Supplemental Material Carbohydrate utilization



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C. Adheins and outer membrane proteins



Gene

H. somnus 129Pt

H. ducreyi 35000HP

H. influenzae Rd

H. somnus autotransporters










possible large adhesin

HS_0209

no


no

possible large adhesin

HS_0383

no

no

possible large adhesin

HS_0478

no

no

possible large adhesin

HS_0589

no

no

possible large adhesin

HS_0602

no

no

possible large adhesin

HS_0790

no

no

possible large adhesin

HS_1058

no

no

possible large adhesin

HS_1085

no

no

possible large adhesin

HS_1154

no

no

possible large adhesin

HS_1234

no

no

Autotransporter adhesin

HS_1543

no

no

possible large adhesin

HS_1616

no

no

possible large adhesin

HS_1632

no

HI1718

periplasmic zinc transporter/adhesin B precursor

HS_1256

HD0198


HI0119





no

HD0281

major pilin protein precursor/adhesin B precursor



no

H. influenzae adhesins










hmw1A

HMW1


no

no

no

hmw2A

HMW2


no

no

no

hia

no

no

no

hsf

no

no

no

hap

no

no

no

H. ducreyi autotransporters










dsrA



HS_1543

HD0769

no

ncaA

no

HD1920

no

Outer membrane proteins










oapA

HS_1287

HD0651

HI0330

oapB

no

HD0652

HI0331

P2

no

HD1433

HD1435


HI0139

P1

HS_1548

HD1078

HI0401

P4

no

HD1170

HI0693

P5

HS_1035

HD0045
HD0046

HI1164


P6

HS_0263

HD1772

HI0381

Table S12. Genes for iron transport and utilization



Gene

H. somnus 129Pt

H. ducreyi 35000HP

H. influenzae Rd

TonB system










tonB

HS_0689


HD0327

HI0251


exbD

HS_0690

HD0328

HI0252

exbB

HS_0691

HD0329

HI0253

Fe-S cluster metabolism










cyaY

HS_1712

N-terminal



HD0473

HI0727.1

N-terminal



Fur










fur

ferric uptake regulation protein



HS_1211

HD0367


HI0190


Heme biosynthesis










gltX

glutamyl-tRNA synthetase



HS_0456

HD0320


HI0274


hemA

glutamyl-tRNA reductase



HS_0810

no

no

hemB

5-aminolevulinate dehydratase (porphobilinogen

synthase)


HS_0548

no

no

hemC

hydroxymethylbilane synthase (porphobilinogen deaminase)



HS_0044

no

no

hemD

uroporphyrinogen-III synthase



HS_0045

HD1742



no

hemE

uroporphyrinogen decarboxylase



HS_1540

no

no

hemF

coproporphyrinogen III oxidase



no

no

no

hemG

protoporphyrin flavoprotein oxidase



HS_1569


no

no

hemH

ferrochelatase



HS_1047

no

HI1160


hemL

glutamate-1-semialdehyde aminotransferase



HS_1229

no


no


hemM (lolB)

outer membrane lipoprotein LolB precursor



HS_0998

HD1629


HI1607


hemN

coproporphyrinogen oxidase, oxygen-independent (coproporphyrinogen-III oxidase)



HS_0472

HD1668
HD0037

this one may be a paralog



HI0463


hemR

hemin receptor, outer membrane protein



HS_0728

HD0388


HI0113


hemX

uroporphyrin-III C-methyltransferase



HS_0046

HD1741



HI0603


hemY

porphyrin biosynthesis protein



HS_0047

HD1740


HI0602


cyoE

protohaeme IX farnesyltransferase



no

no

no

cysG

siroheme synthase



no

no

no

aroB

3-dehydroquinate synthase



HS_1114

HD0422


HI0208


Transferrin binding proteins










tbpA

transferrin-binding protein A



HS_0097*

C-terminal region



no


HI0994




HS_0098*

central fragment



no






HS_0102*

N-terminal region



no




tbpB 

transferrin-binding protein B



HS_0448


no

HI0995

tbpA  

transferrin-binding protein A



HS_0449


no

HI0994

tbpA

transferrin binding protein A



HS_0582



no

HI0994

transferrin-binding protein, putative

no


no


HI1217

Iron-regulated proteins










iron-regulated outer membrane protein

HS_0069


no

no

iron-regulated outer membrane protein

HS_1306


no

no

Heme utilization proteins










heme utilization protein hugZ

HS_0722

HD0620

no

heme utilization protein

HS_0723

no

no

tdhA

TonB-dependent heme receptor A



no


HD0388

no


hxuC

heme-hemopexin utilization protein C



no

no

HI0262


hxuB

heme-hemopexin utilization protein B



no

no

HI0263


hxuA

heme-hemopexin utilization protein A



no

no

HI0264


Hemoglobin binding proteins










hgbB

no

no

HI0635

20 CAAC repeats



hgbA

HS_0721 N-terminal

HS_0720 C-terminal

no repeats


HD2025

no repeats



HI0661

20 CAAC repeats near beginning of gene



hgbB

no

no

HI0712

36 CAAC repeats near beginning of gene



hemoglobin binding protein

no

no

HI1565

18 CAAC repeats near beginning of gene



TonB-dependent receptor, putative

no

no

HI1567


Iron transporters

15 total

9 total

17 total

Chelated iron ABC










yfeD

HS_0325

HD1024

HI0359

yfeC

HS_0326

HD1025

HI0360

yfeB

HS_0327

HD1817

HI0361

yfeA

HS_0328

HD1816

HI0362

Heme ABC










ccmA

HS_0393

HD0786

HI1089

ccmB

HS_0394

HD0787

HI1090

ccmC

HS_0395

HD0789

HI1091

ccmD

HS_0396

HD0790

HI1092







HD0215

HI0853

Fe3+ /Iron III ABC










afuC

HS_0639

no

HI0126

afuB

HS_0640

no

HI0129

afuA

HS_0641

no

HI0131

afuA

HS_0643

no

no













hitA/fbpA

HS_0783

no

HI0097

hitB/fbpB

HS_0782

no

HI0098

hitC/fbpC

HS_0781

no

HI0099
















no

no

HI1470




no

no

HI1471




no

no

HI1472


* These fragments represent the H. somnus 129Pt counterpart of tbpA2 from H. somnus strain 649 and tbpA from H. ovis.


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