTER THREE MATERIALS AND METHODS 3.0 Materials and Methods - - - - - - - - - 36
3.1 Study Area - - - - - - - - - - -36
3.2 Sample Collection - - - - - - - - - 38
3.2.1 Sample Procedure - - - - - - - - - 38
3.2.2 Histological Examination - - - - - - - - 38
3.2.3 Measurment of Oocyst/Sporocyst and Sarcocysts - - - - - 39
3.3 Statistical Analysis - - - - - - - - - 39
CHAPTER FOUR RESULTS 4.0: Result - - - - - - - - - - - - 40
CHAPTER FIVE DICUSSION, CONCLUTION AND RECOMMENDATIONS 5.0: Discussion - - - - - - - - - - 48
5.1: Conclusion - - - - - - - - - - 50
5.2: Recommendation - - - - - - - - - 51
References - - - - - - - - - - - 52
Appendix - - - - - - - - - - - 54
LIST OF TABLES Tables Pages 2.1: Prevalence of Sarcocystis macrocysts in the muscles of different - - 28 Organs in 49 sheep with macrocyst from 4 different age group
2.2: The prevalence of Sarcocystis macrocysts determined by the Histological - -29 examination in the muscles of different organs In 200 sheep from 4 different age groups
4.1: Detection rate of Sarcocystis species in cattle - - - - - - 42
4.2: Detection rate of Sarcocystis species in goats - - - - - - 43
4.3: Detection rate of Sarcocystis species in pigs - - - - - - 44
LIST OF PLATES Plates Page Number Plate 1: Sarcocystis spp. Histological section with H & E. 1) --------------------------18
Plate 2: Sarcocystis of S. miescheriana in skeletal muscles of pigs -------------------22
Plate 3: Longitudinal section in oesophagus muscles -----------------------------------24
of goats infected with Sarcocystis
Plate 4: Photomicrograph of bradyzoites released from --------------------------------33
sarcocyst in the esophagus of pig during tissue
digestion stained with Giemsa
Plate 5: Photomicrograph of elongated cyst seen in the oesophagus of cattle ------45
Plate 6: Photomicrograph of elongated cyst seen in the esophagus of pig -----------46
Plate 7: Photomicrogragh of multiple oval cyst seen in the oesophagus of goat ---47
