Appendix 2-5: Rejected ecotox bibliography Excluded
acetylcholinesterase (AChE) purified from rat brain
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- Two OP compounds, malathion and chlorpyrifos could be detected in the range of 0.1-100 nM and 0.1-70 nM, respectively at 2.0-3.0% inhibition level of AChE. T
| acetylcholinesterase (AChE) purified from rat brain was immobilized onto gold nanoparticles (AuNPs) assembled on the surface of porous calcium carbonate (CaCO(3)) microsphere. The resulting AChE-AuNPs-CaCO(3) bioconjugate was mounted on the surface of Au electrode with the help of silica sol-gel matrix to prepare the working electrode. This electrode was connected to Ag/AgCl (3 M/saturated KCl) as standard and Pt wire as an auxiliary electrode through a potentiostat to construct an organophosphorus (OP) biosensor. The biosensor was based on inhibition of AChE by OP compounds/insecticides. The biosensor showed optimum response at pH 7.0, 30 degrees C, when polarized at +0.2 V. Two OP compounds, malathion and chlorpyrifos could be detected in the range of 0.1-100 nM and 0.1-70 nM, respectively at 2.0-3.0% inhibition level of AChE. The sensor was reactivated by immersing it in 0.1 mM 2-pyridine aldoxime for 10 min. The detection limit of the sensor was 0.1 nM for both malathion and chlorpyrifos. The biosensor exhibited good reusability (50 times without considerable loss) and storage stability (50% within 60 days, when stored at 4 degrees C). (C) 2011 Elsevier B.V. All rights reserved.
Number of Volumes: 5 ISI Document Delivery No.: 846XT 134. Chauhan, Nidhi; Narang, Jagriti; Pundir, C S, and Chauhan, Nidhi. Immobilization of Rat Brain Acetylcholinesterase on Zns and Poly(Indole-5-Carboxylic Acid) Modified Au Electrode for Detection of Organophosphorus Insecticides. 2011 Nov 15; 29, (1): 82-88. Rec #: 3030 Keywords: IN VITRO Notes: Chemical of Concern: MLN Abstract: Abstract: A novel, highly sensitive amperometric biosensor for detection of organophosphorus (OP) compounds has been constructed, based on rat brain acetylcholinesterase (AChE) immobilized onto nanocomposite of ZnS-nanoparticles (ZnSNPs) and poly(indole-5-carboxylic acid) electrodeposited on Au electrode. In the presence of acetylthiocholine chloride (ATCl) as a substrate, ZnSNPs promoted electron transfer reactions at a lower potential and catalyzed electrochemical oxidation of enzymatically formed thiocholine, thus increasing detection sensitivity. Under optimum conditions (phosphate buffer, pH 7.5 and 30 degree C), the inhibition of AChE by malathion and chlorpyrifos was proportional to their concentrations in the range, 0.1-50nM and 1.5-40nM, respectively. The biosensor determined malathion and chlorpyrifos in spiked tap water samples with a acceptable accuracy (95-100%). The enzyme electrode had long-storage stability (50% retention of initial activity within 2months, when stored at 4 degree C). Keywords: Acetylcholinesterase Keywords: Brain Keywords: Enzymes Keywords: Chloride Keywords: Electron transfer Keywords: CSA Neurosciences Abstracts; Biotechnology and Bioengineering Abstracts Keywords: Malathion Keywords: N3 11145:Methodology Keywords: Biosensors Keywords: Chlorpyrifos Keywords: Insecticides Keywords: Phosphate Keywords: Electrodes Keywords: Oxidation Keywords: W 30955:Biosensors Keywords: pH effects Keywords: Immobilization English. Date revised - 2011-11-01. Last updated - 2012-08-10. DOI - 53941068-f065-4a3c-a199csamfg201; 15763427; 0956-5663. SubjectsTermNotLitGenreText - Acetylcholinesterase; Brain; Enzymes; Chloride; Electron transfer; Malathion; Biosensors; Chlorpyrifos; Insecticides; Phosphate; Oxidation; Electrodes; pH effects; Immobilization 135. Chauhan, Nidhi; Pundir, Chandra Shekhar, and Chauhan, Nidhi. An Amperometric Biosensor Based on Acetylcholinesterase Immobilized Onto Iron Oxide Nanoparticles/Multi-Walled Carbon Nanotubes Modified Gold Electrode for Measurement of Organophosphorus Insecticides. 