Ayman Ahmed Abedallah Elshayeb Ph. D



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Ayman Ahmed Abedallah Elshayeb Ph.D. Microbiology, University of Khartoum

University of Khartoum, Faculty of Science, Department of Botany, Zip Code:11115,

Khartoum - Sudan

: ayman_elshayeb@yahoo.com



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Nationality: Sudanese

Date of Birth: 25th April 1971

Language:

Arabic: (mother tongue).

English: (spoken + written) fluent.



German: (spoken + written) very good.
Profile: As a virologist and academic lecturer, I have been working on viruses that are related to human and health issues. Relatively, I have been interested in the development of the bacteria resistance against chemotherapeutic agents that is becoming an increasing problem specially the Multi Drugs Resistant Bacteria (MDR), and this had the potential to facilitate more rational thinking about the Bacteriophages’ therapy for the increasing incidence of bacteria resistant against most antibiotics. I have been working in scientific research with similar challenges using my experience to help work anticipate and respond more quickly and effectively.

Objectives: The main objectives are; managing the applications of Bacteriophage conceptions as integrated therapeutically framework. These include; specific knowledge of virological research administration, lows regulations, problems analysis, evaluation of issues that support economic progression in the section, achievement of the work and priorities towards the protection of microbiological and sustainable development and other related duties as required.

Career: Virologist PhD: Microbiology in general and Virology for particular specialization

Professional Experience:


  • December 2014 up to date: Virologist Researcher: Karary University:

Duties: conduct research pertaining to viruses, working on new vaccines, in the field searching for new treatments, responding to viral outbreaks. Teach and supervise medical graduate students. Participate in the following programs:

1- Research Unit: - Develop strategies for effective working to undertake translational clinical research. Promote scientific and clinical concerns of researchers' work through guidance and monitoring of research health projects research in Sudan. Ensure that submitted projects meet human ethics through reviewing them by the Medical Research Unit Ethics Committee and rules. Ensure the establishment of health research data base, and publication of scientific and applied findings of health research projects.

2- Central Research Laboratory: Established by the endorsement of the research unit where the involved student taking an investigative project under the supervision of an academic staff. The C.R.L offers a set of services ranging from support to the design and implementation of scientific projects to the provision of custom services in medical field.

  • June 2014 up to date: Environment and microbial Consultant: Tahir Consultancy Flares: Duties: Initial and feasibility studies of environmental and microbial impact of fisheries. Technical studies, planning, design and preparation of detailed documents. Evaluate the performance of the facilities and determine its financial position. Assess existing projects financially and technically oversee the implementation of projects.

  • December 2012 Up to date: PhD Student: University of Khartoum, Department of Microbiology: Research work: Potentiality of Bacteriophage’s Lytic Enzymes as antimicrobial agents against Ciprofloxacin Resistant Salmonella Typhi

  • January 2010 – June 2013: Part-time Lecturer, Al-Neelain University, Faculty of Sciences and Technology, Dept. of Biology: I have been teaching general aspects of microbiology, molecular biology and immunological diagnostic techniques for under graduates. I have also taught research methodology and supervised three undergraduate students in their projects.

  • February 2005— Marsh 2009: Part-time Teaching Assistant, Al-Neelain University, Faculty of Sciences and Technology, Dept. of Biology

  • In lab teaching, systematic bacteriology, bacteriophage isolation, molecular techniques and proteomics.

  • February 2005— Marsh 2008: Postgraduate Student, Al-Neelain University, Faculty of Science and Technology, School of Biotechnology, Department of Microbiology and Molecular biology

  • Research: Estimate the bacterial load of Soba Wastewater Stabilisation Station. Isolation of Escherichia. coli and Staphylococcus aureus and their bacteriophages from the Station. In vitro tests of the isolated bacteriophages against Escherichia. coli and Staphylococcus. aureus isolated from sewage.

  • February 2005— Marsh 2008: Postgraduate Student, Tropical Medicine Research Institute, National Research Centre, Ministry of Sciences and Technology. Carry out the molecular aspects of the research. Determination of protein profiles of bacteriophages and their corresponding bacteria by using Sodium Dodocyl Sulphate Polyacrylamid Gel Electrophoresis (SDS-PAGE).‎Examining the bacteriophage efficiency against local bacteria species and comparing and measuring the bacteriophage efficiency with the antibiotics susceptibility.

  • January 1998— July 1998 Graduate Student: Fisheries research centre, National Research Centre, Ministry of Sciences and Technology, Khartoum, Sudan. Controlling three Nile fish aspects and their biological and microbiological interactions in artificial habitat, food and feeding mechanisms and measuring the breeding behaviour.

