Davis et al. (2009)
Uganda
|
Level II
Low risk of bias
|
L-J Middlebrook and MGIT cultures
|
N=100 sputum samples
-
n=63
n=37
|
AFB (ZN)
secA1 PCR
AFB + NAAT
AFB +ve: secA1 PCR
AFB –ve secA1 PCR
|
55
62
63
54
8
|
8
6
11
3
3
|
8
1
0
1
0
|
29
31
26
5
26
|
27/127 AFB microscopy results not recorded and excluded from analysis
|
Mashta et al. (2011)
India
|
Level III-1
Low risk of bias
|
L-J liquid medium culture
|
N=463 sputum samples
-
n=228
n=235
|
AFB (MB)
IS6110 -devR PCR
AFB + NAAT
AFB +ve IS6110 -devR PCR
AFB –ve IS6110 -devR PCR
|
148
69
157
60
9
|
80
71
115
32
35
|
44
123
39
88
39
|
191
200
152
48
152
|
Contaminated samples (4%) were identified and patients were called to collect a second sample
|
Nakiyingi et al. (2012)
Uganda
|
Level III-1
Some risk of bias
|
L-J liquid medium culture
|
N=181 patients with AFB –ve sputum samples
|
AFB –ve IS6110 PCR
|
48
|
75
|
16
|
42
|
5/205 cultures were contaminated
19/205 PCR results were unavailable
|
Ekrami et al. (2011)
Iran
|
Level III-1 Some risk of bias
|
L-J solid medium culture
|
N=152 sputum samples
|
AFB (ZN)
IS6110 PCR
IS6110 nPCR)
|
90
78
94
|
6
8
6
|
16
28
12
|
40
38
40
|
None reported
|
Chakravorty et al. (2006)
India
|
Level III-2
Low risk of bias
|
L-J solid medium culture
|
N=506 sputum samples from 506 patients
|
USP AFB (ZN)
devR PCR
IS6110 PCR
|
273
269
272
|
39
52
65
|
0
4
1
|
194
181
168
|
None reported
|
Michelon et al. (2011)
Brazil
|
Level III-2
Low risk of bias
|
Culture
|
N=469 sputum specimens
N=295 induced sputum specimens
N=174 spontaneous sputum specimens
|
AFB
IS6110 PCR
AFB + NAAT
AFB
IS6110 PCR
AFB + NAAT
AFB
IS6110 PCR
AFB + NAAT
|
62
65
75
55
51
60
7
14
15
|
7
7
12
5
5
10
2
2
2
|
14
10
1
6
9
1
8
1
0
|
386
387
381
229
230
224
157
157
157
|
4/476 (3 induced and 1 spontaneous) specimens were PCR inhibitory
3/479 (induced) gave indeterminate results
|
Jiang et al. (2012)
China
|
Level III-2
Low risk of bias
|
MGIT and/or L-J culture
|
N=207 sputum samples
-
n=51
n=156
|
AFB (ZN)
16S qRT-PCR
AFB + NAAT
AFB +ve: 16S qRT-PCR
AFB –ve: 16S qRT-PCR
|
24
28
28
24
4
|
27
21
43
5
16
|
6
2
2
0
2
|
150
156
134
22
134
|
None reported
|
Suzuki et al. (2006)
Japan
|
Level III-2
Low risk of bias
|
MGIT culture
|
N=138 sputum specimens
n=55
n=83
|
: AFB
dnaJ PCR-ICA
AFB + NAAT
AFB +ve: dnaJ PCR-ICA
AFB –ve: dnaJ PCR-ICA
|
53
41
57
39
1
|
2
5
5
2
3
|
20
32
16
14
19
|
63
60
60
0
60
|
None reported
|
El Khechine et al. (2009)
France
|
Level III-2
Low risk of bias
|
BACTEC 9000 liquid culture
|
N=134 sputum samples
N=134 stool samples
|
AFB (ZN)
IS6110 qPCR
AFB + NAAT
|
13
18
18
|
2
10
10
|
7
0
0
|
112
106
106
|
None reported
|
George, Mony & Kenneth (2011)
India
|
Level III-2
Low risk of bias
|
MGIT and/or L-J medium culture
|
N=71 sputum samples
-
n=33
n=38
|
AFB (FL)
LAMP
AFB + NAAT
AFB +ve: LAMP