2011 Sep 2; 701, (1): 66-74. Rec #: 3170 Keywords: CHEM METHODS Notes: Chemical of Concern: MLN Abstract: Abstract: An acetylcholinesterase (AChE) purified from maize seedlings was immobilized covalently onto iron oxide nanoparticles (Fe sub(3O) sub(4)NP) and carboxylated multi walled carbon nanotubes (c-MWCNT) modified Au electrode. An organophosphorus (OP) biosensor was fabricated using this AChE/Fe sub(3O) sub(4)/c-MWCNT/Au electrode as a working electrode, Ag/AgCl as standard and Pt wire as an auxiliary electrode connected through a potentiostat. The biosensor was based on inhibition of AChE by OP compounds/insecticides. The properties of nanoparticles modified electrodes were studied by scanning electron microscopy (SEM), Fourier transform infrared (FTIR), cyclic voltammograms (CVs) and electrochemical impedance spectroscopy (EIS). The synergistic action of Fe sub(3O) sub(4)NP and c-MWCNT showed excellent electrocatalytic activity at low potential (+0.4 V). The optimum working conditions for the sensor were pH 7.5, 35 degree C, 600 mu M substrate concentration and 10 min for inhibition by pesticide. Under optimum conditions, the inhibition rates of OP pesticides were proportional to their concentrations in the range of 0.1-40 nM, 0.1-50 nM, 1-50 nM and 10-100 nM for malathion, chlorpyrifos, monocrotophos and endosulfan respectively. The detection limits were 0.1 nM for malathion and chlorpyrifos, 1 nM for monocrotophos and 10 nM for endosulfan. The biosensor exhibited good sensitivity (0.475 mA mu M super(-1), reusability (more than 50 times) and stability (2 months). The sensor was suitable for trace detection of OP pesticide residues in milk and water.) Keywords: Sensors Keywords: iron oxides Keywords: Pesticide residues Keywords: Acetylcholinesterase Keywords: ENA 09:Land Use & Planning Keywords: monocrotophos Keywords: Spectroscopy Keywords: Malathion Keywords: Biosensors Keywords: Insecticides Keywords: Carbon Keywords: Zea mays Keywords: Biotechnology and Bioengineering Abstracts; Environment Abstracts Keywords: W 30955:Biosensors Keywords: Gold Keywords: pH effects Keywords: Milk Keywords: Endosulfan Keywords: Chlorpyrifos Keywords: Electrodes Keywords: Pesticides Keywords: nanotubes Keywords: Seedlings Keywords: Iron Keywords: nanoparticles Keywords: nanotechnology English. Date revised - 2012-01-01. Last updated - 2012-09-10. DOI - 46479967-2ac4-461a-852bcsaobj201; 15372226; 0003-2670. SubjectsTermNotLitGenreText - Milk; iron oxides; Pesticide residues; Acetylcholinesterase; monocrotophos; Spectroscopy; Malathion; Endosulfan; Biosensors; Chlorpyrifos; Carbon; Insecticides; Electrodes; Pesticides; Gold; nanotubes; Seedlings; pH effects; nanoparticles; Sensors; Iron; nanotechnology; Zea mays 136. Chauzat, M. P. and Faucon, J. P. Pesticide Residues in Beeswax Samples Collected from Honey Bee Colonies (Apis mellifera L.) in France. 2007; 63, 1100-1106. Rec #: 1670 Keywords: SURVEY Call Number: NO SURVEY (AZ,CMPH,CPY,CYF,CYP,DM,ES,FNT,MDT,MLN,MP,MVP,TAUF,VCZ) Notes: Chemical of Concern: AZ,CMPH,CPY,CYF,CYP,DM,EPRN,ES,FNT,FNTH,HCCH,MDT,MLN,MP,MVP,PPCP,PRN,TAUF,VCZ 137. Cheesman, Matthew J.; Traylor, Matthew J.; Hilton, Margaret E.; Richards, Katelyn E.; Taylor, Matthew C.; Daborn, Phillip J.; Russell, Robyn J.; Gillam, Elizabeth M. J., and Oakeshott, John G. Soluble and membrane-bound Drosophila melanogaster CYP6G1 expressed in Escherichia coli: Purification, activity, and binding properties toward multiple pesticides. 2013 May; 43, (5): 455-465. Rec #: 1740 Keywords: IN VITRO Notes: Chemical of Concern: MLN Abstract: Cytochrome P450 CYP6G1 has been implicated in the resistance of Drosophila melanogaster to numerous pesticides. While in-ávivo and in-ávitro studies have provided insight to the diverse functions of this enzyme, direct studies on the isolated CYP6G1 enzyme have not been possible due to the need for a source of recombinant enzyme. In the current study, the Cyp6g1 gene was isolated from D.