Ongoing research projects:


1- Bacteriophage Therapy: 2005 – Up to date: This project was conducted to develop Bacteriophage Therapy technology platform including novel methods for the targeted delivery of therapeutic bacteriophages by isolating and identifying bacteriophages from local environment to be used as antimicrobial therapy against certain antibiotics resistant bacteria species.

2- HIV-1 in Sudan: 2014 – Up to date: The aim of this project is to determine the HIV-1genotype and subtypes that cause AIDS in Sudan and the relation between subtypes and opportunistic infection, as well as the effect of antiretroviral drug on HIV/AIDS patients are also looked into and determine the effect of some antiretroviral drugs for those patients who are on treatment.

3- Hemorrhagic Fevers Viruses in Sudan: 2014 – Up to date: The objective of this project is to study the sero-prevalence, genoypes and epidemiology of Arboviral Hemorrhagic Fevers Viruses in Sudan. Considering raising threat of emerging hemorrhagic fevers to national security as well as economical influences due to affection of livestock, it is mandatory to build strong surveillance system targeting arboviruses to enable early detection, prompt control and prevention

Skills and Accomplishments:


  • Microbiology Lecturer 2009 - 2011

  • Teaching Assistant: 2005 -2009 •Undergraduate courses School of Life Sciences Al-Neelain University

  • Protein separation Tutor: 17-27 /2/2007 • Advanced course of molecular biology. First winter course, School of Life Sciences Al-Neelain University

  • Bioinformatics Tutor: 17-27 /2/2007• Advanced course of molecular biology. First winter course, School of Life Sciences Al-Neelain University

  • Techniques Lecturer: 5-10 /1/2008 School of Life Sciences in collaboration with the International Union of Immunological Societies and The Federation of African Immunological Societies.

  • Facilitated the Blue Print assessment for Medical Student 2013. Bahri University, College of Medicine, Pediatrics Department, Khartoum – Sudan.

Virology skills:

  • Serological detection, RT- PCR for Geno-typing, Flow cytometry for CD4 counting, cell-cell fusion assay, viral-cell fusion assay, infectivity assay and generation of pseudo-typed.

Immunology skills:

  • Tube precipitation test; Gel diffusion, Single radial and Double radial (Ouchterlony). Agglutination tests; ABO Rapid Diagnosis using card kits – Malaria, Trypanosomiasis and other parasites. Monoclonal antibody production, antibody purification, antibody isotyping, enzyme-antibody conjugation, sandwich ELISA development, immunohistochemical, staining, and isolation of murine immune cells (splenic T-lymphocytes and B-lymphocytes, peritoneal macrophage, and bone marrow cells), mixed lymphocyte reaction.

Molecular Biology skills:

  • Plasmid preparation, ligation, transformation, Genomic DNA extraction, total RNA extraction, RT-PCR, PCR, site-directed mutagenesis, manual and automated DNA sequencing, microarray.

Protein skills:

  • Protein purification, Thin layer, GC and HPLC FPLC, HPLC (affinity, gel filtration, ion exchange and reverse phase), preparative column chromatography, membrane filtration, dialysis, SDS-PAGE, Western blot, bacterial protein expression, protein refolding, mammalian cell protein expression, Biosensor (IAsys) operation, and ELISA.

Techniques:

  • Bacteriophage Assay

  • HIV-1 detection and antiretroviral resistance

  • Hemorrhagic Fevers Viruses epidemiological and prediction systems.

  • Bacteriological procedures like staining, culturing of clinical specimens

  • Fluorescent staining, Acid fast staining and culturing of Mycobacteria

  • Fungal culture and detection of parasites from different specimens

  • Antibiotic sensitivity testing

  • API-testing for various organisms

  • Detection of anaerobes

  • Quality control, sterility checks, and microbial water culture

  • International Organization for Standardization (ISO 9000, 14000), routine work with ISO-6579: 2002 and ISO/IEC 17025.



Computational Skills

  • Operating systems: Windows and Linux

  • Productivity Software: All MS Office Tools

  • Scientific Tools used: Sigma Plot, Statistics data analysis and molecular biology software.

  • Biological Modeling using MATLAB

  • Familiarity with bioinformatics and web-based microbiology and molecular biology services

Education:


  • PhD. Microbiology 2013- 2015 : University of Khartoum, Faculty of Science, Dept. of Microbiology, Khartoum, Sudan

  • M.Sc. Microbiology 2005— 2008 : Al-Neelain University, Faculty of Science and Technology, School of Biotechnology, Department of Microbiology and Molecular biology, Khartoum, Sudan

  • B.Sc. Honour 1994— 1998:Al-Neelain University, Faculty of science Department of Biology, Section Zoology, Khartoum, Sudan.

