13th balkan biochemical biophysical days & meeting on metabolic disorders’ programme & abstracts
Gergana STOILKOVA, Petranka YONOVA
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- HYDROPHOBIC NATURE of RAT LYMPH CHYLOMICRONS
Gergana STOILKOVA, Petranka YONOVAAcad. M. Popov Institute of Plant Physiology, Bulgarian Academy of Sciences, Acad. G. Bonchev St, Bl.21, 1113 Sofia, Bulgaria geristo@mail.bulgaria.com The efficacy of two new synthetic compounds – 1-[4-fluorophenyl(thio)carbamoyl]-4-methyl-piperazines, B-6 and B-3 – as protectants of corn (Zea mays, L.) against injury fom preemergence application of the herbicide chlorsulfuron was determined. The corn seeds were impregnated with B-6 and B-3 by soaking the seeds for 5h in their solutions (0.5 and 1.0mM, respectively). Chlorsulfuron was applied at 10.0M for 5h immediately after compounds treatment. At least three independent parallel experiments were carried out in each case. The significant differences between mean values were evaluated by Student’s t-test. Differences were considered to be significant at p<0.05. Corn shot growth in length and fresh weight was partialy protected by B-6 from the phytotoxic effect of the herbicide. Protein and free amino acids contents were significantly increased in corn leaves treated with B-3 or B-6 and herbicide. These changes were accompanied by decreases in the activities of GPOA and CAT in samples treated with B-3 and herbicide, while a reverse relation was found in samples treated with B-6 and chlorsulfuron. Decreases in AsPOA activity was observed for the both compunds. The results have demonstrated the protective effect of both compounds against chlorsulfuron, furthermore B-6 (with thiocarbamoyl group) was more active than B-3. P267 HPYERLIPIDEMIA TREATMENT WITH ATORVASTATIN : HOMOCYSTEINE AND NITRIC OXIDE Çiğdem YENİSEY1, Nihat ÖZGEL2, Mukadder SERTER1, Zahit BOLAMAN2
OBJECTIVE: To determine the efficacy of atorvastatin to mild hyperlipidemia, hypercholesterolemi, and hyperhomocysteinemia. and to test the hypothesis that endothelial NO elaboration is impaired in hypercholesterolemi, and hyperhomocysteinemi. METHODS: Patients (n=44) were randomly assigned to 6 months of treatment with atorvastatin (10 mg/day) and also control individuals (n=30) were selected according to their serum parameters except homocysteine measurements. Homocysteine (HCY), B12 and folate levels were determined via chemiluminescent enzyme immunometric method via IMMULATE I hormone analyzer. RESULTS: Part of our results as follows: Parameters Before reatment After treatment Control HYC(mol/L) 14.47 8.75 16.03 8.95 10.99 2.26a B12 (pg/ml) 350 120.14 396.26 107.28c 400.67 78.63b Folate (ng/ml) 9.45 3.25 9.82 3.73d 11.21 2.16d T. Kol (mg/dl) 301 34.59 199.9 28.23f 171.53 20.03e TG (mg/dl) 198.77 87.79 138.72 53.94h 94.66 21.25g NO (nitrite+nitrate)
(a=0.015, b=0.034, d=0.007, e=0.000, g=0.000, ı=0.000) as compared with before treatment (c=0.021, f=0.000, h=0.000) as compared with before treatment CONCLUSIONS: Treatment with atorvastatin for 6 months was effective in reduction of T. Kol and TG levels, but not in mild hyperhomocysteinemia. Also, atorvastatin was improved endothelial dependent vasodilatation which indicating that bio-availability of NO is decreased in those with hypercholesterolemi. P268 HYDROPHOBIC NATURE of RAT LYMPH CHYLOMICRONSTayfun GÜLDÜR1,Aysun Bay KARABULUT1,Nihayet BAYRAKTAR1, Özgür KAYNAR2 1 Department of Biochemistry, Faculty of Medicine,İnönü University (Malatya/ TURKEY)2 Department of Biochemistry ,Institute of Health Sciences, Fırat University (Elazığ/TURKEY) Some of the hydrophobic characteristics of rat lymph chylomicrons were investigated. Thoracic duct was cannulated and the lymph was collected overnight. Chylomicrons (>100nm) were isolated by ultracentrifugation at 4x106g.min. Since the particle aggregation is a characteristic of hydrophobic nature of lipoproteins, as an index of aggregation, the turbidity generated by vortexing and storage of chylomicrons was measured spectrophotometrically at 680nm. In contrast to LDL, neither shaking nor prolonged storage at 4oC produced an increase in the optical density of chylomicron solution indicating no aggregation took place. In a second series of experiment, ability of chylomicrons to interact with five different hydrophobic interaction chromatography media (phenyl sepharose high performance, phenyl sepharose 6 fast flow (low substance), phenyl sepharose 6 fast flow (high substance), butyl sepharose 4 fast flow and octyl sepharose 4 flow. Typical elution profiles of chylomicrons through octyl, phenyl (high substance) and butyl sepharose columns showed two peaks. Peak I material emerged with 4M NaCl in a position corresponding to the void volume and peak II material eluted with water. Phenyl sepharose (high performance) media exhibited the maximum binding strength towards chylomicrons among the five different media.In the case of phenyl sepharose (low substance) column, an additional material was eluted with 3 M NaCl between peak I and II. These results indicate the heterogeneity of chylomicron surface hydrophobicity. Since the particle aggregation is a characteristics of hydrophobicity of lipoproteins and the aggregation is believed to be the underlying cause of atherosclerosis, fractionation of lipoproteins by hydrophobic interaction chromatography may introduce a new approach into the assessment of lipoprotein atherogeneicity. Key Words: Aggregation, chylomicron, hydrophobicity, atherosclerosis, chromatography