-ámelanogaster and re-engineered for heterologous expression in Escherichia coli. Approximately 460-ánmol-áLęĆ1 of P450 holoenzyme were obtained in 500-ámL cultures. The recombinant enzyme was located predominantly within the bacterial cytosol. A two-step purification protocol using Ni-chelate affinity chromatography followed by removal of detergent on a hydroxyapatite column produced essentially homogenous enzyme from both soluble and membrane fractions. Recombinant CYP6G1 exhibited p-nitroanisole O-dealkylation activity but was not active against eleven other typical P450 marker substrates. Substrate-induced binding spectra and IC50 values for inhibition of p-nitroanisole O-dealkylation were obtained for a wide selection of pesticides, namely DDT, imidacloprid, chlorfenvinphos, malathion, endosulfan, dieldrin, dicyclanil, lufenuron and carbaryl, supporting previous in-ávivo and in-ávitro studies on Drosophila that have suggested that the enzyme is involved in multi-pesticide resistance in insects. Cytochrome P450/ CYP6G1/ Pesticide/ Multi-pesticide resistance/ Insecticide resistance http://www.sciencedirect.com/science/article/pii/S0965174813000271 138. Chen, D.; Chen, C., and Du, D. Detection of Organophosphate Pesticide Using Polyaniline and Carbon Nanotubes Composite Based on Acetylcholinesterase Inhibition. Rec #: 9370 Keywords: CHEM METHODS Notes: Chemical of Concern: MLN Abstract: ABSTRACT: Acetylcholinesterase (AChE) activity may be useful biomarker for detecting of organophosphate pesticides (OP). Thus a sensitive biosensor for quantitative determination of OP based on AChE biomonitoring was developed. Multi-walled carbon nanotube (MWCNT)/polyaniline (PANI) composite film was prepared by electrochemical polymerization. The immobilized AChE catalyzed the hydrolysis of acetylthiocholine chloride to produce thiocholine, which engendered an irreversible oxidation peak. The enzyme activity was monitored by measuring the oxidation current of thiocholine and further detection of OP. The developed sensor provided a new promising tool for pesticide analysis and assay of enzyme activity. MESH HEADINGS: Acetylcholinesterase MESH HEADINGS: *Aniline Compounds/chemistry MESH HEADINGS: Biosensing Techniques/methods MESH HEADINGS: Cholinesterase Inhibitors/analysis MESH HEADINGS: Electrochemistry MESH HEADINGS: Enzymes, Immobilized MESH HEADINGS: Malathion/analysis MESH HEADINGS: *Nanocomposites/chemistry MESH HEADINGS: Nanotechnology MESH HEADINGS: *Nanotubes, Carbon MESH HEADINGS: Organophosphorus Compounds/*analysis MESH HEADINGS: Pesticides/*analysis eng 139. Chen, D.; Song, Z., and Lv, H. Assay of Picogram Level Isocarbophos Residue on Tangerines and Oranges With Luminol-Albumin Chemiluminescence System. Rec #: 8370 Keywords: CHEM METHODS Notes: Chemical of Concern: MLN Abstract: ABSTRACT: A sensitive flow injection-chemiluminescence (FI-CL) method for the determination of isocarbophos (ICP) residue on tangerines and oranges was proposed. It was found that the CL intensity from luminol-albumin CL reaction could be obviously quenched in the presence of ICP and the decrease in CL intensity was proportional to the logarithm of ICP concentrations ranging from 1.0 to 1000 pmol L(-1), giving the limit of detection of 0.3 pmol L(-1) (3σ). The proposed procedure was successfully applied to the determination of ICP residue on tangerines and oranges with recoveries varying from 92.0 to 111.0% and RSDs less than 5.0%. The possible CL mechanism of luminol-albumin-ICP reaction was discussed, and ICP to albumin's binding constant (K(D)=1.00 × 10(6) L mol(-1)) and the number of binding sites (n=1.00) were given by the homemade FI-CL model. MESH HEADINGS: Albumins/chemistry MESH HEADINGS: Citrus/*chemistry MESH HEADINGS: Citrus sinensis/*chemistry MESH HEADINGS: Insecticides/*analysis MESH HEADINGS: Limit of Detection MESH HEADINGS: Luminescent Measurements/instrumentation/*methods MESH HEADINGS: Luminol/chemistry MESH HEADINGS: Malathion/*analogs & MESH HEADINGS: derivatives/analysis MESH HEADINGS: Pesticide Residues/*analysis eng 140. Chen, Jing-Min and Chen, Jing-Min. Determination of Malathion in Drinking Water by Rapid Solvent Extraction-Gas Chromatography. 