Abstract: Occurrence and biomass of algae growing epiphytically on deepwater rice plants were studied over a flood period in 1985 near Sonargaon, Bangladesh. Biomass estimation and determination of cell volume are described. The epiphytic flora was dominated by blue-green algae, green algae and diatoms. Maximum biomass was observed about 70 days after flooding. Heterocystous blue-green algae predominated and the most abundant genus was Gloeotrichia followed by Microchaete, Hapalosiphon, Nostoc , etc. Aphanothece pallida is the only non-heterocystous blue-green alga. Green algae were the next important group and the dominant genus was Oedogonium followed by Spirogyra, Rhizoclonium, Coleochaete , etc. Gomphonema is the only diatom genus found as an epiphyte on deepwater rice plants.
Abstract: Fifty cyanobacterial strains (10 genera) were tested in batch culture for their ability to use org. phosphorus compds. (1 mg liter-1 P) as their sole P source. Two monoesters, Na2-b-glycerophosphate and p-nitrophenyl phosphate (pNPP), supported growth of all strains, and the diester bis-p-nitrophenyl phosphate (bis-pNPP) and herring sperm DNA supported almost all strains. ATP was either a very favorable or poor P source and failed to support growth of 9 strains, 7 of which were Rivulariaceae with trichomes ending in a hair or long tapered region. Phytic acid was in general the least favorable P source. P-limited cultures grown initially with inorg. phosphate to conditions of P limitations were also tested for cell-bound and extracellular phosphomonoesterase (PMEase) and phosphodiesterase (PDEase) activities at 2 pH values (7.6, 10.3) using pNPP and bis-pNPP as substrates. Cell-bound PMEase was inducible in all strains and cell-bound PDEase in most strains. Most showed extracellular PMEase, but not extracellular PDEase. The highest values (mM pNPP or bis-pNPP hydrolyzed mg dry wt.-1 h-1) all occurred in strains of Gloeotrichia as follows: cell-bound PMEase at pH 7.6, 2.7 mM in strain D602; cell-bound PMEase at pH 10.3, 5.2 mM in D602; extracellular PMEase at pH 7.6, 0.73 mM in D281; extracellular PMEase at pH 10.3, 6.6 mM in D281; cell-bound PDEase at 7.6, 0.40 mM in D613; cell-bound PDEase at pH 10.3, 1.0 mM in D613. The results were compared to see if they indicated possible relationships between phosphatase activity and taxonomic or ecol. grouping. Rivulariaceae produced higher yields than filamentous non-Rivulariaceae with b-glycerophosphate, pNPP, and DNA. Rivulariaceae with the ability to form hairs in culture showed poorer growth in ATP than non-hair-forming Rivulariaceae, but were more effective at utilizing phytic acid. Strains from calcareous environments had higher PMEase activity at pH 10.3 than strains from noncalcareous environments (P < 0.01). [on SciFinder (R)]
Abstract: We used laboratory feeding trials to examine the responses to copepods to a variety of cyanobacteria of differing size, morphology, and toxicity. Diaptomus birgei exhibited behaviors ranging from strong preferences for some taxa of cyanobacteria to almost complete rejection of other taxa when allowed to feed in low-concentration mixtures of a high-quality green alga, Chlamydomonas reinhardi, and each of 16 taxa of cyanobacteria. Our results demonstrate the overriding importance of chemical factors. Copepods exhibited very high clearance rates for some filaments and colonies but rejected morphologically similar strains shown to contain potentially toxic compounds. Responses to resource abundance exhibited three patterns, each consistent with the assumptions and predictions of an optimal diet model. First, selectivity for high-ranking cyanobacteria declined in high-concentration mixtures with Chlamydomonas relative to selectivity in low-concentration mixtures. Second, a test of the effects of time without food showed that hunger, rather than food concentration per se, was responsible for these shifts in selectivity. Finally, each toxic strain was consistently ingested at very low rates, even when offered as the sole food resource. Experiments with complex mixtures of natural seston revealed similar patterns of consistently strong discrimination against a presumably toxic cyanobacterium and concentration-dependent selectivity for presumably nontoxic taxa. Our results show how the behavioral flexibility of copepods can be adaptive in lakes with significant populations of cyanobacteria. The ability of copepods to feed size-selectively on filaments and colonies and to discriminate against toxic forms suggests that these grazers may be important in the evolution and continued success of toxic strains.
K 03059 Algae; W 30511 Plant breeding and aquaculture; Q4 27310
Plant culture; Q1 01625 Non-edible products
Abstract: The large scale production of nitrogen fixing cyanobacteria is discussed and the use of ammonia excreting mutants of Anabaena siamensis is described. Gloeotrichia natans is considered for use as a biofertilizer and for the production of phycobiliproteins.
Notes: 1991
0032-079x
English
Journal Article
Author Address: Micro-Algal Biotechnol. Lab., Jacob Blaustein Inst. Des. Res., Ben-Gurion Univ., Sede Boker 84990, Israel
Reference Type: Journal Article
Record Number: 188
Author: Al-Hakeem, T. M.; Al-Salihi, F. G.; Al-Samaraie, A. T.
