Cercosporin from Pseudocercosporella capsellae



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Fig. 6. Spectral image of sample peak (bottom line) compared to that of standard cercosporin (top line)

obtained from HPLC analysis of pigment extract from Pseudocercosporella capsellae isolate UWA Wlra-7 in ethyl acetate against standard cercosporin.





Fig. 7. Colour differences of the culture filtrates obtained by three isolates of Pseudocercosporella capsellae for experiment 1. A: culture filtrate obtained from UWA Wlra-7, with high amount of cercosporin, B: Culture filtrate obtained from UWA Wlj-3 and C: Culture filtrate obtained from UWA Wln-9as indicated by its colour and HPLC analysis (Table 3).


Fig. 8. 20 day-old Brassica seedlings inoculated with culture filtrates or live hyphae of Pseudocercosporella capsellae (UWA Wlra-7) grown under controlled environment room at 15°C, 12 h photoperiod and a light intensity of 580 μmol photons m-2 s-1. Cotyledon lesions induced by hyphal-free culture filtrate rich in cercosporin on (A) Brassica juncea Rohini, (D) B. napus Trilogy and (G) Raphanus raphanistrum. Lesions developed by washed live hyphae on cotyledons of (B) B. juncea Rohini, (E) B. napus Trilogy and (H) R. raphanistrum. Cotyledon lesions were also induced by original hyphae on three host species (C) B. juncea Rohini, (F) B. napus Trilogy and (I) on R. raphanistrum.


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