  • Computer Diploma 1998— 1999 : Khartoum University, Faculty of Economic & Social Studies, Development Studies and Research Centre Khartoum, Sudan.

  • German Language : 1996— 1997: Grundstufe I, II, Goethe Institute & the Sudanese German Cultural Society Khartoum, Sudan.

International Courses

  • Attend training course on ˝Bio-evaluation of Medicinal and Aromatic Plants for Human and Animal Health for African Countries”. June 2008 by the International Centre for Science and High Technology of the United Nations Industrial Development Organization ICS-UNIDO and the Faculty of Veterinary Medicine, University of Pretoria, South Africa

  • Attend training course on “Hands-on research on complex systems”. August 2010 by the Abdu Salalm International Centre for Theoretical Physics (ICTP), U.S National Science foundation and the University of Buea Cameroon.

Awards


  • B.Sc. Honour Al-Neelain University, 1998

  • The Academy of Sciences for the Developing World – Regional Office for Sub Saharan Africa, Travel Award, 2006

  • The Bill and Melinda Gates Foundation Global Health & Keystone Symposia Travel Award, 2008

  • International Centre for Science and High Technology United Nation Industrial Development Organization Travel Award, 2008

  • African Biotechnology Stakeholder Travel Award, 2008

  • The International Society of Urban Health & the Bill and Melinda Gates Foundation Global Health Travel Award, 2009

  • The Abdu Salam International Centre for Theoretical Physics, Travel Award, 2010

  • National Institute of Allergy and Infectious Diseases & National Institutes of Health United State of America, Travel Award, 2011

  • Qatar Foundation for Education Science and Community development Travel Award 2012.

International conferences attending and participations: -


  • The 1st TWAS - ROSSA Young Scientists' Conference in Africa, 2006 Nairobi Kenya. Topic (Protein profile of Soba Stabilisation's Bacteriophage and Their Bacterial Hosts' Proteins).

  • The 3rd Federation of European Biochemical Societies. Advanced Lecture Course on Systems Biology 2009, Alpbach, Austria. Topic (Identification of protein profiles of Escherichia coli, Staphylococcus aureus and their corresponding phages).

  • Keystone Symposium “Translating new technologies to improve public health in Africa”, 2008 Uganda. Topic (A comparative study of the Bacteriophage Efficiency and antibiotics susceptibility against Sudanese local bacteria species Escherichia coli and Staphylococcus aureus).

  • The 8th International conference of urban health, 2009 Nairobi Kenya. Topic (The Role of Bacteriophage Associated with Soba Stabilisation Station Efficiency in Bacterial Removal).

  • International Society for Computational Biology (ISCB) and the African Society for Bioinformatics and Computational Biology (ASBCB) Joint Conference on Bioinformatics of Infectious Diseases, 2009, Bamako Mali. Topic (Bioinformatics applications for Bacteriophages and their bacterial hosts’ proteins analysis).

  • National Institute of Allergy and Infectious Diseases & National Institutes of Health U.S.A conference on Bacterial Waterborne and Emerging Infectious Diseases in North Africa and the Middle East: Opportunities for Collaboration, 2011 Nicosia, Cyprus.

  • European Conference on Health Economics, Zurich Switzerland 2012. Topic: The Effects of Climate Changes on the Spread and Distribution of Malaria in Sudan, http://eche2012.abstractsubmit.org/presentations/2551/.

  • The 6th International Conference on Environmental Mutagens in Human Populations 26th - 29th March 2012, Doha - Qatar.

  • Society for General Microbiology Spring Conference 2012 Convention Centre Dublin - Ireland. http://www.sgm.ac.uk/meetings/SGM%20Dublin%202012_Abstracts.pdf.

  • TAAG First African Student’s Conference, Nairobi. 2-4 July, 2013, http://www.tropical-biology.org/information/admin/Documents/Book%20of%20Abstract-TAAG%20Nairobi%202013.pdf.

  • The 2nd International Congress on Pathogens at the Human-Animal Interface (ICOPHAI):One Health for Sustainable Development August 14-17, 2013 Porto de Galinhas, Brazil, http://www.icophai2013.org/final-program .

  • The 6th MIM PAN African Malaria Conference Durban, South Africa, http://mim2013.mrc.ac.za/BoAposter_final.pdf.

