Group 3 class 2



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microbio tbl(1)
microbio tbl(1), If jacob wsa in my family

 3) An 18-month-old baby girl is suspected to have Haemophilus influenzae meningitis. She has not been immunized with the HIB vaccine. A rapid latex agglutination test is performed with the spinal fluid to make a definitive diagnosis. What chemical component in the spinal fluid are we detecting with this assay?

A. IgG antibody B. IgM antibody C. Lipopolysaccharide (LPS) D. Polypeptide E. Polysaccharide capsule

 Ans:- E. Polysaccharide capsule

 Ans:- E. Polysaccharide capsule

 -->The latex agglutination test involves the use of latex beads coated with specific antibody that become agglutinated in the presence of homologous bacteria or antigen. This test is used to determine the presence of the capsular polysaccharide antigen of Haemophilus influenzae in serum or spinal fluid.

4)The Haemophilus influenzae vaccine contains which of the following?

A. Lipopolysaccharide (LPS) B. Live attenuated H. influenzae C. Polypeptide antigens containing D-glutamate D. Polyribitol phosphate antigens E. Teichoic acid F. Toxoids

Ans -D. Polyribitol phosphate antigens

Ans -D. Polyribitol phosphate antigens

-->Encapsulated H. influenzae contains capsular polysaccharides of one of six types (A-F). H. influenzae type B is an important human pathogen with its polyribose phosphate capsule being its major virulence factor. As a result, active immunity is built using polyribitol phosphate antigens (capsular polysaccharide) of H. influenzae.

 5) Cerebrospinal fluid from a spinal tap of a patient complaining of a severe headache, fever, and nuchal rigidity revealed the presence of gram-negative coccobacilli. Further testing revealed growth of the organism on growth factor X and V supplemented chocolate agar, and no hemolysis when grown on blood agar. Which of the following organisms represents the above description

A. Bordetella pertussis B. Haemophilus ducreyi C. Haemophilus haemolyticus D. Haemophilus influenzae E. Haemophilus parainfluenzae

Ans:- D. Haemophilus influenzae

Ans:- D. Haemophilus influenzae

--> H. haemolyticus and H. influenzae are the only organisms listed above that require both growth factors X and V for growth; however, they can be distinguished from each other in that H. influenzae is hemolysis negative on blood agar, whereas H. haemolyticus is hemolysis positive.

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