I am a biochemist with expertise in molecular biology and biochemistry techniques.
Mechanistic and stereochemical studies on α-oxoamine synthases and related enzymes.
Determination of three-dimensional structure of novel bacterial globin-coupled-sensors, fatty acid desaturase 2 and polyhydroxyalkanoate synthase.
Functional analyses of these proteins.
Analyses of commercially important secondary metabolites and enzymes/proteins from autotrophic and extremophilic organisms.
Develop novel assay methods for enzyme characterisation and kinetic studies.
Cloning, expression and purification of proteins by using FPLC and HPLC.
Kinetic analyses of different enzymes by using UV and mass spectrometry (MALDI-TOF) and radiolabeling techniques.
Experience in crystallisation of different proteins by using sitting drop and hanging drop vapour diffusion techniques.
Elucidation of three-dimensional structures of some proteins, I have used Rigaku MicroMax-007 HF X-ray generator (λ = 1.5418 Å) equipped with a Raxis-IV++ area detector.
I have used several software for processing a diffraction data set and refinement of 3D structure such as Crystal Clear, Refmac, Phenix and Coot.
Trained in DNA microarray.
Ayesha Mazhar, Farrukh Jamil, Qamar Bashir, Misbah Masood, Imrana Tanvir, Naeem Rashid, Abdul Waheed, Muhammad Akram Tariq (Accepted) Genetic variants in FGFR2 and TNRC9 genes are associated with Breast Cancer risk in Pakistani women. Molecular Medicine (In press)
Fizza Tariq, Qanita Khaid, Arslan Sehgal, and Farrukh Jamil (2016) Possible roles of the pseudogenes of Salmonella Typhimurium. Pak. Journ of Zoology. (In press)
Farrukh Jamil (2015) Kinetics of the exchange reactions of 2-mino-3-ketobutyrate CoA ligase. Frontiers in Biology (Springer)
Sonia Kanwal, Farrukh Jamil, Ahmad Ali and Sheikh Arslan Sehgal (2016) Comparative Modeling and Revealing of Potential Binding Pockets. Interdisciplinary Sciences: Computational Life Sciences
Qin Huang, Awais Ihsan, Pu Guo, Xun Luo, Guyue Cheng, Haihong Hao, Dongmei Chen, Farrukh Jamil, Yanfei Tao, Zonghui Yuan (2015) Evaluation of toxic potential of the main metabolites of Cyadox in acute, sub-chronic study and genotoxicity test. Reg. Toxicity and Pharmacology
Farrukh Jamil, Aik-Hong Teh, Ermin Schadich, Jennifer A. Saito, Nazalan Najimudin and Maqsudul Alam. (2014)Crystal structure of truncated hemoglobin from extremely thermophilic and acidophilicbacterium. Journal of Biochemistry 156: 97-106.
Farrukh Jamil, Naeem Rashid, Qurra-tul-Ann Afza Gardner and Muhammad Akhtar. (2011) Gene cloning and characterization of glycine oxidase from Bacillus subtilis strain R5. Biologia 66: 1-7.
Farrukh Jamil, Qurra-tul-Ann Afza Gardner, Qamar Bashir, Naeem Rashid and Muhammad Akhtar. (2010) Mechanistic and stereochemical studies of glycine oxidase from Bacillus subtilis strain R5. Biochemistry 49: 7377-7383.
Bowyer , H. Mikolajek , J.W. Stuart , S.P. Wood, F. Jamil , N. Rashid , M. Akhtar and J.B. Cooper. (2009) Structure and Function of the L-threonine dehydrogenase (Tk-TDH) from the hyperthermophilic archaeon Thermococcus kodakaraensis. Journal of Structural Biology 168:294-304.
Qamar Bashir, Naeem Rashid, Farrukh Jamil, Tadayuki Imanaka and Muhammad Akhtar. (2009)Highly thermostable L-threonine dehydrogenase from the Hyperthermophilic Archaeon Thermococcus Kodakaraensis.Journal of Biochemistry 146:95-102.
Mehnaz, Samina, Deeba Noreen Baig, Farrukh Jamil, Brian Weselowski and George Lazarovits. (2009) Characterization of a phenazine and hexanoyl homoserine, lactone producing pseudomonoas aurantiaca strain PB-st2, isolated from sugarcane stem. J. Microbiol. Biotechnol. 19:1688-1694.
Bowyer,H. Mikolajek, J. N. Wright, A. Coker,P. T. Erskine, J. B. Cooper, Q. Bashir and N. Rashid, F. Jamil and M. Akhtar. (2008) Crystallization and preliminary X-ray diffraction analysis of L-threonine dehydrogenase (TDH) from the hyperthermophilic archaeon Thermococcus Kodakaraenisis. Acta crystallographica Section F. 64:828-30.
Neenish Rana, Nosheen Ehsan, Awais Ihsan, Farrukh Jamil (First revision submitted) In Silico analyses of the pseudogenes of Helicobacter Pylori. Pak. Jour of Zoology.
Structural studies of thermophilic and acidophilic bacterial hemoglobins under the APEX grant to Centre for Chemical Biology, University Sains Malysia.
Mechanistic and stereochemical studies of 2-amino-3-ketobutyrate CoA ligase under the HEC indigenous PhD grant.
Production of biodegradable plastic from rapeseed press cake. 0.5 M grant by HEC.
BS Hons THESIS GUIDED: 9 in the field of Biochemistry, Bioinformatics and Microbiology.
M. Phil/MS THESIS GUIDED: 5 Working: 3
Production of biodegradable plastic from rape seed press cake
Genetic susceptibility variants of FGFR2, MAP3K1, and TNRC9 for Breast Cancer in female patients of Pakistan
Identification and molecular characteristics of different bacteria from raw milk
Studies on Side Effects of antituberculosis Therapy in District
Association studies of common variants of TCF4 gene conferring risk of Schizophrenia in Pakistani individuals
Small ligand docking in novel truncated hemoglobins
Mutational screening for sodium channel, voltage gated, type I for Epilepsy patients in Sahiwal
WORKSHOPS AND CONFERENCES
Participated in a workshop on “Radiation Safety” held in the Universiti Sains, Malaysia, 2012.
Participated in a workshop on “Applications of Schrodinger software in molecular dynamic simulations” held in the Centre for Chemical Biology, Universiti Sains Malaysia, July 2011.
Participation as an active member in symposium on “Working with proteins in Post-Genomic Era” held in the School of Biological Sciences, Pakistan, on January 2010.
Participated in an international workshop on “Separation Science and the Omics” held in the School of Biological Sciences during April, 2009 and conducted a laboratory session on protein purification by using FPLC.
Participation as a member of organising committee of 18th Federation of Asian and Oceanian Biochemists and Molecular Biologists (FAOBMB) Symposium held at Lahore, Pakistan on November, 2005.
Three days training session on “Nimblegen Gene Expression Microarray” organised in the Centre for Chemical Biology by Roche, September 2013.
Three days training on MALDI-TOF organised in Centre for herbal standardisation, Malaysia, May 2013.
Five days training on Pathway Studio in the Centre for Chemical Biology, July 2012.
HONOURS AND AWARDS
Postdoctoral fellowship by Universiti Sains Malaysia (Dec 2010- Dec 2013)
PhD scholarship by Higher Education Commission of Pakistan (2005-2010)