Control Salmonella Contamination on Egg Shell Surfaces Using Lytic Bacteriophages
Olivia Price and Gabriel Villar Monte Palma Pantoja
Mentor(s): W. T. Evert Ting
Graduate
Peg-board Presentation
Rational: Bacteriophages provide an alternative bio-control for effectively lysing Salmonella bacteria. Phages which lyse their host cells without imposing injury to human cells, can be isolated from raw sewage and propagated in the laboratory.
Objectives: The objective of this study is to determine the effectiveness of two phage isolates, Phage 8 and 2J, to kill Salmonella. The first analysis was to determine the effect of pH and suspension buffer on phage lysis capability; the second, to evaluate the performance of 2J in eliminating Salmonella on egg shell surfaces.
Methods: Phage 8 and 2J were isolated from raw sewage. These isolates were chosen for their ability to lyse at least four serovars of Salmonella. Each isolate was suspended in SM or PBS buffer and mixed with an overnight culture of Salmonella. After 24h, 37˚C incubation, viable counts of Salmonella from each buffer were compared. Each isolate was also suspended in PBS solution at pH values of 5.0, 7.4, or 9.0, and mixed with an overnight culture of Salmonella. Following 24h, 37˚C incubation, viable counts were compared. Additionally, egg shells are artificially inoculated with an overnight Salmonella culture then treated with 2J to determine log reduction values. All tests are performed using a phage to bacteria ratio of 1000:1
Results: Phage 2J consistently demonstrated higher lysis capabilities than phage 8. The performance of 2J was not influenced by pH. Preliminary results for 2J treatment on egg shell are currently ongoing.
Life time Reproductive Success in Nicrophorus Orbicollis
Gopi Patel, Veronica Preito, and Emily Orlando
Mentor(s): Curtis Creighton
Graduate
Peg-board Presentation
Natural selection and other evolutionary forces subject organisms to optimize their fitness (i.e survival and reproduction) under environmental challenges. Since resources are finite and selection cannot maximize fitness beyond certain evolutionary constraints, trade-offs between life history traits such as age, growth, reproduction and lifespan must be made in response to ecological stresses posed by a dynamic environment. In this experiment, life history trade-offs in response to a microbial challenge were examined by focusing on regulation of social immune activity in burying beetle, Nicrophorus orbicollis, in response to manipulation in food quality and microbial activity in their surrounding environment. Female burying beetles were exposed to a microbial environment different than one they were raised on to raise their offspring, their longevity, size, mass and fecundity were measured to determine their life time reproductive success. N. orbicollis is a model organism for such a study because it provides biparental care to their offspring and is known to elicit an immune response to protect their offspring which can be quantified to measure fitness. Understanding regulation of expression of immune molecules can allow us to understand gene regulation in terms of inheritability and tradeoffs in N. orbicollis and which can be utilized to learn such regulatory mechanisms in higher organisms.
Visualization of Ternary Phase Diagram Using Mobile Applications and 3D Printed Models
Huanan Shen
Mentor(s): Harvey Abramowitz
Graduate
Peg-board Presentation
Visualization of ternary phase diagrams is an ongoing endeavor at Purdue University Calumet. In this work, the visualization efforts were extended to include mobile applications and physical models made by 3D printing. Visualizations focused on the liquidus surfaces of ternary alloy phase diagrams. To generate these surfaces an interpolation method, based on Ternplot code, was used. These surfaces can then be visualized in a number of ways on mobile devices. The mobile device can view the surfaces through a web application which allows them to be rotatable. An augmented reality app allows another type of 3D visualization. In addition to the mobile applications, 3D printing produced physical models which are easy to manipulate. To demonstrate these methods of visualization, six ternary alloys were selected: Cu-Sn-Pb, Cu-Sn-Ag, Ag-Sn-Sb, Sb-Sn-Pb, Pb-Sn-Bi, and Bi-Sn-Sb. These alloys form a hexagonal carousel with Sn being the central connecting element.
Xia Chen and Mohammad Islam
Mentor(s): Chen Zhou and Guangwu Tang
Graduate
Peg-board Presentation
Reheating furnaces are commonly used in steel rolling process, which are the critical components in steel slab production. These furnaces have an important impact on both steel quality and total cost. With the purpose of obtaining a better understanding of the furnace operation, a Computational Fluid Dynamics (CFD) model has been developed to examine furnace operation. The CFD model has been validated with the industry experiment. The model can be further used to analysis the through-thickness temperature gradient with a slab, the proper residence time, the curved skid riders’ influence on the skid marks, etc. Also, a series of simulations have been conducted to optimize the furnace performance. The comparison of the results helps for understanding the effective factors which are significant to the improvement of the furnace’s production ability, thus providing insight into furnace optimization.
CFD Modeling of a Ladle with Top Stirring Lance
Xiaoxiao Zhang and Xia Chen
Mentor(s): Chenn Zhou, Bin Wu, and Guangwu Tang
Graduate
Peg-board Presentation
Stirring ladles have been widely used in steelmaking industry. Steel cleanliness is very important for the quality of final products. Proper stirring is crucial for obtaining the clean steel. In this study, a 3D computational fluid dynamics (CFD) model has been developed to simulate the stirring process in a ladle. A stirring lance is immersed into the hot metal to conduct a consistent stir. The computational model provides an insight view of the hot metal, slag and gas phase interactions. The simulation results have been validated by the industrial experiment. A mixing study has been conducted to monitor the mixing process. Parametric studies have been accomplished to numerically investigate the mixing time with different operating conditions to optimize this process and improve the efficiency of the stirring process. / Keywords: stirring ladle, hot metal, CFD, multiphase flow, mixing process /
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