Use the completed data table (illustrations too, if fresh samples are used) for assessment.
Have students identify the kingdoms represented by these organisms:
Humans
Nitella
Rhizopus
Moss
Fern
Ulva
Chlamydomonas
Have students demonstrate the stages and processes in generalized gametic meiosis.
Follow-up/extension
Have students investigate natural and artificially induced polyploidy in plants.
Have students explain how polyploidy can be induced.
Have student relate what goes on in meiosis to Mendel’s Laws of heredity.
Have students model and explain the impact of crossing-over and linked genes, using meoitic processes.
Resources
Suggested Web sites with information on meiosis and gametogenesis:
Meiosis Tutorial. http://www.biology.arizona.edu/cell_bio/tutorials/meiosis/main.html.
Suggested Web sites with information on polyploidy, gametophytes and zygospores:
Algae and Bryophytes. http://www.science.siu.edu/plant-biology/PLB117/Nickrent.Lecs/Algae.bryos.html.
Fertilization, Mitosis and Meiosis. http://waynesword.palomar.edu/lmexer8.htm.
Ploidy. http://www.emc.maricopa.edu/faculty/farabee/BIOBK/BioBookmeiosis.html#Ploidy.
Meiosis — Zygotic, Gametic, and Sporic
Student Activity Sheet
Name: Date:
List the three non-animal eukaryotic kingdoms:
1. Kingdom
2. Kingdom
3. Kingdom
The following organisms may not be familiar to you, with the exception of humans, of course. Research each organism, using the Web sites listed, and fill in the table. Discuss your results.
Data Table
Organism
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Type of Meiosis
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Type of Gametes
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Name of Haploid Cells
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Dominant Phase
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Humans
http://waynesword.palomar.edu/lmexer8.htm
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Nitella
http://www.science.siu.edu/plant-biology/PLB117/Nickrent.Lecs/Algae.bryos.html
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Rhizopus
http://highered.mcgraw-hill.com/sites/0073031216/student_view0/exercise26/life_cycle_of_rhizopus.html
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Moss
http://waynesword.palomar.edu/lmexer8.htm
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Fern
http://waynesword.palomar.edu/lmexer8.htm
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Ulva
http://wps.prenhall.com/wps/media/objects/488/499858/CDA27_1/CDA27_1a/CDA27_1a.htm
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Chlamydomonas
http://highered.mcgraw-hill.com/sites/0073031216/student_view0/exercise24/life_cycle_of_chlamydomonas.html
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| Prokaryota
Activity adapted from “Environmental Sampling of School Sites — A Prediction Activity.” 2004. http://mathinscience.info. Used by permission.
Organizing Topic Investigating Cells
Overview Students investigate prokaryotes and explore the niches they fill in our environment.
Related Standards of Learning BIO.1a, b, c, e, i,; BIO.4a; BIO.5a, b
Objectives
The students will explain the following:
The simplest life forms exhibiting cellular structure are prokaryotes.
Earth’s first cells were prokaryotes.
Prokaryotic cells exist in two major forms: eubacteria and archaebacteria.
Prokaryotes are Earth’s most abundant organisms due to their ability to live in a variety of environments.
Eukaryotes are more complex than prokaryotes and developed into larger more complex organisms, from single-celled protista to multicellular fungi, plants, and animals.
Materials needed
Copies of the attached student activity sheet
Copies of the attached data table
Parts 1 & 2
Nostoc sp. cultures
Prepared slides of eubacteria
Microscope slides
Microscopes, at least one of which has oil immersion
Digital microscope and interface (if available)
Winogradsky column (http://www.accessexcellence.org/AE/AEPC/WWC/1991/microbial.html)
Part 3
Sterile test tubes with stopper, half-filled with deionized sterile water
Aerobic Count (AC) Petrifilm™ plates
Sterile cotton swabs
Sterile micropipette
Petrifilm™ spreader for AC plates
Sealable bags
Pen
Incubator or warm area for incubating Petrifilm™ plates
Biohazard bag for destroying bacteria
Autoclave
Instructional activity Content/Teacher Notes
Prokaryotes are very simple, single-celled life forms. The typical prokaryotic cell includes a circular piece of free-floating DNA called a “nucleoid,” ribosomes, a cell membrane and cell wall, and perhaps a flagellum or other motility apparatus. This is very different from the complex, membrane-bound organelles and defined nucleus of the eukaryotic cell.
