Performance Report for Cooperative Agreement No: na06oar4810163 for the Period from September 1, 2006 to August 31, 2012 University of Maryland Eastern Shore



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LMRCSC Cruise Evaluation: An evaluation form aimed at assessing the students’ experience aboard LMRCSC research cruises was created and distributed to participants in the annual research cruise. More than 10 completed surveys were received. General comments from the evaluations received include: 1. “I find research cruises to be an excellent source of experience, and provide more hands on learning.” 2.“Yes I would [participate again], but preferably in the summer”.

Evaluation Forms for interns and mentors: Thirty-seven (37) students provided evaluations of their internship experiences at various NOAA facilities, LMRCSC partners and other institutions. Similarly, 17 supervisors provided evaluations of the LMRCSC students who participated in internships in their labs. The data provided were utilized by the Center to ensure the best possible internship experiences for both students and their supervisors.
Goal 2: Enhance Research Programs in the Marine Sciences at Participating Institutions

Objective 1: Continue Current and Develop New Research Programs Congruent with NOAA Fisheries Research Priorities

LMRCSC scientists and students worked on projects in four thematic areas: Quantitative Fisheries, Essential Fish Habitat, Fisheries Socio-economics and Aquaculture. Some of these projects were funded through the TAB review process, while others were supported by external grant funds. TAB projects included NOAA scientists as collaborators, and students as active participants.

Xaymara Serrano completed her M.S. thesis at RSMAS on the physiological definition of habitat constraints for grey snapper in South Florida in June 2008, and has been working on a Ph.D. project with Dr. Andrew Baker on impacts of climate change on coral reef communities. Her dissertation is entitled “Population Genetics of Scleractinian Corals in the Tropical Western Atlantic (Caribbean). Joel Llopiz who completed his Ph.D. also at RSMAS in June 2008 worked on population dynamics parameters during the early life history of billfish. Research in Socio-economics was conducted by Ayeisha Brinson (Ph.D., RSMAS) on the Atlantic billfish fisheries in collaboration with Dr. David Die (RSMAS) and Dr. Juan Agar (NMFS/SEFSC). Dwight Ebanks continued his work on the effects of climate change and ocean acidification on early life history of cobia, a project that has applications to both aquaculture and essential fish habitat. Quantitative fisheries is the main area of research of the LMRCSC Project Director at RSMAS whose work contributed to the assessment of International Commission for the Conservation of Atlantic Tunas (ICCAT) and billfish species. Dr. Die also provided support to the Center for Independent Experts that provides NMFS with independent peer review of science products. Additionally, he was a leader of the pelagic working group during the Caribbean Fisheries Mechanism annual assessment workshop.

Two LMRCSC institutions, UMES and HU collaborated with 7 other institutions and submitted a proposal to NOAA entitled “Cooperative Institute for Climate and Marine Ecosystem Research” with the University of Rhode Island as the lead institution, although the proposal was not funded. UMES also collaborated with, Morgan State University, and Virginia Institute of Marine Sciences in a proposal submitted to NSF to establish a Center for the integrated study of coastal ecosystem processes and dynamics in the mid-Atlantic region which was funded in 2010.

Dr. Matt Ogburn (SSU) developed a shrimp project with Dr. Joan Browder and other NOAA scientists at NOAA SEFSC. Existing NOAA collaborations with NOAA’s Gray’s Reef National Marine Sanctuary (GRNMS) continued during this project period through projects such as that of M.S. student, Noelle Hawthorne (SSU) that involved monitoring of the utilization of the reef by commercially important species.

The LMRCSC at SSU made a greater effort to include fishery socioeconomics in its research activities by engaging Dr. Kristy Wallmo (NOAA, Silver Spring), Patricia Pinto da Silva (NOAA Woods Hole Lab), and Dr. Susan Abbot-Jamison (NOAA, Silver Spring) of the Voices of the Fisheries Project. The three collaborated on a project to study the history of African-American fishermen in coastal Georgia that was funded by the NOAA Preserve America Program.