2011 Sep; 28, (9): 814-815. Rec #: 3200 Keywords: CHEM METHODS Notes: Chemical of Concern: MLN Abstract: Abstract: Objective: To establish a method for the determination of malathion in drinking water by rapid solvent extraction-gas chromatography. Methods: The sample volume was 25 ml, static extraction for 2 times, extraction time was 6 min, the solvent was methylene chloride-petroleum ether-acetone (volume ratio of 3:1:1), the mass of anhydrous sodium sulfate was 20 g. HP-5 capillary column(30 m x 0.32 mm, 0.25 mu m)was used, split ratio was 1:5, gas chromatography was used for the determination. Results: A good linear relationship was seen in the concentration of 1.0-500.0 mu g/L, the regression equation was gamma =0.1146x + 0.0153, r value was 0.9996, the lowest detection limit was 0.05 mu g/L, the quantitative referrals was 1.04 mu g/L. the rate of recovery ranged from 98.3% to 100.9%. RSDs was 0.36% to 4.23%. Conclusion: This method is simple, accurate, precise and applicable to the determination of malathion in drinking water. Keywords: AQ 00001:Water Resources and Supplies Keywords: Sulfates Keywords: Mathematical models Keywords: Chromatographic techniques Keywords: Chromatography Keywords: SW 0810:General Keywords: Solvents Keywords: Q5 01502:Methods and instruments Keywords: Water Resources Abstracts; ASFA 3: Aquatic Pollution & Environmental Quality; Aqualine Abstracts Keywords: Gas Chromatography Keywords: Malathion Keywords: Sodium Keywords: Drinking Water Keywords: Analytical Methods Keywords: Detection Limits Keywords: Gas chromatography Keywords: Drinking water Keywords: Sodium Sulfate Chinese. Date revised - 2012-03-01. Last updated - 2013-05-06. DOI - f37e5fca-b018-4af8-a481csaobj201; 16176367; 1001-5914. SubjectsTermNotLitGenreText - Mathematical models; Drinking Water; Chromatographic techniques; Solvents; Sulfates; Sodium; Gas chromatography; Chromatography; Drinking water; Malathion; Detection Limits; Analytical Methods; Gas Chromatography; Sodium Sulfate 141. Chen, L; Chen, Z; Liu, Y; Lopez, T; Sankaran, G; Vega, H; Krieger, R, and Chen, L. Insecticide Residues and Their Selected Biomarkers in Produce: Field Studies Using Malathion and Fenpropathrin in Strawberries. 2011 Mar 6. Rec #: 3560 Keywords: ABSTRACT Notes: Chemical of Concern: MLN Abstract: Keywords: Bioindicators Keywords: Insecticides Keywords: Insecticide residues Keywords: Fragaria Keywords: Biomarkers Keywords: biomarkers Keywords: Malathion English. Date revised - 2013-02-26. Last updated - 2013-02-28. DOI - CPI-6047115; 6047115 142. Chen, L.; Zhao, T. F.; Pan, C. P.; Ross, J. H., and Krieger, R. I. Preformed Biomarkers Including Dialkylphosphates (DAPs) in Produce May Confound Biomonitoring in Pesticide Exposure and Risk Assessment. 2012; 60, 9342-9351. Rec #: 12520 Keywords: HUMAN HEALTH Notes: Chemical of Concern: MLN Abstract: Abstract: Low levels of pesticides and their metabolites/degradates occur in produce when pesticides are used in conventional or organic crop protection. Human dietary and nonoccupational urine biomonitoring studies may be confounded by preformed pesticide biomarkers in the diet. The extent of formation of putative urine biomarkers, including malathion specific (MMA, MDA; malathion mono- and diacids), organophosphorus generic (DMP, DMTP, DMDTP; dimethyl-, dimethylthio-, and dimethydithiophosphate), pyrethroid generic (3-PBA; 3-phenoxybenzoic acid), and captan-specific metabolites (THPI; tetrahydrophthalimide), was measured in produce samples containing the parent pesticide. Every produce sample of 19 types of fruits and vegetables contained biomarkers of potential human exposure. A total of 134 of 157 (85%) samples contained more molar equivalent biomarkers than parent pesticide. Malathion and fenpropathrin were sprayed (1 lb/A), and the time-dependent formation of pesticide biomarkers in strawberries was investigated under field conditions typical of commercial production in California. Malathion and fenpropathrin residues were always below established residue tolerances. Malathion, MMA, and MDA dissipated, while DMP, DMTP, and DMDTP increased, during a 20 day study period following the preharvest interval. The mole ratios of biomarkers/(malathion + malaoxon) were always greater than 1 and increased from day 4 to day 23 postapplication. Fenpropathrin and 3-PBA also dissipated in strawberries during each monitoring period. The mole ratios of 3-PBA/fenpropathrin were always less than 1 and decreased from day 4 to day 14. The absorption of pesticide biomarkers in produce and excretion in urine would falsely indicate consumer pesticide exposure if used to reconstruct dose for risk characterization. Number of Volumes: 36 ISI Document Delivery No.: 002YL 143. Chen, Lina; Yin, Lihua; Song, Fengrui; Liu, Zhiqiang; Zheng, Zhong; Xing, Junpeng, and Liu, Shuying. Determination of pesticide residues in ginseng by dispersive liquidÇôliquid microextraction and ultra high performance liquid chromatographyÇôtandem mass spectrometry. 