Edlund et al. – American Journal of Botany 2016. DOI: 10.3732/ajb.1600031 – Appendix S1 – Page 1
Appendix S1. Plants surveyed for pollen germination site. When collected at botanical gardens, stigmas were cross pollinated, if possible, using a paintbrush, and the entire carpel was collected into 50% Karnovsky’s fixative; pollen was also dusted onto solidified pollen growth medium. From other field sites, entire plants were transferred to the laboratory (in a cooler with an ice pack), before pollen was dusted in situ or in vitro; plants were then pressed for voucher specimens.