Marshall s. Mcgoff

marshall.mcgoff@gmail.com

425-344-2591
OBJECTIVE:

To obtain a job in the Chemical industry

Research Assistant II in Hackney Research Group, Feb 2013 – present

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  Expressed kinesin-1 protein mutants in E. coli. and purified using several different affinity tags for ease in chromatography and increased solubility (His-6 tag, Maltose Binding protein fusion respectively)
  
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  Ran ATPase assay on mutants by observing the consumption of NADH via UV-Vis spectroscopy
  
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  Checked protein aggregation via Gel Filtration Chromatography and subsequent Dynamic Light Scattering
  
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  Ran SDS-PAGE gels to assess purity of protein samples
  
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  Assessed protein secondary structure and stability via circular dichroism
  

Graduate Research Assistant in the Saxena Research Group, Mar 2011 – Dec 2012

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  Mutated with PCR, expressed in E. coli, purified using FPLC, and characterized using LCMS the B1 immunoglobulin-binding domain of protein G (GB1)
  
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  Used Electron Spin Resonance to analyze spin labeled GB1 structure and dynamics
  
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  Crystallized spin labeled GB1 (lead to publication below)
  
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  Devised unique research project independently
  

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  Responsible for 15 undergraduates per section per semester
  
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  Instructed students on background information critical to understanding of instrumentation and the purpose of the lab experiment
  
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  Tested new experiments, assisted with implementation of new instrumentation accompanying the modernization of the lab
  
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  Ensured instrumentation was operational and (GCMS, FTIR, ICP-AES, HPLC, Autotitrator)
  
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  Supervised independent projects in which students designed their own experiments, graded PowerPoint presentations, and helped organize poster session in lieu of a final lab exam
  

Analyst I, QC Raw Materials, Aug 2008 – Jul 2010

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  Performed raw material testing using compendial methods (USP/NF, ACS)
  

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  Incorporated laboratory results into SAP and LIMS
  
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  Developed GC methods using Empower software to bring method in-house and reduce dependency on contract labs
  
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  Revised and drafted new SOPs to reflect new changes in compendial methodology as well as new instrumentation in the lab
  

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  2011 Safford Award for Excellence in Graduate Student Teaching from the University of Pittsburgh’s Department of Chemistry
  

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  Cunningham, T. F.; McGoff, M. S.; Sengupta, I.; Jaroniec, C. P.; Horne, W. S.; Saxena, S., High-Resolution Structure of a Protein Spin-Label in a Solvent-Exposed beta-Sheet and Comparison with DEER Spectroscopy. Biochemistry 2012, 51 (32), 6350-9
  

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  Treasurer, Phi Lambda Upsilon (2011-2012)
  
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  Member, Society of Analytical Chemists of Pittsburgh (2011-present)
  
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  Member, Spectroscopy Society of Pittsburgh (2012-present)