2013 Feb 15-; 917Çô918, (0): 71-77. Rec #: 1300 Keywords: CHEM METHODS Notes: Chemical of Concern: MLN Abstract: A procedure involving acetonitrile-based extraction combined with dispersive liquidÇôliquid microextraction (DLLME) and detection by ultra high performance liquid chromatographyÇôtandem mass spectrometry (UHPLCÇôMS/MS) was used for determination of 39 pesticides in ginseng. The extraction of pesticide residues in ginseng was performed with acetonitrile, applying QuEChERS methodology, and the extract was further disposed by DLLME method before analyzed by UHPLCÇôMS/MS. The average recoveries ranged from 70 to 120% for 82% of the analytes with RSD lower than 15%. The calibration curves obtained with blank matrices were linear with a correlation coefficient of over 0.99. The limits of detection were between 0.01 and 1.0 ++g/kg. Matrix effects were studied by comparing solvent calibration curves and matrix-matched calibration curves. The results indicate the feasibility of this method for the determination of 39 pesticides in ginseng. Pesticides/ Ginseng/ QuEChERS/ DLLME/ UHPLCÇôMS/MS http://www.sciencedirect.com/science/article/pii/S1570023213000184 144. Chen, P. R.; Tucker, W. P., and Dauterman, W. C. Structure of Biologically Produced Malathion Monoacid. 1969; 17, 86-90. Rec #: 1580 Keywords: CHEM METHODS Notes: Chemical of Concern: MLN 145. Chen, Z-B; Liu, L-H; Ye, Q, and Chen, Z-B. Determination of Organophosphorus and Carbamate Pesticide Residues in Water by Gas Chromatography-Mass Spectrometry. 2009 Feb; 26, (2 ): 156-157. Rec #: 5160 Keywords: CHEM METHODS Notes: Chemical of Concern: MLN Abstract: Abstract: Objective To establish a method for determination of organophosphorus and carbamate pesticide residues in water by GC-MS. Methods Twelve kinds of organophosphorus and carbamate pesticide residues were determined with GC-MS with methylenechloride and petroleum ether (4:1) as extraction solvent. Results The linear ranges of 12 kinds of pesticides were perfect in the range of 0.01-1.0kg/ml, r>0.997, the average rates of recovery were 76.0%-111.0%, and RSD were 5.20%-9.20%, the lowest limit detection (S/N=3) of dichlorvos, chinomethionate, parathion, bromophos, carbophenothion, fenthion, isoprocarb, pirimicarb, aminocarb, thiobencarb, pendimethalin, malathion was 0.009, 0.003, 0.008, 0.001, 0.003, 0.003, 0.0025, 0.009, 0.005, 0.010, 0.010, 0.006 kg/ml respectively. Conclusion This method is simple, rapid, sensitive and can be applied to the simultaneous determination of 12 kinds of organophosphorus and carbamate pesticides in the water. Keywords: Pesticide residues Keywords: Water analysis Keywords: Mass spectroscopy Keywords: Malathion Keywords: petroleum ether Keywords: Pendimethalin Keywords: Agricultural Chemicals Keywords: petroleum residues Keywords: Gas chromatography Keywords: Thiobencarb Keywords: Ethers Keywords: X 24330:Agrochemicals Keywords: pendimethalin Keywords: Dichlorvos Keywords: SW 3050:Ultimate disposal of wastes Keywords: P 2000:FRESHWATER POLLUTION Keywords: Solvents Keywords: dichlorvos Keywords: Carbamate Pesticides Keywords: Aqualine Abstracts; Water Resources Abstracts; Pollution Abstracts; Toxicology Abstracts Keywords: AQ 00003:Monitoring and Analysis of Water and Wastes Keywords: Fenthion Keywords: Pesticides (carbamates) Keywords: Spectrometry Keywords: Pesticides Keywords: Parathion Chinese. Date revised - 2009-05-01. Last updated - 2013-04-19. DOI - MD-0009570294; 9263940; 1001-5914. SubjectsTermNotLitGenreText - Pesticide residues; Solvents; Pesticides (carbamates); Fenthion; Mass spectroscopy; Malathion; petroleum ether; Pendimethalin; Thiobencarb; Gas chromatography; Pesticides; Dichlorvos; Parathion; petroleum residues; dichlorvos; Ethers; Water analysis; pendimethalin; Spectrometry; Agricultural Chemicals; Carbamate Pesticides 146. Cheng, Feng-Ning; Li, Zhao-Yang; Li, Qiao-Ling; Xing, Jian; Zhang, Yan-Chuan, and Cheng, Feng-Ning. Photolysis of Typical Chiral Pesticides. 