Pineapple and Gelatin Enzyme Lab

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Background Information: Ask an expert.

  • Gelatin makes Jello a semi-solid. 1845
  • Gelatin comes from bones and cartilage from pigs and cows
  • Enzyme Bromelain is in Pineapple
  • Vitamin C is a co-enzyme to Bromelain
  • Bromelain helps relieve pain in arthritis.
  • Bromelain breaks down mucus and helps those with arthritis and bronchus

Investigative Question:

  • How do different types of pineapple affect gelatin?
  • Hypothesis:
  • If pineapple is put into gelatin, then ________

  • Enzymes are
  • Protein catalyst (starter) for chemical reactions cut (Catabolism) or add (Anabolism) substances in cells.

Anabolism: Catabolism in Jello

  • Anabolism: Starch (Carbohydrates) in the gelatin get thicker as it cools.
  • 0 + 0 + 0 = 000
  • Catabolism: Bromelain (protein) in pineapple break the solution apart: (prevent it from getting thick.
  • 000 + Protein Enzyme = 0 + 0 + 0 +


  • Fresh pineapple 2 pieces
  • Canned Pineapple 2 pieces
  • Gelatin (Jell-O ingredient)
  • May redo with Apple, Orange or other thing you bring in. It must be authorized by the teacher
  • 4 Dixie cups
  • Ice Tub
  • Ice
  • Stir sticks


  • 2. Put 2 pieces (about the same amount) of each type of pineapple different Cups.
  • 3. Take the Dixie cup to the gelatin and pour enough to cover the pineapple
  • 4. Only pour gelatin into Dixie Cup # 4.


  • Control Group: No pineapple, just gelation
    • (Check to see if there is something else making changes in the gelation)
    • Manipulative Variables: fresh and frozen pineapple, other
    • Responding Variable: What happens to the gelation
    • Experimental Controls: Same pineapple, same temperature, same gelation, same ___ same____

5. Put the cups in a pan of ice with the ice to the

  • 5. Put the cups in a pan of ice with the ice to the
  • Top level of the gelatin/pineapple.
  • 6. Let the Dixie cups sit in ice for up to 10 minutes checking the control every 2 minutes by swirling or stirring the Dixie cups.
  • 7. Record observations on a data table.
  • 8. When finished, pour semi-solid mixtures into waste beaker - NOT DOWN THE DRAIN!
  • 9. Clean lab thoroughly.
  • 10. Observe other groups work. Record findings


  • Data Table
  • 4 min
  • 10 min
  • Fresh
  • Canned
  • Control
  • Experimental (one tested to) Variable 1:
  • Responding (results) Variable:
  • Experimental Controls: (same/same)
  • Analysis Questions:
  • Describe the differences between Cups.
  • What was it like when you started and at the end?
  • Does the pineapple enzyme Bromelain breakdown (catabolism) or build (anabolism) proteins?
  • After looking at the data, what is the best explanation for the results of experiment?
  • How could you increase validity.
  • How could you increase reliability?


  • Answer to the investigative question.
  • Data to support the answer to the investigative question.
  • Address any trends you see in the data. (What happened?)
  • Explain why the trend in data occurred.

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