  • Qatar Foundation Annual Research conference 22 Nov 2013, Doha – Qatar, http://www.qscience.com/doi/abs/10.5339/qfarf.2013.BIOSP-02?af=R.

  • The 4th World Congress on Virology October 06-08, 2014 Hilton San Antonio, USA, http://omicsonline.org/1948-5964/1948-5964_Virology-2014_Accepted-Abstracts.digital/

Editor and reviewer of international journals:


  • Regional Editor of American Journal of Science hub http://www.scihub.org/index.html

  • Member of Asian Council of Science Editors,

http://theacse.com/newmembers.php?pageNum_Recordset1=25&totalRows_Recordset1=308

  • Member of Editorial board of ADVANCES IN BIORESEARCH [A Biannual Peer Reviewed International Journal of Life Sciences] [PUBLISHED BY: SOCIETY OF EDUCATIONAL, AGRA U.P. INDIA]. http://soeagra.com/abr_editorial.htm

  • Member of Editorial board of Research Journal of Chemical and Environmental Sciences. {Bi monthly Referred /Peer Reviewed International Journal of Chemical and Environmental Sciences}. {Published By: Academy for Environment and Life Sciences, Agra, U.P. INDIA}. http://www.aelsindia.com/editorial.htm.

  • Reviewer of the Scientific and Technical Committee & Editorial Review Board on Biological and Life Sciences Journal http://www.waset.org/BiologicalandLifeSciences.php?page=9 .

  • Reviewer of the Bulletin of the World Health Organization 2013. http://www.who.int/bulletin/volumes/91/12/reviewers1213.pdf?ua=1

  • Reviewer of the African Journal of Microbiology Research 2013

  • Reviewer of the British Microbiology Research Journal 2014

  • Editor of Saudi Journal of Biological Science 2014 . http://ees.elsevier.com/sjbs/default.asp

List of Publication


Books

Elshayeb A.A., Ygoub, S.O and Elagib, A.A., (2010). IN VITRO TESTS FOR BACTERIOPHAGES AGAINST SUDANESE BACTERIA SPECIES. Bacteriological Studies and Protein Profiles of Escherichia coli, Staphylococcus aureus and Their Phages That Isolated From Soba Sewage Station. ISBN-NR. 978-3-8383-8603-4: LAMBERT Academic Publishing AG & Co. KG Saarbrücken Dudweiler Landstraße 99, 66123 Saarbrücken Germany.

Elshayeb A.A. and Nasr A.O., (2010). World Malaria Day Symposium 2010. Theme: Malaria Elimination in Sudan (25th of April 2010). Abstracts’ book. © Al-Neelain Medical Research Center (AMRC), Faculty of Medicine & Health Sciences, Al-Neelain University, Khartoum, Sudan.

Journal Articles

Elshayeb A.A. (2010). The impact of bacteriophages in bacteria removal associated with soba stabilisation station efficiency. African Journal of Microbiology Research Vol. 4 (4), pp. 233-239, 18 February, 2010 ISSN 1996-0808 ©2010 Academic Journals. Available online http://www.academicjournals.org/ajmr

Elshayeb A.A. Ashraf S. Yousif; Eshtiyag A. Abedalkareem; Salma M. El hag Ygoub, S.O ,Elagib, A.A., (2011). Identification of Protein Profiles for Escherichia coli, Staphylococcus aureus and their Corresponding phages, AMERICAN JOURNAL BIOTECHNOLOGY AND MOLECULAR SCIENCES ISSN Print: 2159-3698, ISSN Online: 2159-3701, doi:10.5251/ajbms.2011.1.2.39.44 © 2011, ScienceHuβ, http://www.scihub.org/AJBMS

Elshayeb A.A. Elagib, A.A., Ygoub, S.O (2011). The Bacteriophage efficiency and antibiotics susceptibility against Sudanese local bacteria species Escherichia coli and Staphylococcus aureus. AMERICAN JOURNAL OF SCIENTIFIC AND INDUSTRIAL RESEARCH © 2011, Science Huβ, http://www.scihub.org/AJSIR ISSN: 2153-649X doi:10.5251/ajsir.2011.2.5.718.725.

Elshayeb Ayman. (2013). Bioinformatics applications for Bacteriophages and their pathogenic bacteria proteins Analysis. Qatar Foundation Annual Research Forum Proceedings: Vol. 2013, BIOSP 02. DOI: 10.5339/qfarf.2013.BIOSP-02 .Published online: 22 Nov 2013. http://www.qscience.com/doi/abs/10.5339/qfarf.2013.BIOSP-02

Elshayeb A.A., Ahmed A.A and EL-Hussien A.A., (2014). Modelling the prediction of bacteriophage potentiality as antimicrobial agent against Salmonella Typhi. (submitted).