Scientists at the LMRCSC worked with Elizabeth City State University scientists to develop projects looking at the evolution of coupled human and natural systems. A symposium entitled “Social and Economic Dimensions of Ecosystem Based Fisheries Management” was planned by Dr. Eric May (UMES), Dr. Anne Garland (ECSU) and Dr. Kristy Wallmo (NOAA Office of Science and Technology) for the AFS annual meeting in August, 2008.

The research projects funded through the TAB review process of the LMRCSC are presented in Tables 9-13. A total of fifty-four (54) proposals were funded from 2006 to 2012. The projects were collaborative and included scientists from center institutions, NOAA and students.


Table 9. LMRCSC Projects Funded After Technical Advisory Board Proposal Review (2006-2007).

2006-2007 LMRCSC Approved Projects

Investigators

Project Title

Amount of Award

Carla Curran (SSU)

Paul Pennington (NOAA)




Effect of isopod parasite Probopyrus pandalicola on the grass shrimp Palaeomonetes pugio: Impacts on population density, gravidity, and tolerance to contaminants

$31,417.00



Dewayne Fox (DSU)

Brad Wetherbee (URI)

Nancy Kohler (NOAA)

Camilla McCandless (NOAA)



Habitat use, depth selection & timing of residency for sand tiger sharks (Carcharias taurus) in DE Bay



$40,384.00


Ali Ishaque (UMES)

Yan Waguespack (UMES)

Chris Chambers (NOAA)


Evaluation of Interactive effects of contaminants on full life cycle responses for assessment of habitat quality


$45,007.00



Andrea Johnson (UMES)

Anne Richards (NOAA)



Biology of large monkfish, Lophius americanus


$40,950.00


Joseph Love (UMES)

Gretchen Martin (NOAA)



Seasonal differences in larval Ingression through Ocean City Inlet (Maryland)

$21,739.00


Refik Orhun (RSMAS)

Daniel Benetti (RSMAS)

Rosemary Jagus (COMB)


Evaluation of adaptation to stress conditions in cobia larvae and juveniles cultured with and without the use of probiotics

$41,667.00

Eric Schott (COMB)

Rosemary Jagus (COMB)

Joseph Pitula (UMES)

Dionne Hoskins (SSU)



Development of strain and species-specific probes to investigate reservoirs and genetic diversity of the blue crab parasite Hematodinium spp.


$49,748.00


Yossi Tal (COMB)

Yonathan Zohar (COMB)

Daniel Benetti (RSMAS)

Rosemary Jagus (COMB)



Evaluation of cobia (Rachycentron canadum) production in a fully contained, environmentally sustainable recirculating aquaculture system.


$48,728.00


John Trant (COMB)

Andrea Johnson (UMES)



Evaluation of chicken Litter as a Feminizing Agent in Striped Bass

$21,466.00


Yan Waguespack (UMES)

Andrew Draxler (NOAA)

Ashok Deshpande (NOAA)


Experimental assessment of the effects of contaminated sediments from the Lower Hudson-Raritan estuary and Elizabeth River on Winter Flounder

$45,200.00



Eric Wooden (HU)

Kenneth Moore (NOAA)



Water quality monitoring in the Hampton River


$23,075.00


Table 10. LMRCSC Projects Funded After Technical Advisory Board Proposal Review (2007-2008).


Investigators

Project Title

Amount of Award

Reginald Black (UMES), Ryan Corbin (UMES)

Joseph Love (UMES)

Howard Townsend (NOAA COL)


Recruitment and diets of fishes in coastal lagoons of Maryland

$13,203.00

Carla Curran (SSU)

Paul Pennington (NOAA)



The Effect of the Isopod Parasite Probopyrus pandalicola on the Grass Shrimp Palaemonetes pugio: Impact on population density, gravidity, and tolerance to contaminants

$35,872.00

Dewayne Fox (DSU)

Cami McCandless (NOAA)

Nancy Kohler (NOAA)

Brad Wetherbee (URI)



Habitat use, depth selection, and the timing of residency for sand tiger shark (Carcharias taurus) in Delaware Bay

$36,691.00

Dewayne Fox (DSU)

Kyle Shertzer (NOAA)

