Rna expression patterns change dramatically in human neutrophils exposed to bacteria
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 ,
where  is the two-tailed t-value of a t-distribution with n-2 degrees of freedom.
The accuracy of such confidence limits depends on the validity of the assumption of linearity and equally normal distributions of Y values across all values of X. However, measurements by the PhosphorImager System at very low intensities are much less reliable. Therefore we fit the data to a linear regression model based on measurements at X>1, yielding  and a correlation of  . For all  .
Quantitative measurement of Northern blots of several mRNAs confirm that genes identified by gel display to be up- or down-regulated do indeed show increases or decreases mRNA levels. These changes range from a ten-fold decrease to a 71-fold increase (Table 1), and the logarithm of the values correlated to estimates from the gel display method (Pearson correlation  ).
Each sequence was searched against nr and dbest databases of NCBI by the BLAST program.29 Matches to known genes were confirmed to come from the 3’-untranslated regions of mRNAs except where otherwise noted. The length of sequence obtained was compared with the size of bands on the display gel as a quality check. A database was created by Shigeru Yamaga, jointly with Wen Ming Xiao of Gene Logic Inc. (Gaithersburg, MD), using Microsoft Access® as a database engine. An individual record was created for every differentially expressed band, and related information was entered as hypertext links to sequence files, search results of GenBank and TIGR databases, bands of overlapping sequence, references to relevant literature, keys for various classifications of bands, presence of polyA signals, quality of sequence, scanned gel images etc. We used LocusLink ID (http://www.ncbi.nlm.nih.gov/LocusLink/) as a unique key to known genes, if available, and used the terms listed as “Gene Symbol” (the HGMW-approved symbol, where applicable) and “Gene Names”. For ESTs, we used UniGene cluster numbers (http://www.ncbi.nlm.nih.gov/UniGene/) as a unique key. Subsequently all sequences were clustered by a modified PHRAP approach.30 Public gene database search was completed on Nov. 9, 2000. Directory: expression expression -> Contents: expression 1 expression -> Biochemistry Lab Expression and Purification Homework Assignment expression -> Hse research Awards on Ageing 2017 Expression of Interest Form, May 2017 expression -> Annual report of the office of the special rapporteur for freedom of expression expression -> Inter-american commission on human rights expression -> Internet shutdowns by Governments in West and Central Africa, and non-compliance with international law require urgent action Download 0.71 Mb. Share with your friends:
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