Elshayeb A.A., Mujtaba M.I, Ahmed A.A and EL-Hussien A.A., (2014). Influence of seasonality on Salmonella concentration and dynamics in Khartoum wastewater stabilization station. (In press).

Elshayeb A.A., Ahmed A.A and EL-Hussien A.A., (2014). Ciprofloxacin susceptibility pattern and future trends against Salmonella Typhi in Sudan. (In press).

Abdelaal R. and Elshayeb A.A, (2011). The effects of climate changes on the distribution and spread of malaria in Sudan, American Journal of Environmental Engineering. (1):15-20 DOI:10.5923/j.ajee.20110101.03. http://article.sapub.org/10.5923.j.ajee.20110101.03.html

Salih K.M.A, Saeed, E.S, Karsani, M.S, Elshayeb, A.A and Ibrahim S.A., (2010).Pattern of bacterial meningitis in Sudanese children, Omdurman, Sudan, ISSN 1996-0808 © 2010 Academic Journals African Journal of Microbiology Research Vol. 4(21). http://www.academicjournals.org/ajmr

Karsani, M.S, Elshayeb, A.A and Salih K.M.A., (2012). Epidemiology and recent outbreak of cholera in Sudan. ADVANCES IN BIORESEARCH .Volume 3, Issue 1, March 2012: 23 – 25 ISSN 0976-4585 © Society of Education, India. Website: www.soeagra.com/abr.htm

Mubarak S. Karsany, Ayman A. Elshyeb, El Nageeb S. Saeed, Rehab Elaagib, Salah A. Ibrahim, Elfatih Elsamani, Kamal Hussien and Karimeldin M. Salih, (2013). Patterns of meningococcal infections in Sudan with a new emergence of Niesseria meningitidis W135. (3 EMHJ Eastern Mediterranean Health Journal, EMHJ • Vol. 19 No. 10 • 763-766. http://www.emro.who.int/emhj-vol-19-2013/10/patterns-of-meningococcal-infection-in-sudan-with-emergence-of-neisseria-meningitidis-serogroup-w135.html

Abdelrahman, A., Gamil., Karimeldin M., A., Salih and Ayman., A., Elshayeb, (2013). Resuscitation of traumatic brain injured Sudanese patients. AMERICAN JOURNAL OF MEDICAL AND DENTAL SCIENCES. ISSN Print: 2326-8638, ISSN Online: 2326-8648, doi:10.5251/ajmds.2013.1.2.35.39 © 2013, ScienceHuβ, http://www.scihub.org/AJMDS

Ali A. Mohammed, F. Idris Omar, and Ayman A. Elshayeb. (2014). The hazardous impacts of mining activities on population's health in Blue Nile State - Sudan. Journal of Local and Global Health Science: Vol. 2014, 3. DOI: 10.5339/jlghs.2014.3 Published online: 16 Jul 2014. Read More: /doi/abs/10.5339/jlghs.2014.3 http://www.qscience.com

Mohammed A. Hammad, Karimeldin M.A. Salih, Mohammed A. Abdallah, Rashid A. Salih, Ahmed A. Mohammedani, Isam M. Elkhidir, Ayman A. Elshayeb., (2014). Determination of Human Immunodeficiency Virus Type 1 and Allied Subtypes in Sudan. Int J Cur Res Rev | Vol 7 • Issue 5 • M arch 2015. http://www.scopemed.org/?mno=183133

Mubarak S. Mustafa, Rehab Elagib, Mutasim M. Mangory , Karimeldin A. Salih, Ayman A. Elshayeb, (2011). H1N1 outbreak in paediatric hospitals in Khartoum State- Sudan. (Submitted for publication)

Abdelrahman, A., Gamil., Karimeldin M., A., Salih and Ayman., A., Elshayeb, (2013). Uncommon intracranial ependymoma in one year old child First case reported in Sudan

Nimr Y. S., Elshayeb A.A., and Aboud M. A., (2013). Estimation of Actual malaria transmission in central-east of Sudan. (In press).



Professional and Community Memberships


  • Member of the Sudanese Biologists Association

  • Member of the Sudanese Young Scientists Society

  • Participant of HIV Project in collaboration with Sudanese Biologists Association and ACORD organization

  • E-affiliate member of the Society for Applied Microbiology (SFAM) United Kingdom.

Member of the World Association of Young Scientists http://ways.org/fr/user/elshayeb

Referee


Referee available upon request.



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