Larissa Bailey (USGS)


Estimation of survival, emigration, and fishing mortality rates for American eel through a combined use of acoustic telemetry and mark-recapture

$39,978.00

William Gardner (UMES)

Eric May (UMES)

Ashok Deshpande (NOAA)


Analysis of PCB Congener Accumulation in Striped Bass (Morone saxatilis) in the Chesapeake Bay and Atlantic Coastal Waters

$31,900.00

Lonnie Gonsalves (UMES)

Eric May (UMES)

Rosemary Jagus (COMB)

Ashok Deshpande (NOAA)

John Jacobs (NOAA)


Essential Fatty Acid Composition and Immune-competence of Chesapeake Bay Striped Bass Morone saxatilis

$35,238.00

Ali Ishaque (UMES)

Yan Waguespack (UMES)

Chris Chambers (NOAA)


Evaluation of interactive effects of contaminants on full life-cycle responses for assessing fish habitat quality in estuaries

$46,059.00

Andrea Johnson (UMES)

Anne Richards (NOAA)



Effects of temperature change on the distribution and behavior in Lophius americanus

$44,215.00

Daniel Luers (UMES)

Joseph Love (UMES)

Gretchen Bath Martin (NOAA)

Ken Able (Rutgers)



Mentoring Undergraduate, Minority Students in Coastal Ecology of Larval Fishes.

$10,247.00

Josh Newhard (UMES)

Joseph Love (UMES)

John Gill (USFWS)


Growth of white perch (Morone americana) from an internationally recognized estuary of Chesapeake Bay

$37,321.00

Joseph Pitula (UMES

Feng Chen (COMB)

Moti Harel (Advanced Bio-Nutrition)


Impact of the Probiotic Dietary Supplement Lactobacillus acidophilus on Rainbow Trout

$23,793.00

Eric Schott (COMB)

Joseph Pitula (UMES)

Dennis McIntosh (DSU)

John Jacobs (NOAA)



Validation of molecular probes for use in investigating environmental reservoirs of crab disease

$40,795.00

Don Bacoat (RSMAS)

Refik Orhun (NOAA)

Dan Benetti (RSMAS)

Rosemary Jagus (COMB)



Evaluation of adaptation to stress conditions in cobia larvae cultured with and without the use of probiotics

$4,795.00


Table 11. LMRCSC Projects Funded After Technical Advisory Board Proposal Review (2008-2009).

Investigators

Project Title

Amount of Award

Andrea Johnson (UMES)

Anne Richards (NEFSC)

Daniel Cullen (M.S. student UMES)


Effects of temperature change on the distribution and behavior in Lophius americanus (year 2)

$34,192.00

Lonnie Gonsalves (PhD student UMES)

Rosemary Jagus (UMBI-COMB)

Eric May (UMES)

Ashok Deshpande (NEFSC)

John Jacobs (NOAA COL)


Essential fatty acid composition and immune-competence of Chesapeake Bay Striped Bass Morone saxatilis

$37,536.00

Eric Schott (UMBI-COMB)

Joseph Pitula (UMES)

Gretchen Messick (NOAA COL)

Dennis McIntosh (DSU)



Investigation into persistence and distribution of sediment reservoirs of Hematodinium sp., a protistan parasite of blue crab

$50,711.00

Dan Terlizzi (UMBI-COMB)

Dennis McIntosh (DSU)

Gary Wikfors (NEFSC)

Francis Chrest (UMBI-COMB)



Programmed cell death in Karlodinium micrum, it's induction by environmental factors and potential in bloom management

$36,132.00

Robert Cowen (RSMAS)

Gabriel Tsechpenakis (Univ of Miami)

Jon Hare (NEFSC)

Victor Restropo (SEFSC)



Quantification of fishery resources: application of new imaging and computer vision technology

$58,800.00

Joseph Love (UMES)

Gretchen Bath Martin (SEFSC)

Mike Luisi (MDDNR)


Role of larval ingress and estuarine conditions on recruitment for Atlantic croaker, spot, and summer flounder in Maryland's coastal lagoons