2011 May; 34, (5): 121-123, 139. Rec #: 6400 Keywords: CHEM METHODS Notes: Chemical of Concern: MLN Abstract: Abstract: 6 chiral pesticides were investigated in the study related to their photolysis characteristics by using mercury lamp as light source. Profenophos, malathion, phenthoate, triadimefon and fenpropathrin each has one chiral center and thus exists as two enantiomers. The photolysis of individual enantiomers from one chiral pesticide showed similar dissipation rates of the two enantiomers. No enantiomerization was observed for 5 chiral pesticides. 3 organophosphorus insecticides were photolyzed very rapidly, whereas fenpropathrin had the slowest photolysis rate with a half-life time of 280 h. Cypermethrin has 3 chiral centers and consists of 8 enantiomers which constitute 4 diastereoisomers that could be separated on a chiral silica-gel column. Further photolysis was performed for a single enantiomer from diastereoisomer IV beta -trans-2. Results indicated that certain amounts of diastereoisomers I, II and III were produced, suggesting isomerization took place along with photolysis process. By summing the concentrations of 4 diastereoisomers the total half-life was calculated to be 66.7 h. Keywords: Photolysis Keywords: cypermethrin Keywords: Organophosphorus compounds Keywords: P 9999:GENERAL POLLUTION Keywords: ENA 09:Land Use & Planning Keywords: light sources Keywords: Pesticides Keywords: Mercury Keywords: Pollution Abstracts; Environment Abstracts Keywords: Malathion Chinese. Date revised - 2011-09-01. Last updated - 2012-04-26. DOI - 296015da-1b8c-445a-a6aecsaobj201; 15434384; 1003-6504. SubjectsTermNotLitGenreText - Photolysis; cypermethrin; Organophosphorus compounds; light sources; Pesticides; Mercury; Malathion 147. Cheng, Hao; Li, Jianping; Gao, Xiaoguang; Jia, Jian; Zhang, Dexin; Zhao, Dongjie, and Cheng, Hao. Malathion Detection Method Using Microhotplate-Based Preconcentrator and Ion Mobility Spectrometer. 2012 Jan; 92, (3): 279-288. Rec #: 2940 Keywords: CHEM METHODS Notes: Chemical of Concern: MLN Abstract: Abstract: A simple and rapid method using a microhotplate-based preconcentrator and an ion mobility spectrometer (IMS) is proposed for the detection of malathion in water. The preconcentrator is prepared by micro-electro-mechanical system (MEMS) process. Coated with Polydimethylsiloxane (PDMS), it has the advantages of solvent-less, low energy cost, self-heating and ease to combine with IMS. The operating conditions of the preconcentrator-IMS system, such as extraction time, extraction temperature, agitation speed and desorption temperature, were optimised. Using the preconcentrator, the sampling procedure can be simplified and the detection limit of the system can be decreased. A linear relationship between the IMS response and the concentration of the analyte solution was verified. The malathion detection limit based on 3 times the baseline noise is 0.43 mu g L-1 and the total analysis time is less than 30 minutes. Keywords: Desorption Keywords: Mobility Keywords: Temperature Keywords: Noise levels Keywords: Environment Abstracts Keywords: ENA 08:International Keywords: Malathion English. Date revised - 2012-04-01. Last updated - 2012-05-18. DOI - f69485c1-6a3a-451b-93acmfgefd107; 16536539; 0306-7319; 1029-0397. SubjectsTermNotLitGenreText - Desorption; Mobility; Noise levels; Temperature; Malathion 148. Chiba, Shoetsu; Ikawa, Toru; Takeshita, Hiroshi; Ichiba, Kazue; Sagi, Morihisa; Mukai, Toshiji, and Anzai, Naohiro. Interactions of Human Organic Anion Transporter 1 (Hoat1) With Substances Associated With Forensic Toxicology. 