Year: 1991
Title: Some essential constituents of N sub(2)-fixing cyanobacteria isolated from Shatt Al-Arab at Garma Region, south Iraq
heavy metals; Cyanophyta; Gloeotrichia natans; Gloeotrichia;
Nostoc microscopicum; Nostoc commune
ISW, Iraq, Shatt Al Arab
Marine
Q1 01226 Physiology, biochemistry, biophysics
Abstract: To the 4 N sub(2) - fixing species: Gloeotrichia natans, Gloeotrichia spp., Nostoc microscopiccus and N. commune, which had been isolated, purified and identified from Shatt Al-Arab at Garma Region (Iraq) heavy metals, total lipids content and free amino acids were quantitatively determined. The concentration of Mn and Cu are in agreement with the required values found in other cyanobacteria, while Ni and Zn seems to be above that value. Iron concentration less than the required value, while Co is undetected. Results show high lipid content of these species. Free amino acids analysis in the isolated species shows the presence of most of the essential and non-essential amino acids. Due to the high protein and lipid contents, these cyanobacteria might be used as food substitutes.
Gloeotrichia nitrogen fixation rice field phycobiliprotein
Abstract: G. natans, a nitrogen-fixing cyanobacterium common in rice fields in the Philippines, was used for studies to establish key features of its physiol. and potential prodn. in outdoor cultures. Under optimal growth conditions (38 Deg, pH 8.0, no carbon enrichment) the specific growth rate of rice-field isolate was 0.076/h. The pH of the medium (between 6.5 and 9.0) did not influence the growth rate, but it did affect phycobiliprotein content, as reflected by a change in color. At pH 7.0 the culture was green-brown, with phycobiliproteins constituting up to 10% of the total protein, while at pH 9.0 the culture was brownish-black and the pigment content was as high as 28% of the total protein. In outdoor cultures the specific growth rate was related directly to cell d. at half this rate. At a stirring of 30 rpm, daily prodn. of outdoor cultures harvested to maintain cell densities of 0.7, 1.15 and 1.5 g L-1 were 14.7, 17.1 and 18.1 g m-2 day-1, resp. This rate of prodn. was maintained for more than 45 days. Phycobiliprotein content in the culture kept at a d. of 1.5 g L-1 reached 14% of the total biomass. [on SciFinder (R)]
Abstract: Mutants of Gloeotrichia ghosei fil1 het++ and pol- het- failed to grow on molecular nitrogen and required combined nitrogen sources. NO3- and NH4+ supported maximum growth of parent and mutant strains under aerobic conditions but not under reducing anaerobic or heterotrophic conditions. Glutamine and tryptophan were superior for the parent strain compared to inorganic nitrogen sources. By mutants only glutamine was used whereas tryptophan was inhibitory to them. Development of heterocyst and polarity in the parent and in fil1 het++ was inhibited in the presence of all combined nitrogen sources compared to molecular nitrogen which supported their formation. MSX and AZT (analogues of glutamine and tryptophan, respectively) completely inhibited the growth of all strains in the nitrogen-fixing and NH4(+)-medium. When glutamine and tryptophan were added to MSX- and AZT-containing medium, growth of parent strain was similar to that of the respective amino acids. The mutants grew only with glutamine + MSX, and tryptophan + AZT complex medium inhibited growth. [on SciFinder (R)]
Notes: Centre of Advanced Study in Botany, Banaras Hindu University, Varnasi, India
0231-4622
Hungary
Journal; Article; (JOURNAL ARTICLE)
English
Reference Type: Journal Article
Record Number: 190
Author: Mishra, A. K.; Tiwari, D. N.
Year: 1990
Title: Reversion of Mutation in Non-Nitrogen-Fixing Mutants of the Cyanobacterium Gloeotrichia-Ghosei
Author Address: BANARAS HINDU UNIV,CTR ADV STUDY BOT,VARANASI 221005,UTTAR PRADESH,INDIA.
Reference Type: Journal Article
Record Number: 192
Author: Lebedev, N. N.; Chan Van, Ni; Khatypov, R. A.; Krasnovskii, A. A.
Year: 1990
Title: Energetic interaction of phycobilins and chlorophyll-protein complexes in cyanobacteria cells: effect of thermoinactivation
Journal: Biofizika
Volume: 35
Issue: 1
Pages: 62-8
Accession Number: AN 1990:195419
Keywords: Gloeotrichia raciborski; Nostoc commune (chlorophyll-protein complexes and phycobilins in, energetic interaction of, heat inactivation effect on); Biliproteins Role: BIOL (Biological study) (energetic interactions of chlorophyll-protein complexes and, in cyanobacteria, heat inactivation effect on); Heat (on photosynthetic energetic interactions of phycobilins and chlorophyll-protein complexes in cyanobacteria); Photosynthetic systems (II, of cyanobacteria, heat-induced rearrangements in); Chlorophylls Role: BIOL (Biological study) (complexes, with proteins, phycobilins energetic interaction with, in cyanobacteria, heat inactivation effect on)
phycobilin chlorophyll protein complex cyanobacteria heat
Abstract: Spectra and lifetimes of low-temp. fluorecence in Gloeotrichia raciborski and Nostoc commune were investigated. Heating of cyanobacteria to 50-55 Deg for 10 min results in an increase of the intensity of bands at 663, 686, and 697 nm when illuminated with light absorbed by the phycobilins; there is a decrease of the bands at 686 and 697 nm and initiation of a band at 673 nm when illuminated with light absorbed by chlorophyll. The increase of the band at 686 nm is accompanied by an increase in the lifetime of its slow component from 880 to 1250 ps. The lifetime of the slow component of the band at 673 nm is 900 ps. Heating may disturb the interaction between pigment-protein complexes, which could result in structural rearrangements in photosystem II and splitting of phycobilisomes. [on SciFinder (R)]