$6,445.00

Harold Schreier (UMBI-COMB)

Eric Schott (UMBI-COMB)

Dennis McIntosh (DSU)

Gary Wikfors (NEFSC)

Diane Kapareiko (NEFSC)


Role of probiotic bacteria in protecting oyster larvae from pathogenic bacteria in hatchery culture

$35,966.00

Carla Curran (SSU)

Paul Pennington (SEFSC)

Krystle Ludwig (M.S. student SSU)


The effects of coded wire tags on the growth of the grass shrimp Palaemonetes pugio in mesocosms

$47,434.00

Andrea Johnson (UMES)

Anne Richards (NEFSC)

Joel Carlin (Gustavus Adolphus College)


The use of microsatellite DNA to evaluate US fishery management areas and effective population size of monkfish, Lophius americanus

$18,356.00


Table 12. LMRCSC Projects Funded After Technical Advisory Board Proposal Review (2009-2010).

Project Title

Investigators

Amount of Award

Joseph Pitula (UMES), Eric May (UMES), Feng Chen (COMB), Eric Schott (COMB), Frank Morado (NOAA), Carl Zimmerman (NPS)

Correlation of biotic and abiotic factors in environmental presence of Hematodinium

$36,536.00

Carla Curran (SSU), Paul Pennington (NOAA)


Effect of coded wire tags and Probopyrus pandalicola on the susceptibility of grass shrimp to predation in mesocosms-Year 2

$48,898.00

Dewayne Fox (DSU), Larissa Bailey (USGS)

Paul Conn (NOAA), Kyle Shertzer (NOAA)




Estimation of survival, emigration and fishing mortality rates for American eel through telemetry and mark-recapture-Year 2

$39,393.00

Dwight Ebanks (RSMAS), Dan Benetti (RSMAS)

Nelson Ehrhardt (RSMAS), Rose Jagus (COMB)



Influence of predicted aqueous hypercapnia on cobia prefertilization and larval stages

$27,351.00

J. Sook Chung (COMB), Joseph Pitula (UMES)

Gretchen Messick (NOAA)



Measuring environmental and physiological stress and its impact on infection in blue crab by Hematodinium

$35,835.00

Eric Schott (COMB), Matthew Ogburn (SSU),

Susan White (NOAA)



Molecular tracking of pathogens in early life history of blue crabs

$40,454.00

Andrea Johnson (UMES), Yonathan Zohar (COMB)

Richard Brill (NOAA)



Reproductive and immunological health assessment of Atlantic croaker exposed to hypoxia in Chesapeake Bay

$24,880.00

Andrij Horodysky (HU), Andrea Johnson (UMES)

Richard Brill (NOAA)



Sensory ecology of juvenile and adult black sea bass

$33,159.00

Harold Schreier (COMB), Eric Schott (COMB)

Dennis McIntosh (DSU), Gary Wikfors (NOAA)



Understanding the interaction of probiotic and pathogenic bacteria in oyster larvae culture

$39,715.00

Andrea Johnson (UMES), Allen Place (COMB)

Anne Richards (NOAA)



Use of DNA markers to evaluate US fishery management areas and effective pop. size of monkfish

$23,174.00


Table 13. LMRCSC Projects Funded After Technical Advisory Board Proposal Review (2010-2011).

Investigators

Project Title

Amount of Award

1. Joe Pitula (UMES), Feng Chen (IMET), Eric May (UMES), Courtney Schupp (NPS)

Correlation of biotic and abiotic factors in environmental presence of Hematodinium

$28,842.00

2. Sook Chung (IMET), Joe Pitula (UMES), Gretchen Messick (NOAA)

Determining critical stages of Hematodinium infection and measuring environmental and physiological stress impact on infection

$31,179.00

3. Brad Stevens (UMES), Beth Babcock (RSMAS), Gary Shepard (NOAA)

Development of in-situ assessment and observation methods for black sea bass

$13,420.00

4. Adam Tulu (UMES-Student), Ali Ishaque (UMES), Rosemary Jagus (IMET), Chris Chambers (NOAA)

Development of molecular tools and methodologies to evaluate the effect of marine pollutants on Atlantic tomcod, Microgadus tomcod