AFB –ve: LAMP
|
32
31
32
29
2
|
1
2
3
2
0
|
7
8
7
1
7
|
31
30
29
1
29
|
7/78 samples showed contamination for both L-J and MGIT cultures and were omitted from analysis
|
Deshmukh et al. 2013
India
|
Level III-2
Low risk of bias
|
MGIT and/or L-J culture
|
N=27 sputum specimens
|
AFB (ZN)
IS6110 PCR
AFB + NAAT
|
5
13
13
|
1
3
3
|
9
1
1
|
12
10
10
|
None reported
|
Ani et al. (2009)
Nigeria
|
Level III-2
Low risk of bias
|
L-J slope culture
|
N=10 sputum specimens from children
|
AFB –ve IS6110 PCR
|
4
|
0
|
6
|
0
|
None reported
|
Chakravorty et al. (2005)
India
|
Level III-2
Some risk of bias
|
L-J solid medium culture
|
N=571 samples from 571 patients
|
direct AFB (ZN)
USP AFB (ZN)
IS6110 PCR
|
322
225
325
|
21
18
70
|
6
103
3
|
222
225
173
|
None reported
|
Santos et al. (2006)
Brazil
|
Level III-2
Some risk of bias
|
L-J medium culture
|
N=214 sputum samples
-
n=46
n=168
|
AFB (direct)
AFB (conc.)
IS6110 PCR
AFB (conc.) + NAAT
AFB +ve: IS6110 PCR
AFB –ve: IS6110 PCR
|
14
17
38
38
17
21
|
1
4
24
26
2
22
|
30
27
6
6
5
1
|
169
166
146
144
22
124
|
4/218 samples were excluded due to contamination of culture tubes
|
Lee, Chen & Peng (2009)
Taiwan
|
Level III-2
Some risk of bias
|
Culture (not specified)
|
N=150 sputum samples
|
AFB
LAMP
|
30
32
|
9
7
|
4
2
|
107
109
|
None reported
|
de Albuquerque et al. (2014)
Brazil
|
Level III-2
Some risk of bias
|
L-J solid medium and 7H9 broth culture
|
N=140 sputum specimens from 140 HIV+ patients
|
HIV+ AFB (ZN)
IS6110 qPCR
|
37
41
|
0
1
|
10
6
|
93
92
|
None reported
|
Marchi et al. (2008)
Brazil
|
Level III-2
Some risk of bias
|
L-J-MTBAC culture
|
N=117 sputum specimens
|
AFB (ZN)
IS6110 PCR
|
3
3
|
0
0
|
3
3
|
111
111
|
None reported
|
Ereqat et al. (2011)
Palestine
|
Level III-2
Some risk of bias
|
Culture
|
N=95 samples from 84 patients
n=13
n=82
|
AFB (ZN)
IS6110 PCR
AFB + NAAT
AFB +ve: IS6110 PCR
AFB –ve: IS6110 PCR
|
7
10
10
7
3
|
6
20
20
6
14
|
4
1
1
0
1
|
78
64
64
0
64
|
None reported
|
Shukla et al. (2011)
India
|
Level III-2
Some risk of bias
|
L-J culture
|
N=40 AFB +ve sputum specimens
|
AFB +ve: IS6110 nPCR
|
36
|
0
|
2
|
2
|
None reported
|
Gomez et al. (2011)
Southern Texas (USA) and Mexico
|
Level III-2
Some risk of bias
|
MGIT liquid medium and L-J solid medium culture
|
N=150 patients
-
n=101
n=49
|
AFB
RD1 qPCR
IS1081 qPCR
IS6110 qPCR
AFB + NAAT (IS6110 qPCR)
AFB +ve: IS6110 qPCR
AFB –ve: IS6110 qPCR
|
99
102
102
104
107
96
8
|
2
0
0
0
2
0
0
|
8
5
5
3
0
3
0
|
41
43
43
43
41
2
41
|
32 had contaminated MGIT
|
Haldar et al. (2007)
India
|
Level III-2 Some risk of bias
|
7H9 liquid media culture
|
N=148 sputum samples
|
Direct AFB (ZN)
USP AFB (ZN)
devR FL-PCR
devR Gel-PCR
IS6110 FL-PCR
IS6110 Gel-PCR
|
24
85
111
109
110
102
|
2
2
2
5
4
3
|
96
35
9
11
10
18
|
26
26
26
23
24
25
|
None reported
|