Notes: CAN 112:195419
11-6
Plant Biochemistry
A. N. Bakh Inst. Biochem.,Moscow,USSR.
Journal
0006-3029
written in Russian.
Reference Type: Journal Article
Record Number: 193
Author: Chu, Fun S.; Huang, Xuan; Wei, R. D.
Year: 1990
Title: Enzyme-linked immunosorbent assay for microcystins in blue-green algal blooms
Journal: Journal - Association of Official Analytical Chemists
Volume: 73
Issue: 3
Pages: 451-6
Accession Number: AN 1990:453823
Keywords: Algae; Gloeotrichia; Lyngbya; Microcystis; Microcystis aeruginosa (microcystin detn. in, by ELISA)
microcystin detn algae water ELISA
Abstract: A direct competitive ELISA for the freshwater blue-green algal toxin microcystin (MCYST) in algae and water was developed. The assay involves coating anti-MCYST-variant leucine-arginine (LR) antibody to the ELISA plate and the use of MCYST-LR peroxidase as the enzyme marker. The linear portion of the std. curve for MCYST in phosphate buffer contg. saline (PBS) was 0.5-10.0 ng/mL (25-500 pg/assay). The min. detection level for MCYST-LR was 0.20 ng/mL (10 pg/assay). Contaminated water could be directly used in the ELISA. The overall anal. recoveries for MCYST-LR added to water at levels of 1-20 ng/mL was 83.4%. For anal. of cellular MCYST, the toxin was first extd. from the algae with 0.1M ammonium bicarbonate, dild. with PBS to less than 0.5 mg dried algae/mL (<5.0 mg wet wt./mL), and directly used in the ELISA. C-18 reversed-phase Sep-Pak cartridges effectively adsorbed MCYST from the toxin-contg. solns. The toxin could be recovered from the cartridge by eluting with 60% methanol. Using this approach, algae ext. that was relatively free of MCYST was prepd. and was used in a recovery study. The overall anal. recovery of MCYST added to the algae ext. in the range 0.25-20 ppm was 83% with a coeff. of variation of 11.9%. The detection limit for MCYST in dried algae was about 0.25-0.5 mg/g (0.25-0.5 ppm) lyophilized algae sample. This method was applied for the anal. of several naturally occurring algae blooms. Limited samples were also analyzed for MYCST by liq. chromatog. ELISA data were in general agreement with those obtained by liq. chromatog. MCYST concns. from 0.006 to 2.9 mg/g (6 to 2900 ppb) and from 26 to 5200 mg/g (26 ppm to 5200 ppm) were found in water and algae (dried wt.), resp. [on SciFinder (R)]
Notes: CAN 113:53823
4-1
Toxicology
Food Res. Inst.,Univ. Wisconsin,Madison,WI,USA.
Journal
0004-5756
written in English.
77238-39-2 (Microcystin); 101043-37-2 (Microcystin LR) Role: ANT (Analyte), ANST (Analytical study) (detn. of, in algae and water by ELISA); 7732-18-5 (Water) Role: AMX (Analytical matrix), ANST (Analytical study) (microcystin detn. in, by ELISA)
Reference Type: Journal Article
Record Number: 103
Author: Bartley, D. D.; Morgan, A. V.
Year: 1990
Title: The Palynological Record of the King's Pool, Stafford, England
Journal: New Phytologist
Volume: 116
Issue: 1
Pages: 177-194
Date: Sep.
Keywords: Stafford, Pollen Analysis, Vegetation History
Abstract: The King's Pool is an elongated depression in which up to 21 m of organic sediments have been found. Details of the sediments and the macrofossils are given. The pollen diagram is presented in both percentage and concentration forms, and these show considerable differences especially for the Late Devensian period. The sequence begins at about 13 000 years BP though the pollen diagram begins near the end of the Windermere interstadial. It appears that there are a number of hiatuses in the sequence, the most important spanning most of the Atlantic period (Godwin pollen zone VIIa). Forest clearance is evident from early in the Neolithic period with further clearance in the Bronze Age (shown most clearly in the concentration diagram) but with the greatest clearance at the beginning of the Iron Age. This latter clearance is accompanied by increased productivity of the lake, indicated by organic remains, including abundant blue-green algae, suggesting eutrophication. Arable agriculture including cultivation of cereals and Cannabis seems to have increased near the end of the Roman period.