Fossils of prokaryotes have been found that are 3.5 billion years old., making today’s prokaryotes descendants of the oldest living inhabitants of Earth. Prokaryotes have evolved with an amazing ability to adapt. They are ubiquitous, found in every conceivable environment. They are found in the deepest of the deep sea trenches, producing their own food from the sulfur vents via chemosynthesis. They survive the highest mountain lakes, the driest deserts, low (0) pH environments, and high pH environments in soda lakes. The prokaryotes that are survivors of these extreme conditions are called “extremophiles.” Scientists have studied the genomes of these extremophiles and have placed them in a domain or kingdom all their own, the Archaea.
Other prokaryotes have been placed in the domain or kingdom Eubacteria (or Bacteria, in some textbooks). These are the bacteria that are commonly found in human environments. Some are beneficial, providing foods such as yogurt and sauerkraut, while others are deadly, like Clostridium botulinum, which causes botulism, and Bacillus anthrasis, which causes anthrax. Most other bacteria simply exist in all human environments, filling their ecological niche as decomposers. (See http://www.ucmp.berkeley.edu/archaea/archaea.html.)
Because they are so efficient as decomposers and relatively simple organisms, science has found more and more uses for the lowly bacterium, e.g., in industry, bioremediation, genetic engineering, and nanotechnology.
There are numerous ways that one can approach the study of bacteria. The activities in this lesson give students an opportunity to use industrial microbiology (yogurt) and environmental microbiology (Nostoc and Winogradsky column) to observe a variety of bacterial types, which have a variety of life histories.
Cautions for this lab
Winogradsky columns. These are wonderful teaching tools to bring microorganisms into a macroview. They are also capable of generating deadly anaerobic bacteria, such as Clostridium botulinum. Do not allow students to examine the contents of a Winogradsky column. If you choose to make and display a Winogradsky column, be aware that you may be growing deadly pathogens and that a very strong and unpleasant odor will be produced.
Unknown bacteria. Microorganisms (bacteria) should always be treated with caution. When plating the unknown sample, do not touch the swab or Petrifilm™ plate with your fingers. Wear gloves during collection and observations of prokaryotes. After plating, clean up work areas, place micropipettes and swabs in bleach solution, and wash hands in hot, soapy water.
Plate incubation. Place inoculated Petrifilm™ plate in sealable bag. Do not seal during the incubation time. Seal bag before examining after incubation time. Do not remove Petrifilm™ plate from bag.
Plate disposal. After examination, place all Petrifilm™ plates in biohazard disposal bag. Autoclave entire biohazard bag at 15 lb/in2 (15 psi) pressure at a temperature of 121ºC for 20 minutes, or take to medical center to deposit with sharps (needles), or dispose materials based on direction from your school division’s chemical hygiene plan.
Introduction
1. Discuss with the students background information on the student activity sheet.
Procedure
Part 1. Blue-Green Algae
1. Direct students in preparing a wet mount of the Nostoc sp. culture.
2. Allow students to examine their sample under multiple magnifications.
3. Have students document their observations.
Part 2. Observation of Prepared Slides of Bacteria
1. Obtain prepared slides of various bacterial types.
2. Allow students to observe the slides under multiple magnifications.
3. Have students document their observations.
Part 3. Prokaryotes in the School Environment
1. Have students follow directions on the student activity sheet.
2. Prepare and incubate a control, using distilled water.
1. Have students make and record observations throughout all parts of the lesson.
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