$27,002.00

5. Matt Ogburn (SSU), Dionne Hoskins (SSU), Howard Schnabolk (NOAA)

Evaluation of restored intertidal oyster reefs using lidar

$22,060.00

6. Dionne Hoskins (SSU), Susan Abbott-Jamison (NOAA), Michael Jepson (NOAA)

Piecing together the historical role of African-Americans in Georgia's coastal fisheries using oral histories

$7,600.00

7. Andrij Horodysky (HU), Andrea Johnson (HU), Richard Brill (NOAA)

Sensory ecology of tautog: ecophysiological auditory and visual performance measures

$21,108.00

8. Al Place (IMET), Tom Rippen (UMES), James Morris (NOAA)

Taurine – the missing ingredient for development of fish free diets for aquaculture?

$34,297.00

9. Andrea Johnson (UMES), Andrij Horodysky (HU), Richard Brill (NOAA)

Temperature preferences of Atlantic croaker under hypoxic and normoxic conditions

$41,614.00

10. Carla Curran (SSU), Paul Pennington (NOAA)

The effect of structure on predation rates of parasitized and unparasitized grass shrimp

$30,500.00

11. Eric Schott (IMET), Dennis McIntosh (DSU), Gretchen Messick (NOAA)

Tracking pathogens of blue crabs along a climatological and latitudinal gradient

$41,217.00

12. Belita Nguluwe (UMES-Student), Andrea Johnson (UMES), Al Place (IMET), Anne Richards (NOAA)

Use of mitochondrial DNA markers to evaluate US Fishery management areas and effective population size of monkfish

$9,000.00


Examples of LMRCSC Collaborative Projects Funded After TAB Reviews of Proposals Submitted to the Center (2006 – 2012)
Summaries of examples of the projects that highlight results of the TAB funded research and their significance to NOAA Fisheries and LMRCSC are provided below.

1. Development of in-situ assessment and observation methods for black sea bass, Centropristis striata. (Appendix IIe, 2010-2011). Investigators: Dr. Bradley Stevens (UMES) and Dr. Beth Babcock (RSMAS) NOAA Collaborator(s): Gary Shepherd, NOAA, NEFSC, Woods Hole, MA.; Vincent Guida, NEFSC, J.J. Howard Research Laboratory, Sandy Hook, NJ; LMRCSC Student(s): Dan Cullen (Ph.D. Student, UMES); Courtney McGeachy (MS Student, UMES). Figure: Black sea bass,

http://www.safmc.net/fishidandregs/fishgallery/blackseabass.

Black sea bass (Centropristis striata) support important commercial and recreational fisheries in the Mid- Atlantic Bight, but live in rugose habitats that cannot be sampled by NOAA trawl surveys, so there is no acceptable index of abundance for them. Over two summers, methods were developed for estimating abundance of black sea bass using in-situ video technology, and observing fish behavior towards traps. Cameras were attached to either modified commercial fish traps or to stand-alone frames, and observations made over several hours at 6 sites of varying habitat rugosity at distances of 12-20 miles off the Maryland coast. Results showed that video counts can be obtained with reasonable precision, that fish counts are related to substrate type, and that video-based counts are correlated with quantitative fish catch estimates using hook-and-line. Behavioral observations revealed that only a small proportion (1-3%) of fish that approach traps enter and stay in the trap. Plans for research in FY13 include assessing the effects of habitat, depth, and other variables on video fish counts. NOAA is required to assess the abundance of fish stocks, but has very little usable data for black sea bass. This project will provide protocols for producing video-based indices of fish abundance that can be incorporated into the data base of information available for stock assessment. This project has formed the basis of thesis research for two graduate students, Courtney McGeachey (MS, 2012), and Dan Cullen (PhD, 2014). Numerous other graduate and undergraduate students have assisted in the project and learned about in-situ technology, and the data and approaches used have been integrated into Dr. Stevens class “Survey Sampling”, which is taught as part of the LMRCSC and Professional Science Master’s degree Program in quantitative fisheries and resource economics.

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