M. Tudorie, D.E. Creanga, C. Murgulet* Univ. Al. I. Cuza, Fac. of Physics, Biophysics and Medical Physics, 11 A, Bd. Carol I,, Iasi-Romania, e-mail:dorina.creanga@email.ro *University Hospital, Iasi, Romania Fractal analysis was carried out using adequate soft packages for the calculation of fractal dimension for two types of medical images: tomography films and electro graphical recordings (variant of Kirlian images obtained in the radiology clinic of the University Hospital and respectively in the Medical Physics Laboratory). The mathematical method utilized for the fractal dimension calculation was based on the ‘box-counting’ algorithm. The main type of tomographical image was the result of brain investigation on the basis of a Siemens Computer Tomograph; it was revealed that brain tumors leads to the increase of fractal dimension with about 10%. The electro graphical images were obtained using an electrostatic device designed and assembled by us, hands and feet of normal subjects in comparison to pathological being studied by means of the fractal dimension. Statistical analysis of the differences between the values provided by groups of normal and pathological cases showed significant results for brain tomography images as well as for electro graphical images of the hands but non-significant differences were revealed for feet images. References 1] B. Mandelbrot, The Fractal Geometry of Nature, Academic Press, 1975, New York [2] G. Hartvigsen, The analysis of leaf Shape using Fractal geometry, The American Biology Teacher, 62, (9), 2000, 663-665
A RAT DEMENTIA MODEL BY CHRONIC ETHANOL CONSUMPTION AND WITHDRAWAL: VALIDATION BY PASSIVE AVOIDANCE MEASUREMENT AND SERUM CHOLINESTERASE LEVEL Erdinç ÇAKIR1, Turgay. ÇELİK2, Hakan KAYIR2, Cumhur BİLGİ1, and I. Tayfun UZBAY2
The aim of the present study was to investigate if the chronic ethanol administration by liquid diet to rats may be a dementia model. Female Wistar rats (188-244 g) were used in the study. Ethanol was administered by a modified liquid diet with 4.8% (v/v) ethanol for 3 days followed by 25 days on a liquid diet in which the ethanol concentration was increased to 7.2%. Control rats were pair fed with an isocaloric liquid diet not containing ethanol. Serum ChE activity and blood ethanol concentration were measured at the end of the 4.8% ethanol consumption and after 35 days of ethanol (7.2%) feeding and, just before, 24th and 72nd hours ethanol witdrawal period. Cognitive functions were evaluated by step-down passive avoidance test system for 150 sec (cut-off time) in three individual groups of ethanol-administered, ethanol withdrawn (24th h withdrawal) and control rats. The data was evaluated by one-way analysis of variance followed by Tukey’s test for post-hoc comparison. The daily ethanol consumption of the rats ranged from 11.5 to 14.9 g/kg. ChE activity was found significantly increased from 3rd day of ethanol (4.8%) consumption. Serum ChE activities of the rats receiving ethanol (7.2%) also increased significantly as compared to ethanol (4.8%) ingesting rats. Blood ethanol levels were measured as 200 and 2.2 mg/dl at 35th days of ethanol consumption (just before ethanol withdrawal) and 24th h of ethanol withdrawal, respectively. Passive avoidance latency was found significantly reduced in the groups that just before and 24th h of ethanol withdrawal as compared to control rats. Our results suggest that serum ChE activity increased by chronic ethanol consumption in rats and chronic ethanol caused some marked impairments on the cognitive functions Overall the data indicated that chronic ethanol feeding might be a model for evaluation cognitive functions in rats.
Tamer ZEREN1, Mustafa ÖZBEK2, Nuran EKERBİÇER2, K.Gediz AKDENİZ3 Directory: 2003 2003 -> Tr-41. 4-03-05-024 Telecommunications 2003 -> Review of some tsunamis in canada 2003 -> The skill of multi-model seasonal forecasts of the wintertime North Atlantic Oscillation 2003 -> 2930 Kerry Forest Parkway 2003 -> Canadian Interuniversity Sport (cis) Athletes of the Week 2003 -> Senior Airman Jason Plite Hometown 2003 -> Technical announcements: My apologies for the comparison pages; they were 2003 -> Pool Play Round 1 9: 20: 00 am 2003 -> 2004 Division II men's Golf Championships Final Round Results deland, fla 2003 -> Go Figure Edited by Kostya Kennedy and Mark Bechtel 645 Passing yards, an ncaa division II record, by University of Indianapolis quarterback Matt Kohn, in a 59-52 victory over Michigan Tech Download 1.91 Mb. Share with your friends:
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