2011 Jul; 13, (4): 180-185. Rec #: 6280 Keywords: HUMAN HEALTH Notes: Chemical of Concern: MLN Abstract: Abstract: Renal excretion is an important elimination pathway for substances associated with forensic toxicology, such as medicines, agricultural chemicals, and industrial chemicals. This study aimed to elucidate the renal elimination pathway of substances using culture cells stably expressing the human organic anion transporter 1 (hOAT1) gene. Substances tested were diazepam, triazolam, haloperidol, amitriptyline, mianserin, bromovalerylurea, phenobarbital, acetaminophen, acetylsalicylic acid, lidocaine, aconitine, atropine, caffeine, nicotine, malathion, dichlorvos, fenitrothion, chlorpyrifosmethyl, paraquat, diquat, potassium cyanide, sodium arsenite, sodium azide, o-cresol, and probenecid (control, a representative inhibitor of hOAT1). Results demonstrated that diazepam, triazolam, amitriptyline, mianserin, malathion, fenitrothion, chlorpyrifosmethyl, and probenecid significantly inhibited representative substrates of hOAT1 and para-aminohippuric acid uptake by hOAT1. IC(50) values of the aforementioned substances were 133.3, 185.2, 354.1, 312.6, 114.2, 26.6, 191.5, and 7.9μM, respectively. Ki values were 83.5, 86.0, 573.9, 99.0, 134.0, 51.2, 324.6, and 9.1μM, respectively. In conclusion, the current results suggest that fenitrothion and chlorpyrifosmethyl are transported with pharmacokinetics indicative of hOAT1 involvement in the human kidney. 2011 Elsevier Ireland Ltd. All rights reserved. Keywords: Animals Keywords: Kidney -- metabolism Keywords: Humans Keywords: Index Medicus Keywords: Mice Keywords: Central Nervous System Agents -- pharmacokinetics Keywords: Organic Anion Transporters Keywords: SLC22A13 protein, human Keywords: 0 Keywords: Insecticides Keywords: Transfection Keywords: Cells, Cultured Keywords: Insecticides -- pharmacokinetics Keywords: Forensic Toxicology Keywords: Organic Anion Transporters -- metabolism Keywords: Central Nervous System Agents eng. Date completed - 2011-10-24. Date created - 2011-05-30. Date revised - 2012-12-20. Last updated - 2013-01-19. DOI - MEDL-21561794; 21561794; 1873-4162 149. Choi, J Y; Yang, D B; Lee, S-G; Bang, J H; Hong, G H; Shin, Kh, and Choi, J Y. Organophosphorous Pesticide Distribution in Seawater From Asan Bay, Korea in 2008. 2010 Sep; 32, (3): 203-212. Rec #: 6860 Keywords: FATE Notes: Chemical of Concern: MLN Abstract: Abstract: Distribution of organophosphorous pesticides (OPs) was studied from February to September 2008 in the seawater of Asan Bay, Korea. Among the 29 types of OPs detected during the study period, IBP(S-benzyl O,O-diisopropyl phosphorothioate), ranging from <1 ng/l to 377 ng/l, was the most abundant. Other commonly observed OPs concentrations in the study area included diazinon (Diethyl 2-isopropyl-4-methyl-6-pyrimidinyl phosphorothionate; <16307 ng/l), azinphos ethyl (3,4-Dihydro-4-oxo-3-benzotriazinyl-methyl O,O-diethyl phosphorodithioate; <161997 ng/l), malathion (1,2-Di(ethoxycarbonyl)ethyl O,O-dimethyl phosphordithioates; <163013 ng/l), demeton-O (Diethyl 2-(ethylthio)ethyl phosphorothionate; <162403 ng/l), and DDVP (2,2-dichlorovinyl dimethyl phosphate; <16283 ng/l). Seasonal distribution of OPs in seawater is dependent on the OP application period. In August, OPs concentrations were generally decreased with the increased salinity of seawater, implying progressive dilution of pesticides in the estuarine system. OPs were deposited into Asan Bay from Asan and Sabkyo Lakes as well as surrounding tributaries. Ten OPs, including diazinon, were detected in the suspended particles of Asan Bay. Keywords: Oceanic Abstracts; ASFA 3: Aquatic Pollution & Environmental Quality Keywords: Q5 01503:Characteristics, behavior and fate Keywords: Marine Keywords: Estuaries Keywords: Brackish Keywords: Seasonal distribution Keywords: Suspended particulate matter Keywords: INW, Korea, Rep., Asan Bay Keywords: Sea water Keywords: Korea, Rep., Asan Keywords: Pesticides Keywords: INW, Korea, Rep. Keywords: Brackishwater environment Keywords: Tributaries Keywords: O 4060:Pollution - Environment Korean. Date revised - 2010-11-01. Last updated - 2013-04-19. DOI - MD-0014985668; 13970439; CS1314501; 1598-141X. SubjectsTermNotLitGenreText - Sea water; Estuaries; Pesticides; Brackishwater environment; Seasonal distribution; Suspended particulate matter; Tributaries; Korea, Rep., Asan; INW, Korea, Rep.; INW, Korea, Rep., Asan Bay; Marine; Brackish 150. Chowdhury, M. A. Z.; Banik, S.; Uddin, B.; Moniruzzaman, M.; Karim, N., and Gan, S. H. Organophosphorus and Carbamate Pesticide Residues Detected in Water Samples Collected from Paddy and Vegetable Fields of the Savar and Dhamrai Upazilas in Bangladesh. 