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APPENDIX I
Table 2. Normalized Ct Data for TaqMan qPCR at 7 Days after Optic Nerve Crush
Detector
|
Sample
|
Normalized Ct Sample
|
Avg Normalized Ct
|
Avg Normalized St Dev
|
SOCS2 Exon 2
|
Unoperated Eye
|
19.30
|
18.23
|
1.12
|
17.08
|
18.32
|
Operated Eye
|
15.83
|
15.91
|
0.13
|
16.05
|
15.85
|
Brain
|
17.76
|
18.12
|
0.64
|
17.74
|
18.86
|
No Template
|
N/A
|
|
|
SOCS2 No Exon 2
|
Unoperated Eye
|
19.76
|
19.07
|
0.72
|
18.32
|
19.12
|
Operated Eye
|
21.10
|
20.46
|
1.04
|
19.26
|
21.01
|
Brain
|
16.48
|
16.92
|
0.39
|
17.24
|
17.04
|
No Template
|
N/A
|
|
|
Table 3. Normalized Ct Data for TaqMan qPCR at 3 Days after Optic Nerve Crush
Detector
|
Sample
|
Normalized Ct Sample
|
Avg Normalized Ct
|
Avg Normalized St Dev
|
SOCS2 Exon 2
|
Unoperated Eye
|
16.41
|
16.09
|
0.30
|
15.81
|
16.05
|
Operated Eye
|
18.48
|
18.42
|
0.28
|
18.67
|
18.11
|
Brain
|
18.98
|
21.07
|
3.53
|
19.07
|
25.14
|
No Template
|
44.13
|
|
|
SOCS2 No Exon 2
|
Unoperated Eye
|
15.04
|
15.05
|
0.05
|
15.10
|
15.01
|
Operated Eye
|
12.99
|
13.19
|
0.28
|
13.51
|
13.08
|
Brain
|
15.28
|
14.89
|
0.37
|
14.86
|
14.53
|
No Template
|
N/A
|
|
|
APPENDIX II
No IRES Insert
pIRES2-DsRed2 IRES
SOCS2 5’UTR without Exon 2
SOCS2 5’UTR with Exon 2
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