5 – 27. Department of Transportation testing procedures and required education and training a. Civilian drivers to whom DOT rules apply are subject to the testing procedures identified in 49 CFR Part 40. b. The DOT regulations require supervisor training and driver education. Requirements are in 49 CFR 382.601 and 382.603. 5 – 28. Department of Transportation frequency of random alcohol and other drug testing Random testing of drivers for alcohol and other drugs will occur at the minimum rates published in the Federal Register annually.