2012; 9, 3318-3329. Rec #: 12620 Keywords: CHEM METHODS Notes: Chemical of Concern: MLN Abstract: Abstract: Several types of organophosphorous and carbamate pesticides have been used extensively by the farmers in Bangladesh during the last few decades. Twenty seven water samples collected from both paddy and vegetable fields in the Savar and Dhamrai Upazilas in Bangladesh were analyzed to determine the occurrence and distribution of organophosphorus (chlorpyrifos, malathion and diazinon) and carbamate (carbaryl and carbofuran) pesticide residues. A high performance liquid chromatograph instrument equipped with a photodiode array detector was used to determine the concentrations of these pesticide residues. Diazinon and carbofuran were detected in water samples collected from Savar Upazila at 0.9 mu g/L and 198.7 mu g/L, respectively. Malathion was also detected in a single water sample at 105.2 mu g/L from Dhamrai Upazila. Carbaryl was the most common pesticide detected in Dhamrai Upazila at 14.1 and 18.1 mu g/L, while another water sample from Dhamrai Upazila was contaminated with carbofuran at 105.2 mu g/L. Chlorpyrifos was not detected in any sample. Overall, the pesticide residues detected were well above the maximum acceptable levels of total and individual pesticide contamination, at 0.5 and 0.1 mu g/L, respectively, in water samples recommended by the European Economic Community (Directive 98/83/EC). The presence of these pesticide residues may be attributed by their intense use by the farmers living in these areas. Proper handling of these pesticides should be ensured to avoid direct or indirect exposure to these pesticides. Number of Volumes: 9 ISI Document Delivery No.: 012XP 151. Ciliberti, A.; Berny, P.; Vey, D., and de Buffrenil, V. Assessing environmental contamination around obsolete pesticide stockpiles in West Africa: Using the Nile monitor (Varanus niloticus) as a sentinel species. 2012; 31, 387-394. Rec #: 12640 Keywords: SURVEY Notes: Chemical of Concern: MLN Abstract: Abstract: Environmental contamination caused by obsolete pesticide stocks was assessed using the Nile monitor (Varanus niloticus) as a sentinel species. Organochlorines and organophosphates were quantified by gas chromatography in abdominal fat and the liver, respectively. Results were compared to those obtained from three other sites, characterized by different histories of contamination. None of the previously stocked pesticides were recovered. Low to moderate levels of 4,4'-dichlorodiphenyldichloroethylene (4,4'-DDE) were quantified in monitors from all sites. Malathion and 4,4'-dichlorodiphenyldichloroethane (4,4'-DDD) also were detected sporadically. Interindividual variability was substantial. Correlations between pesticide loads and individual characteristics were considered. The nondetection of previously stocked pesticides in the monitors' tissues, their contamination by other pesticides, and the value of V. niloticus as a monitoring tool for environmental contamination are discussed. The results indicate a situation of low concern and draw attention to the importance of local conditions in determining environmental dangers associated with potential pollution sources. Environ. Toxicol. Chem. 2012;31:387-394. (C) 2011 SETAC Number of Volumes: 2 ISI Document Delivery No.: 875NB 152. Clayson, P. J.; Latham, M.; Bonds, J. A. S.; Healy, S. P.; Crans, S. C., and Farajollahi, A. A DROPLET COLLECTION DEVICE AND SUPPORT SYSTEM FOR ULTRA-LOW-VOLUME ADULTICIDE TRIALS. 2010; 26, 229-232. Rec #: 12650 Keywords: METHODS Notes: Chemical of Concern: MLN Abstract: Abstract: A supporting stand to suspend rotating impactors and mosquito cages is a requirement for field tests during pesticide efficacy trials. We present schematics for a collection device and associated support system for sampling droplets of ultra-low-volume (ULV) sprays during mosquito Directory: pesticides -> nas nas -> Appendix 1-10: Summary of Diazinon Monitoring Data nas -> Description of revision nas -> Appendix 2 Chlorpyrifos Species Sensitivity Distribution Analysis for Fish nas -> Appendix 2 Open Literature Review Summaries for Malathion Download 5.18 Mb. Share with your friends:
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